| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70454 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97494-0.2mg | |||
| BSA Free | NBP1-97494-0.025mg | |||
HAF007). For additional reference, recombinant human AlphaB Crystallin/CRYAB and recombinant human AlphaA Crystallin/CRYAA (5 ng/lane) were included. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat heart tissue, mouse heart tissue, and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Monoclonal Antibody (Catalog # MAB4849) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human AlphaB Crystallin/CRYAB and recombinant human AlphaA Crystallin/CRYAA (5 ng/lane) were included. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4849-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-13497-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4849-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44221 | |||
| BSA Free | NBP3-07986-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49246-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08380 | |||
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Western-Blot-NBP1-47708-img0008.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-Crystallin AB monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Crystallin AB.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunocytochemistry-Immunofluorescence-NBP1-47708-img0011.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human pancreas tissue using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunohistochemistry-Paraffin-NBP1-47708-img0013.jpg)
![Flow Cytometry: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Crystallin AB antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Flow-Cytometry-NBP1-47708-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of HT29 cells using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunocytochemistry-Immunofluorescence-NBP1-47708-img0010.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - Staining of paraffin-embedded Human endometrium tissue using anti-Crystallin AB mouse monoclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunohistochemistry-Paraffin-NBP1-47708-img0012.jpg)
![Immunoprecipitation: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - (Negative control: IP without adding anti-CRYAB antibody.). For each experiment, 500ul of DDK tagged CRYAB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-CRYAB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-OTI6A9-Immunoprecipitation-NBP1-47708-img0014.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (OTI6A9) [NBP1-47708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CRYAB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with AlphaB Crystallin/CRYAB Antibody (OTI6A9).](http://images.novusbio.com/fullsize/nbp1-47708_mouse-alphab-crystallin-cryab-mab-oti6a9-17720241044629.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.](http://images.novusbio.com/fullsize/Crystallin-AB-Antibody-1B6-1-3G4-Western-Blot-NBP1-97494-img0004.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.")
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Human Heart tissue stained with alpha B-Crystallin, mAb (1B6.1-3G4) at 10ug/ml.](http://images.novusbio.com/fullsize/Crystallin-AB-Antibody-1B6-1-3G4-Immunohistochemistry-Paraffin-NBP1-97494-img0003.jpg)
![SDS-Page: Recombinant Human AlphaB Crystallin/CRYAB Protein [NBC1-18352] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain](http://images.novusbio.com/fullsize/Recombinant-Human-AlphaB-Crystallin-CRYAB-Protein-SDS-Page-NBC1-18352-img0001.jpg "SDS-Page: Recombinant Human AlphaB Crystallin/CRYAB Protein [NBC1-18352] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain")
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0009.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Rat Brain cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - analysis using Rabbit Anti-Alpha B Crystallin Polyclonal Antibody . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 20x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0002.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Western blot analysis of Human A431, HCT116, HeLa, HepG2, HEK293, HUVEC, Jurkat, MCF7, PC3 and T98G cell lysates showing detection of ~22 kDa AlphaB Crystallin/CRYAB protein using Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497). Lane 1: Molecular Weight Ladder (MW). Lane 2: A431 cell lysates. Lane 3: HCT116 cell lysates. Lane 4: HeLa cell lysates. Lane 5: HepG2 cell lysates. Lane 6: HEK293 cell lysates. Lane 7: HUVEC cell lysates. Lane 8: Jurkat cell lysates. Lane 9: MCF7 cell lysates. Lane 10: PC3 cell lysates. Lane 11: T98G cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AlphaB Crystallin/CRYAB Polyclonal Antibody (NB120-13497) at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~22 kDa.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB120-13497-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB120-13497] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Alpha B Crystallin Polyclonal Antibody at 1:120 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Actin filament bundles. Nuclear splicing speckles. Exosomes. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB120-13497-img0003.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cerebral cortex and liver tissues using NBP3-07986 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cerebral cortex and liver tissues using NBP3-07986 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Analysis in human cell line U-2 OS.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Western-Blot-NBP3-07986-img0008.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human cerebral cortex shows strong cytoplasmic positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0003.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0004.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of human rectum shows strong cytoplasmic positivity in peripheral ganglia and nerves.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0005.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CL10758) [NBP3-07986] - Staining of rat brain shows strong cytoplasmic positivity in oligodendrocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CL10758-Immunohistochemistry-Paraffin-NBP3-07986-img0006.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Analysis in human heart muscle and liver tissues using NBP2-49246 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0002.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Analysis in human heart muscle and liver tissues using NBP2-49246 antibody. Corresponding CRYAB RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0006.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Staining of human heart muscle shows strong cytoplasmic positivity in cardiomyocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0003.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Staining of human skeletal muscle shows moderate ctyoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0004.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody [NBP2-49246] - Staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunohistochemistry-Paraffin-NBP2-49246-img0005.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Western Blot Analysis of HeLa cell lysate using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Western-Blot-NBP3-07764-img0004.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Western Blot Analysis of HeLa cell lysate using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).")
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded Rat Heart Muscle stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Immunohistochemistry-Paraffin-NBP3-07764-img0003.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded human Breast stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Immunohistochemistry-Paraffin-NBP3-07764-img0002.jpg)
![Protein Array: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Analysis of Protein Array containing more than 19,000 full-length human proteins using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CRYAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Protein-Array-NBP3-07764-img0001.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Western-Blot-NBP3-16844-img0003.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Western blot analysis of extracts of various cell lines, using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunofluorescence analysis of C6 cells using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunocytochemistry-Immunofluorescence-NBP3-16844-img0002.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (6C9R1) [NBP3-16844] - Immunohistochemistry of paraffin-embedded rat kidney using AlphaB Crystallin/CRYAB Rabbit mAb (NBP3-16844) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-6C9R1-Immunohistochemistry-Paraffin-NBP3-16844-img0001.jpg)
