Note: Mouseover a species abbreviation on the product page to display the fullname.
Find lysates for your research and use the filters to easily sort our lysate products by species, lysate tissue type, lysate subcellular fraction and more. Tissue lysates, whole cell lysates and nuclear lysates are available.
How can we help you?
![Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0005.jpg "Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Mouse Neuoblast cells (Neuro2A) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49688) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.")
![<b>Biological Strategies Validation. </b>Western Blot: Neuro2a Chloroquine Treated / Untreated Cell Lysate [NBP2-49688] - Western Blot: [NBP2-49688] - 10 ug of Chloroquine treated (+) and untreated (-) Neuro2A lysates in 1x Laemmli sample buffer (NBP2-49688) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Neuro2a-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49688-img0004.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0005.jpg "Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with (+) or without 50 uM (-) of Chloriquine (CQ) for 24 hours. Total cell lysates were prepared and separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF membrane and blocked in 5% non-fat milk. The membrane was then probed with 2 ug/ml of p62/SQSTM1 antibody (NBP1-42821) in 1% milk and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.")
![<b>Biological Strategies Validation. </b>Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - 10 ug of Chloroquine treated (+) and untreated (-) HeLa lysates in 1x Laemmli sample buffer (NBP2-49689) were separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml monoclonal anti-LC3 (NBP2--46892) and detected with an anti-rabbit secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0002.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - Human Cervical carcinoma cells (HeLa) were treated with (+) and without (-) 50 uM Chloroquine overnight. Whole cell protein lysates were prepared in 1x Laemmli sample buffer and approximately 10 ug of each lysate (NBP2-49689) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1 ug/ml anti-LC3 (NB100-2220) and 1 ug/ml anti-alpha tubulin (NB100-690) as a loading control, and detected with the appropriate secondary antibodies using chemiluminescence.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0003.jpg)
![Western Blot: HeLa Chloroquine Treated / Untreated Cell Lysate [NBP2-49689] - HeLa cells were treated with vehicle (-) or with 50 uM chloriquine (+) for 24 hours and the total cell lysates were prepared. The lysates were separated on a 12% gel by SDS-PAGE. Protein was transferred to PVDF mebbrane and blocked in 5% non-fat milk. The membrane was then probed with 1 ug/ml of p62/SQSTM1 antibody (NBP1-48320) in 1% milk and dectected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the upregulation of p62 (arrowhead) in response to chloroquine treatment and the blockage of autophagy.](http://images.novusbio.com/fullsize/HeLa-Chloroquine-Treated---Untreated-Cell-Lysate-Western-Blot-NBP2-49689-img0004.jpg)
![Western Blot: HeLa Hypoxic / Normoxic Tissue Lysate [NBP2-36452] - Western blot analysis of HIF-1 alpha antibody (NB100-134) in HeLa hypoxic and normoxic lysates (NBP2-36452). Tubulin loading control is shown below.](http://images.novusbio.com/fullsize/HeLa-Hypoxic---Normoxic-Tissue-Lysate-Western-Blot-NBP2-36452-img0002.jpg "Western Blot: HeLa Hypoxic / Normoxic Tissue Lysate [NBP2-36452] - Western blot analysis of HIF-1 alpha antibody (NB100-134) in HeLa hypoxic and normoxic lysates (NBP2-36452). Tubulin loading control is shown below.")
![Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].](http://images.novusbio.com/fullsize/HepG2-Whole-Cell-Lysate-Western-Blot-NBP1-42569-img0004.jpg "Western Blot: HepG2 Whole Cell Lysate [NBP1-42569] - Analysis of Actin (NB600-532) using HepG2 whole cell lysate [NBP1-42569].")
![SDS-Page: HepG2 Whole Cell Lysate [NBP1-42569] - Ponceau S staining of HepG2 whole cell lysate using NBP1-42569.](http://images.novusbio.com/fullsize/HepG2-Whole-Cell-Lysate-SDS-Page-NBP1-42569-img0001.jpg)
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0003.jpg "Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-449).")
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were left untreated (C) or were treated with Cobalt Chloride / CoCl2 (H) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (clone H1alpha67; catalog# NB100-105).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0001.jpg)
![Western Blot: COS-7 Nuclear Hypoxic Induced Cell Lysate [NB800-PC26] - WB analysis of 50ug nuclear lysate of COS7 cells which were treated with Cobalt Chloride / CoCl2 (Lane 1) or were left untreated (Lane 2) before preparation of lysates (catalog# NB800-PC26). The blot was developed using HIF-1 alpha antibody (catalog# NB100-479).](http://images.novusbio.com/fullsize/COS-7-Nuclear-Hypoxic-Induced-Cell-Lysate-Western-Blot-NB800-PC26-img0002.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0007.jpg "Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.")
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Ponceau S staining of HeLa whole cell lysate using NB800-PC1.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0003.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Analysis of STIM-1 antibody (NB110-60547) using HeLa whole cell lysate [NB800-PC1].](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0004.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml concentration. Detection was performed using ECL based method with 1 minute exposure time.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0005.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0006.jpg)
![Western Blot: Jurkat Whole Cell Lysate [NB800-PC2] - Western blot analysis of Actin (NB600-532) using Jurkat whole cell lysate using NB800-PC2.](http://images.novusbio.com/fullsize/Jurkat-Whole-Cell-Lysate-Western-Blot-NB800-PC2-img0003.jpg "Western Blot: Jurkat Whole Cell Lysate [NB800-PC2] - Western blot analysis of Actin (NB600-532) using Jurkat whole cell lysate using NB800-PC2.")
![SDS-Page: Jurkat Whole Cell Lysate [NB800-PC2] - Ponceau S staining of Jurkat whole cell lysate using NB800-PC2.](http://images.novusbio.com/fullsize/Jurkat-Whole-Cell-Lysate-SDS-Page-NB800-PC2-img0005.jpg)
![Western Blot: Raji Whole Cell Lysate [NB800-PC11] - Coommassie stain of 0.02 ml of NB 800-PC11 separated on a 4-20% gradient gel under reducing conditions (lane 2). MW standard is shown in lane 1.](http://images.novusbio.com/fullsize/Raji-Whole-Cell-Lysate-Western-Blot-NB800-PC11-img0001.jpg "Western Blot: Raji Whole Cell Lysate [NB800-PC11] - Coommassie stain of 0.02 ml of NB 800-PC11 separated on a 4-20% gradient gel under reducing conditions (lane 2). MW standard is shown in lane 1.")
![SDS-Page: Raji Whole Cell Lysate [NB800-PC11] - Coommassie stained SDS-PAGE of 20 ug of A 549 WCL, Raji WCL separated using a 4-20% gradient gel under reducing conditions. Molecular weight standards are shown.](http://images.novusbio.com/fullsize/Raji-Whole-Cell-Lysate-SDS-Page-NB800-PC11-img0002.jpg)
![Western Blot: NTERA-2 Whole Cell Lysate [NBP1-44968] - Analysis of GAPDH (NB300-323) using NTERA-2 whole cell lysate [NBP1-44968].](http://images.novusbio.com/fullsize/NTERA-2-Whole-Cell-Lysate-Western-Blot-NBP1-44968-img0004.jpg "Western Blot: NTERA-2 Whole Cell Lysate [NBP1-44968] - Analysis of GAPDH (NB300-323) using NTERA-2 whole cell lysate [NBP1-44968].")
![SDS-Page: NTERA-2 Whole Cell Lysate [NBP1-44968] - Staining of NTERA-2 whole cell lysate using NBP1-44968.](http://images.novusbio.com/fullsize/NTERA-2-Whole-Cell-Lysate-SDS-Page-NBP1-44968-img0003.jpg)
![Western Blot: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Western blot analysis of Beta Actin (NB600-532) using v6.5 Mouse embryonic stem cells Lysate (NBP1-97433).](http://images.novusbio.com/fullsize/v6.5-Mouse-embryonic-stem-cells-Whole-Cell-Lysate-Western-Blot-NBP1-97433-img0006.jpg "Western Blot: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Western blot analysis of Beta Actin (NB600-532) using v6.5 Mouse embryonic stem cells Lysate (NBP1-97433).")
![SDS-Page: v6.5 Mouse embryonic stem cells Whole Cell Lysate [NBP1-97433] - Ponceau Stain: Staining of v6.5 Mouse embryonic stem cells Lysate using NBP1-97433.](http://images.novusbio.com/fullsize/v6.5-Mouse-embryonic-stem-cells-Whole-Cell-Lysate-SDS-Page-NBP1-97433-img0004.jpg)
![Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stained SDS-PAGE of 40 ug of Human Derived A431 Whole Cell Lysate separated in a 4-20% gradient gel under non-reducing conditions.](http://images.novusbio.com/fullsize/A-431-Whole-Cell-Lysate-Western-Blot-NB800-PC12-img0002.jpg "Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stained SDS-PAGE of 40 ug of Human Derived A431 Whole Cell Lysate separated in a 4-20% gradient gel under non-reducing conditions.")
![Western Blot: A-431 Whole Cell Lysate [NB800-PC12] - Coommassie stain of 0.02 ml of NB 800-PC12 separated in a 4-20% gradient gel under non-reducing conditions (lane 1). MW standard is shown in lane 2.](http://images.novusbio.com/fullsize/A-431-Whole-Cell-Lysate-Western-Blot-NB800-PC12-img0001.jpg)