Cytokeratin 14 Antibodies
We offer Cytokeratin 14 Antibodies for use in common research applications: ELISA, Flow Cytometry, Immunocytochemistry, Immunocytochemistry/Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot. Each Cytokeratin 14 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our Cytokeratin 14 Antibodies can be used in a variety of model species: Canine, Feline, Human, Mouse, Porcine, Rat. Use the list below to choose the Cytokeratin 14 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our Cytokeratin 14 polyclonal antibodies and browse our Cytokeratin 14 monoclonal antibody catalog.
Alternate Names for Cytokeratin 14 Antibodies
anti-Cytokeratin 14 antibody, anti-KRT14 antibody, anti-CK-14 antibody, anti-CK14 antibody, anti-cytokeratin-14 antibody, anti-EBS3 antibody, anti-EBS4 antibody, anti-K14 antibody, anti-keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody, anti-keratin 14 antibody, anti-keratin, type I cytoskeletal 14 antibody, anti-keratin-14 antibody, anti-NFJ antibody
55 Results for "cytokeratin-14" in Primary Antibodies
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Immunocytochemistry-Immunofluorescence-NBP2-34270-img0010.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Immunohistochemistry-Paraffin-NBP2-34270-img0011.jpg)
![Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Flow-Cytometry-NBP2-34270-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate stained with Cytokeratin 14 Monoclonal Antibody (LL002)](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Immunohistochemistry-Paraffin-NBP2-34270-img0006.jpg)
![Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Simple Western lane view shows a specific band for Cytokeratin 14 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Simple-Western-NBP2-34270-img0004.jpg)
![Simple Western: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 14 antibody was used at 10 ug/ml dilution of A431 lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-LL002-Simple-Western-NBP2-34270-img0005.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0008.jpg "Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Western blot analysis of Cytokeratin 14 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/400 dilution and Vimentin (green) at 1/400 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) and Goat Anti-Mouse IgG H&L (iFluor(TM) 488) were used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0010.jpg)
![Western Blot: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of Cytokeratin 14 on different lysates using anti-Cytokeratin 14 antibody at 1/1,000 dilution. Positive control: Lane 1: A431 Lane 2: Human skin](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Western-Blot-NBP2-67585-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Staining Cytokeratin 14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunocytochemistry-Immunofluorescence-NBP2-67585-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Analysis of paraffin-embedded mouse skin tissue using anti-Cytokeratin 14 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SC65-06) [NBP2-67585] - Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (red) at 1/500 dilution at +4 overnight, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 594) was used as the secondary antibodies at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SC65-06-Immunohistochemistry-Paraffin-NBP2-67585-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Immunofluorescence Analysis of A549 cells labeling KRT14 with Cytokeratin 14 Antibody (SPM263) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SPM263-Immunocytochemistry-Immunofluorescence-NBP2-34355-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Immunofluorescence Analysis of A549 cells labeling KRT14 with Cytokeratin 14 Antibody (SPM263) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Formalin-paraffin prostate (20X) stained with Cytokeratin 14 MAb (SPM263).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SPM263-Immunohistochemistry-Paraffin-NBP2-34355-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Formalin-paraffin prostate (10X) stained with Cytokeratin 14 MAb (SPM263).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-SPM263-Immunohistochemistry-Paraffin-NBP2-34355-img0001.jpg)
![Flow Cytometry: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Enrichment of Cytokeratin 14 (high cells after UTX knockdown in HBLAK cells. Cytokeratin 14 expression as detected by flow cytometry on day 4 after transfection of control siRNA or UTX-siRNA (n = 4). Statistics were performed with a two-way ANOVA with a post-hoc Tukey HSD test (## p < 0.01; ### p < 0.001). Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/12/4/1023/htm), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-34355_mouse-monoclonal-cytokeratin-14-antibody-spm263-1772024936269.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) [NBP2-34355] - Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (SPM263) - Human Tonsil tissue with epithelial CK14 expression as monochromatic image. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-34355_mouse-monoclonal-cytokeratin-14-antibody-spm263-17720241003233.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31326-img0009.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody [NBP1-31326] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 14 protein stained by Cytokeratin 14 antibody diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-31326] - Lung CA, using Cytokeratin 14 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-31326-img0004.jpg)
![Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000.](http://images.novusbio.com/fullsize/nbp1-31326_rabbit-polyclonal-cytokeratin-14-antibody-91020242013123.jpg)
![Western Blot: Cytokeratin 14 Antibody [NBP1-31326] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 14 antibody (NBP1-31326) diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31326_rabbit-polyclonal-cytokeratin-14-antibody-9102024195726.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-2375-Immunohistochemistry-Paraffin-NBP3-07600-img0004.jpg "Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).")
![Flow Cytometry: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 14 Mouse Monoclonal Antibody ((KRT14/2375) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-2375-Flow-Cytometry-NBP3-07600-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-2375-Immunohistochemistry-Paraffin-NBP3-07600-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/2375) [NBP3-07600] - Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 14 Mouse Monoclonal Antibody (KRT14/2375).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-2375-Immunohistochemistry-Paraffin-NBP3-07600-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/532) [NBP2-45032] - Human Tonsil stained with Cytokeratin 14 Monoclonal Antibody (KRT14/532).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-532-Immunohistochemistry-Paraffin-NBP2-45032-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/532) [NBP2-45032] - Human Tonsil stained with Cytokeratin 14 Monoclonal Antibody (KRT14/532).")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (KRT14/532) [NBP2-45032] - Human Cervix stained with Cytokeratin 14 Monoclonal Antibody (KRT14/532).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-KRT14-532-Immunohistochemistry-Paraffin-NBP2-45032-img0001.jpg)
![Western Blot: Cytokeratin 14 Antibody [NBP1-84917] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Western-Blot-NBP1-84917-img0005.jpg "Western Blot: Cytokeratin 14 Antibody [NBP1-84917] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-84917] - Staining in human skin and duodenum tissues using anti-KRT14 antibody. Corresponding KRT14 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-84917-img0011.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-84917] - Staining of human duodenum shows low expression as expected.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-84917-img0009.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody [NBP1-84917] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-Immunohistochemistry-Paraffin-NBP1-84917-img0010.jpg)
![Western Blot: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDa Observed band size:52 kDa](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Western-Blot-NBP2-89198-img0003.jpg "Western Blot: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDa Observed band size:52 kDa")
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198] - Immunochemical staining of human Cytokeratin14 in human esophageal carcinoma with rabbit monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89198-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (101) [NBP2-89198] - Staining of human CK14 in human skin with rabbit monoclonal antibody (1:20000).](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Immunohistochemistry-Paraffin-NBP2-89198-img0001.jpg)
![Immunoprecipitation: Cytokeratin 14 Antibody (101) [NBP2-89198] - Lane A:0.5 mg A431 Whole Cell Lysate 2 uL anti-KRT14 rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody:Anti-KRT14 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilutionDeveloped using the DAB staining technique. Performed under reducing conditions. Predicted band size: 52 kDa Observed band size: 52 kDa](http://images.novusbio.com/fullsize/Cytokeratin-14-Antibody-101-Immunoprecipitation-NBP2-89198-img0002.jpg)
![Western Blot: Cytokeratin 14 Antibody (A2C11-R) [NBP3-32242] - Western blot analysis of Cytokeratin 14 on different lysates with Mouse anti-Cytokeratin 14 antibody (NBP3-32242) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (negative) (20 ug/Lane) Lane 3: PANC-1 cell lysate (negative) (20 ug/Lane) Lane 4: Mouse skin tissue lysate (40 ug/Lane) Lane 5: Rat skin tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32242) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32242_mouse-cytokeratin-14-mab-a2c11-r-278202415295571.jpg "Western Blot: Cytokeratin 14 Antibody (A2C11-R) [NBP3-32242] - Western blot analysis of Cytokeratin 14 on different lysates with Mouse anti-Cytokeratin 14 antibody (NBP3-32242) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: MCF7 cell lysate (negative) (20 ug/Lane) Lane 3: PANC-1 cell lysate (negative) (20 ug/Lane) Lane 4: Mouse skin tissue lysate (40 ug/Lane) Lane 5: Rat skin tissue lysate (40 ug/Lane) Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32242) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 14 Antibody (A2C11-R) [NBP3-32242] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Cytokeratin 14 antibody (NBP3-32242) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32242) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32242_mouse-cytokeratin-14-mab-a2c11-r-29820241435249.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 14 Antibody (A2C11-R) [NBP3-32242] - Immunocytochemistry analysis of A431 (positive) and PANC-1 (negative) labeling Cytokeratin 14 with Rabbit anti-Cytokeratin 14 antibody (NBP3-32242) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 14 antibody (NBP3-32242) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32242_mouse-cytokeratin-14-mab-a2c11-r-29202410484820.jpg)