ACYP1 Antibody

Western Blot: ACYP1 Antibody [NB100-57091] - (0.01ug/ml) staining of Human Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ACYP1 Antibody Summary

• Description: Novus Biologicals Goat ACYP1 Antibody (NB100-57091) is a polyclonal antibody validated for use in WB and ELISA. Anti-ACYP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PKSHIDKANFNNEK corresponding to internal region according to NP_001098.1.
• Specificity: This antibody is expected to recognize isoform a (NP_001098.1) only.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ACYP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 15 kDa band observed in human testis lysates (calculated MW of 11.3 kDa band according to NP_001098.1).
• Control:

  ACYP1 Overexpression Lysate
  Human Testis Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ACYP1 Antibody

• acylphosphatase 1, erythrocyte (common) type
• Acylphosphatase, erythrocyte isozyme
• Acylphosphatase, organ-common type isozyme
• Acylphosphate phosphohydrolase 1
• ACYPEacylphosphatase-1
• EC 3.6.1.7

Background

Acylphosphatase is a small cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase, on the basis of their tissue localization. This gene encodes the erythrocyte acylphosphatase isoenzyme. Alternatively spliced transcript variants that encode different proteins were identified through data analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ACYP1 Antibody (NB100-57091)(1)

Publication using NB100-57091

• Paoli P, Pazzagli L, Giannoni E et al. A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase. J Biol Chem 2003-01-03 [PMID: 12409302]

Bioinformatics

• Gene Symbol: ACYP1
• Entrez:
  • 97(Human)
  • 66204(Mouse)
• Uniprot:
  • P07311(Human)