| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NBP1-30054AFP405 | |||
| Alexa Fluor Plus 488 | NBP1-30054AFP488 | |||
| Alexa Fluor Plus 555 | NBP1-30054AFP555 | |||
| Alexa Fluor Plus 594 | NBP1-30054AFP594 | |||
| Alexa Fluor Plus 647 | NBP1-30054AFP647 | |||
| Alexa Fluor Plus 680 | NBP1-30054AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-30054-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-30042SS | |||
![Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the collagen protein with an observed molecular weight ~140.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Western-Blot-NBP1-30054-img0005.jpg "Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Rat lung lysate showing specific immunolabeling of the collagen protein with an observed molecular weight ~140.")
![Immunohistochemistry: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Collagen I Antibody [NBP1-30054] - Formaldehyde-fixed fibrotic mouse lung tissue. The antibody recognizes mature collagen I (red) that has formed fibrils in the extracellular matrix.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30054-img0004.jpg)
![Simple Western: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - Simple Western lane view shows a specific band for Collagen I Alpha 1 in 0.5 mg/ml of Human Lung (left) and Human Kidney (right) lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody---Azide-and-BSA-Free-Simple-Western-NBP1-30054-img0003.jpg)
![Western Blot: Collagen I alpha 1 Antibody - Azide and BSA Free [NBP1-30054] - TNC deficiency reduced kidney fibrosis in animal models.Deletion of TNC (TNC−/−) significantly attenuated the induction of collagen I assessed by IF following UUO by approximate 30% at day 7 & 10 compared with their wild-type littermates (A, n = 4 for each time point, two-way ANOVA p < 0.05). Consistently, the expression of fibrosis markers, such as collagen I alpha , fibronectin & plasminogen activator inhibitor-1 (PAI-1), were significantly lower in TNC−/− mice at UUO day 7 & 10 (B, n = 4 for each time point, two-way ANOVA, p < 0.05). Western blot showed that the proteins of collagen I alpha & alpha -SMA were also significantly reduced in TNC−/− mice at UUO day 7 (C, n = 7, p < 0.05). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36522320), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30054_rabbit-polyclonal-collagen-i-alpha-1-antibody-31020241534311.jpg)
![Immunohistochemistry: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Analysis of periostin in rabbit periosteum. Image courtesy of product review submitted by Samer Zaky.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Immunohistochemistry-NBP1-30042-img0003.jpg)
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Rat lung lysate showing specific immunolabeling of the ~ 93 kDa periostin protein doublet.](http://images.novusbio.com/fullsize/Periostin-OSF-2-Antibody---C-terminus---Azide-and-BSA-Free-Western-Blot-NBP1-30042-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - Cardiac muscle phenotypes of mice at 12 weeks of age.(A) Evaluation of cardiac function by transthoracic echocardiographic analyses. Left ventricular ejection fractions (LVEFs) are shown (n = 8–9). (B) H&E staining of cryosections from cardiac muscle. (C) Immunostaining of laminin ɑ2 (green) & periostin (red) with DAPI (blue) from cardiac muscle. (D) Western blot analyses of periostin. The graph shows the quantification of periostin levels normalized to GAPDH (n = 3). (E) qPCR analyses of Tgfb2 (TGF beta 2), Postn (periostin), & Fn1 (fibronectin) mRNA levels in cardiac muscle (n = 4). (F) qPCR analyses of Nppa (natriuretic peptide A) & Nppb (natriuretic peptide B) mRNA levels in cardiac muscle (n = 4). In qPCR analyses, data were normalized by Gapdh mRNA & expressed as fold increases from WT mice. ***P<0.001 compared with WT mice. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31430335), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30042_rabbit-polyclonal-periostin-osf-2-antibody-c-terminus-31020241616035.jpg)
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - TGFBI & periostin are induced in the heart after injury.(A) Western blot analysis of periostin & TGFBI in isolated adult cardiomyocytes & fibroblasts. Sarcomeric alpha -actin was used as a control for cardiomyocyte purity & GAPDH was used as a loading control. (B) Quantitative real time PCR for Postn & Tgfbi from 1 week sham or MI-operated hearts. mRNA levels were normalized to 18s ribosomal RNA. *p<0.05 for both genes in MI-operated animals compared to sham animals using an unpaired Student’s T-test. n = 3 animals. (C) Western blot analysis for periostin & TGFBI in the infarcted areas isolated from hearts 24 hours, 7 & 14 days after MI surgery. GAPDH was used as a loading control. Each lane corresponds to protein from one mouse. (D & E) Quantification of TGFBI (D) & periostin (E) protein levels from the conditions shown in panel C except that 3 hearts were analyzed in total each. (F) Immunohistochemistry for the indicated markers/proteins on sham or MI-operated hearts after 1 week. Images were taken at 400x magnification. Scale = 20 μm. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0181945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30042_rabbit-polyclonal-periostin-osf-2-antibody-c-terminus-31020241616068.jpg)
![Western Blot: Periostin/OSF-2 Antibody - C-terminus - Azide and BSA Free [NBP1-30042] - PostnMCM allele activity in vivo.(a) Schematic representation of the Postn genetic locus with a tamoxifen-regulated MerCreMer cDNA cassette inserted into exon 1 (E1), which was crossed with Rosa26 reporter mice (R26-eGFP) containing loxP sites flanking a stop cassette upstream of eGFP to allow for Cre-dependent lineage tracing. (b) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were given tamoxifen for 8 weeks before harvesting at 16 weeks. (c) Representative histological section from the heart of mice described in a & b, which show exceptionally rare labelling of interstitial cells at baseline (arrow) with 8 weeks of tamoxifen. Nuclei are stained in blue. Inset shows alpha -actin stained cardiomyocytes (red) surrounding the one eGFP-labelled interstitial cell (green) (n=4 mice). (d) Experimental scheme whereby PostnMCM/+; R26-eGFP mice were MI injured or subjected to a sham procedure, then given tamoxifen for 1 week before harvesting. (e) Western blot analysis for periostin, eGFP, Cre (MerCreMer protein) & GAPDH as a control at day 0 before injury or day 7 after MI injury with 1 week of tamoxifen (n=3 mice per condition). (f) Representative histological sections showing eGFP-labelled interstitial cells in hearts of PostnMCM/+; R26-eGFP after MI injury with 7 days of tamoxifen labelling, but not with a sham procedure (n=6 mice for MI & n=3 for sham). (g) Whole-mount fluorescent images of hearts from PostnMCM/+; R26-eGFP mice for direct eGFP fluorescence over the given time course shown. A no tamoxifen control 7 days after MI is also shown (n=3 mice per time point & condition). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27447449), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-30042_rabbit-polyclonal-periostin-osf-2-antibody-c-terminus-310202416163715.jpg)
![Western Blot: P4HA1 Antibody [H00005033-B01P] - Analysis of P4HA1 expression in transfected 293T cell line by P4HA1 polyclonal antibody. Lane 1: P4HA1 transfected lysate(58.74 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/P4HA1-Antibody-Western-Blot-H00005033-B01P-img0003.jpg "Western Blot: P4HA1 Antibody [H00005033-B01P] - Analysis of P4HA1 expression in transfected 293T cell line by P4HA1 polyclonal antibody. Lane 1: P4HA1 transfected lysate(58.74 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: P4HA1 Antibody [H00005033-B01P] - P4HA1 MaxPab polyclonal antibody. Western Blot analysis of P4HA1 expression in A-431.](http://images.novusbio.com/fullsize/P4HA1-Antibody-Western-Blot-H00005033-B01P-img0002.jpg)
![Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat hippocampal lysate showing specific labeling of the ~120 kDa TAO Kinase 2 (phospho-Ser181) band.](http://images.novusbio.com/fullsize/TAO2-[p-Ser181]-Antibody-Western-Blot-NB300-233-img0005.jpg "Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat hippocampal lysate showing specific labeling of the ~120 kDa TAO Kinase 2 (phospho-Ser181) band.")
![<b>Biological Strategies Validation. </b>Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat cortex lysate showing specific immunolabeling of the ~120k TAO2 phosphorylated at Ser181 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubatede (1200 units for 30 min) before being exposed to the Ser181 TAO2 antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/TAO2-[p-Ser181]-Antibody-Western-Blot-NB300-233-img0004.jpg)
![Western Blot: Collagen I alpha 1 Antibody [NBP2-29651] - Rat lung lysate showing specific immunolabeling of collagen I alpha 1 with observed molecular weight at ~180.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Western-Blot-NBP2-29651-img0005.jpg "Western Blot: Collagen I alpha 1 Antibody [NBP2-29651] - Rat lung lysate showing specific immunolabeling of collagen I alpha 1 with observed molecular weight at ~180.")
![Immunohistochemistry: Collagen I alpha 1 Antibody [NBP2-29651] - Collagen I Antibody [NBP2-29651] - fibrotic mouse lung tissue showing specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized.](http://images.novusbio.com/fullsize/Collagen-I-alpha-1-Antibody-Immunohistochemistry-NBP2-29651-img0004.jpg)
![Western Blot: GAP43 [p Ser41] Antibody [NB300-179] - Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/GAP43-[p-Ser41]-Antibody-Western-Blot-NB300-179-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: GAP43 [p Ser41] Antibody [NB300-179] - Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with lambda-phosphatase.")
![Western Blot: PTPN3 [p Ser459] Antibody [NBP2-29518] - Western blot of mouse testes lysate showing specific immunolabeling of the ~104k PTPH1 phosphorylated at Ser459 (Control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min, RT) before being exposed to the phospho-Ser459 PTPH1 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/PTPN3-[p-Ser459]-Antibody-Western-Blot-NBP2-29518-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: PTPN3 [p Ser459] Antibody [NBP2-29518] - Western blot of mouse testes lysate showing specific immunolabeling of the ~104k PTPH1 phosphorylated at Ser459 (Control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (400 units/100ul lysate for 30 min, RT) before being exposed to the phospho-Ser459 PTPH1 antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: CtIP [p Thr847] Antibody [NBP2-60770] - Western blot of human T47D cell lysate showing specificimmunolabeling of the ~100 kDa CtIP phosphorylated at Thr847 in the first lane (-). Phosphospecificity is shown in the second lane (+)where immunolabeling is completely eliminated by blot treatmentwith lambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/CtIP-[p-Thr847]-Antibody-Western-Blot-NBP2-60770-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: CtIP [p Thr847] Antibody [NBP2-60770] - Western blot of human T47D cell lysate showing specificimmunolabeling of the ~100 kDa CtIP phosphorylated at Thr847 in the first lane (-). Phosphospecificity is shown in the second lane (+)where immunolabeling is completely eliminated by blot treatmentwith lambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")