CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Nectin-4 was detected in immersion fixed paraffin-embedded sections of human placenta using 10 µg/mL Goat Anti-Human Nectin-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2659) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (F0107)." class="big_lightbox"> AB-108-C, open histogram), followed by PE-conjugated Anti-Goat IgG Secondary Antibody (F0107)." class="big_thumb" />

MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." class="big_lightbox"> MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." alt="U937 human histiocytic lymphoma cell line was stained with Human Nectin-1 Monoclonal Antibody (Catalog # MAB2880, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

3064-N3) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera (Catalog # 10287-N1) binds with an ED₅₀ of 2.5-20 ng/mL." class="big_lightbox"> 3064-N3) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera (Catalog # 10287-N1) binds with an ED₅₀ of 2.5-20 ng/mL." alt="When Recombinant Human Nectin-3 (Catalog # 3064-N3) is immobilized at 1 µg/mL (100 µL/well), Recombinant Cynomolgus Monkey/Rhesus Macaque Nectin-1 Fc Chimera (Catalog # 10287-N1) binds with an ED₅₀ of 2.5-20 ng/mL." class="big_thumb" />

3064-N3) is immobilized at 3.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Nectin‑1 His-tag Avi-tag Protein (Catalog # AVI2880) that produces 50% of the optimal binding response is 0.100-1.00 μg/mL" class="big_lightbox"> 3064-N3) is immobilized at 3.0 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human Nectin‑1 His-tag Avi-tag Protein (Catalog # AVI2880) that produces 50% of the optimal binding response is 0.100-1.00 μg/mL" class="big_thumb" />

3064-N3) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human Nectin‑1 Fc Chimera Avi-tag (Catalog # AVI10697) that produces 50% of the optimal binding response is approximately 6.0-40 ng/mL." class="big_lightbox"> 3064-N3) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human Nectin‑1 Fc Chimera Avi-tag (Catalog # AVI10697) that produces 50% of the optimal binding response is approximately 6.0-40 ng/mL." alt="When Recombinant Human Nectin-3 Protein (3064-N3) is immobilized at 0.5 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human Nectin‑1 Fc Chimera Avi-tag (Catalog # AVI10697) that produces 50% of the optimal binding response is approximately 6.0-40 ng/mL." class="big_thumb" />

3064-N3) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Nectin-1 Fc Chimera (Catalog # 10697-N1) binds with an ED₅₀ of 2.5‑20 ng/mL." class="big_lightbox"> 3064-N3) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Nectin-1 Fc Chimera (Catalog # 10697-N1) binds with an ED₅₀ of 2.5‑20 ng/mL." alt="When Recombinant Human Nectin-3 (3064-N3) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human Nectin-1 Fc Chimera (Catalog # 10697-N1) binds with an ED₅₀ of 2.5‑20 ng/mL." class="big_thumb" />