Product Search for s1p1 edg 1: Novus Biologicals

MAB7089

S1P1/EDG-1 Antibody (713412) [Unconjugated]

100 μg

MAB7089

MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

HEK293 human embryonic kidney cell line transfected with mouse S1P<sub>1</sub>/EDG-1 and GFP was stained with (A) Rat Anti-Mouse S1P<sub>1</sub>/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # <a class=

MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." alt="HEK293 human embryonic kidney cell line transfected with mouse S1P₁/EDG-1 and GFP was stained with (A) Rat Anti-Mouse S1P₁/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # <a class=

FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

S1P<SUB>1</SUB>/EDG-1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P<SUB>1</SUB>/EDG-1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

CD69 and S1PR1 expression on CD4 T cells before and following cell transfer. Activated OT-II T cells were analyzed before cell transfer (A) for expression of CD69 and S1PR1 or for CD69 expression ex vivo 24 h after transfer into resting (PBS) or inflamed [lipopolysaccharide (LPS)] ear pinnae (B,C). Before transfer, the OT-II T cells were treated with an S1PR1 antagonist or agonist as in Figure 5B. In panel (A), cells are gated on live CD4+ CD45+ cells; representative of three independent samples. In panels (B,C), data are combined from two to three independent experiments with ≥3 animals per group. In this figure, p < 0.05 is indicated by * and p < 0.001 by **. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/29225602), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB7089U-100UG
Alexa Fluor 405	FAB7089V-100UG
Alexa Fluor 488	FAB7089G-100ug
Alexa Fluor 532	FAB7089X
Alexa Fluor 594	FAB7089T-100UG
Alexa Fluor 647	FAB7089R-100UG
Alexa Fluor 700	FAB7089N
Alexa Fluor 750	FAB7089S-100UG
Alexa Fluor Plus 405	FAB7089AFP405-100UG
Alexa Fluor Plus 488	FAB7089AFP488-100UG
Alexa Fluor Plus 555	FAB7089AFP555-100UG
Alexa Fluor Plus 594	FAB7089AFP594-100UG
Alexa Fluor Plus 647	FAB7089AFP647-100UG
Alexa Fluor Plus 680	FAB7089AFP680-100UG
Allophycocyanin	FAB7089A-100UG
Allophycocyanin/Cy7	FAB7089APCCY7
Biotin	FAB7089B
CoraFluor 1	FAB7089CL1
DyLight 350	FAB7089D
DyLight 405	FAB7089E
DyLight 488	FAB7089K
DyLight 550	FAB7089L
DyLight 594	FAB7089M
DyLight 650	FAB7089W
DyLight 680	FAB7089Y
DyLight 755	FAB7089Z
FITC	FAB7089F
HRP	FAB7089H
Janelia Fluor 525	FAB7089JF525
Janelia Fluor 549	FAB7089I
Janelia Fluor 646	FAB7089J
Janelia Fluor 669	FAB7089JF669
PE/Atto594	FAB7089PEATT594
PE/Cy5.5	FAB7089PECY55
PE/Cy7	FAB7089PECY7
PerCP	FAB7089C
Phycoerythrin	FAB7089P-100UG
mFluor Violet 450 SE	FAB7089MFV450
mFluor Violet 500 SE	FAB7089MFV500
mFluor Violet 610 SE	FAB7089MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB7089B
Carrier Free	FAB7089N
Carrier Free	MAB7089-SP
Carrier Free	FAB7089V-100UG
Carrier Free	FAB7089U-100UG
Carrier Free	FAB7089T-100UG
Carrier Free	FAB7089S-100UG
Carrier Free	FAB7089R-100UG
Carrier Free	FAB7089G-100ug
Carrier Free	FAB7089P-100UG
Carrier Free	FAB7089A-100UG

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NBP2-67129

S1P1/EDG-1 Antibody (JM10-66)

100 ul

NBP2-67129

Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.

Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB2016

S1P1/EDG-1 Antibody (218713) [Unconjugated]

(1 Review)

(12 Publications)

100 μg

MAB2016

F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox">

CHO Chinese hamster ovary cell line transfected with either (A) human S1P<sub>1</sub>/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P<sub>1</sub>/EDG-1 Monoclonal Antibody (Catalog # MAB2016) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." alt="CHO Chinese hamster ovary cell line transfected with either (A) human S1P₁/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P₁/EDG-1 Monoclonal Antibody (Catalog # MAB2016) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

<b>Biological Strategies Validation. </b>S1PR1 expression by OECs is dynamically altered by microenvironment.OEC expression of S1PR1 under normoxia and hypoxia was qualitatively confirmed by immunocytochemistry (A). The percentage of cells expressing S1PR1 on the cell surface, as confirmed with flow cytometry, was enhanced under hypoxia as compared to the normoxic control (B). Combined stimuli of S1P and VEGF resulted in greater S1PR1 mRNA production after 24h in normoxia (C). Combined stimuli also resulted in a greater proportion of S1PR1+ OECs under both normoxia and hypoxia (D and E). Scale bars represent 40 μm. Data are mean ± SD (n = 4) and normalized to the average untreated control values for either normoxia or hypoxia (C, E; indicated by dashed line) accordingly. An asterisk indicates statistically significant differences (P<0.05) between conditions in normoxia or hypoxia respectively and N.S. displays no statistically significant difference between conditions. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0123437), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2016U-100UG
Alexa Fluor 405	FAB2016V-100UG
Alexa Fluor 488	FAB2016G-100UG
Alexa Fluor 532	FAB2016X
Alexa Fluor 594	FAB2016T-100UG
Alexa Fluor 647	FAB2016R-100UG
Alexa Fluor 700	FAB2016N
Alexa Fluor 750	FAB2016S-100UG
Alexa Fluor Plus 405	FAB2016AFP405-100UG
Alexa Fluor Plus 488	FAB2016AFP488-100UG
Alexa Fluor Plus 555	FAB2016AFP555-100UG
Alexa Fluor Plus 594	FAB2016AFP594-100UG
Alexa Fluor Plus 647	FAB2016AFP647-100UG
Alexa Fluor Plus 680	FAB2016AFP680-100UG
Allophycocyanin	FAB2016A
Allophycocyanin/Cy7	FAB2016APCCY7
Biotin	FAB2016B
CoraFluor 1	FAB2016CL1
DyLight 350	FAB2016D
DyLight 405	FAB2016E
DyLight 488	FAB2016K
DyLight 550	FAB2016L
DyLight 594	FAB2016M
DyLight 650	FAB2016W
DyLight 680	FAB2016Y
DyLight 755	FAB2016Z
FITC	FAB2016F
HRP	FAB2016H
Janelia Fluor 525	FAB2016JF525
Janelia Fluor 549	FAB2016I
Janelia Fluor 646	FAB2016J
Janelia Fluor 669	FAB2016JF669
PE/Atto594	FAB2016PEATT594
PE/Cy5.5	FAB2016PECY55
PE/Cy7	FAB2016PECY7
PerCP	FAB2016C
Phycoerythrin	FAB2016P
mFluor Violet 450 SE	FAB2016MFV450
mFluor Violet 500 SE	FAB2016MFV500
mFluor Violet 610 SE	FAB2016MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2016P
Carrier Free	FAB2016A
Carrier Free	FAB2016N
Carrier Free	MAB2016-SP
Carrier Free	MAB2016-500
Carrier Free	FAB2016G-100UG
Carrier Free	FAB2016V-100UG
Carrier Free	FAB2016U-100UG
Carrier Free	FAB2016T-100UG
Carrier Free	FAB2016S-100UG
Carrier Free	FAB2016R-100UG

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NB120-11424

S1P1/EDG-1 Antibody

(5 Publications)

100 μg

NB120-11424

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Analysis of S1P1 from transfected HEK293 cell lysate.

Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody [NB120-11424] - Immunofluorescence staining of S1P1 in transfected CCL39 cells using NB120-11424.

<b>Biological Strategies Validation. </b>S1P1-EDG-1-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11424-img0009.jpg

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Gene & protein expression of components of the PAR-1/SphK/S1P axis. qRT-PCR analysis of the gene expression of (A) PAR-1, (B) S1PR1, (C) SphK1, & (D) SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for 0 h, 1 h, or 6 h. Western blot detection & quantification of the protein expression of PAR-1, S1PR1, SphK1, & SphK2 in astrocytes treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only), for (E) 0 h, (F) 1 h, or (G) 6 h. The data are presented as the mean ± SD (n = 3), *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: S1P1/EDG-1 Antibody [NB120-11424] - Expression of sphingosine-1-phosphate (S1P), interleukin (IL)-1 beta , & protease-activated receptor-1 (PAR-1) in isolated astrocytes. (A) Isolated & purified astrocytes were identified by bright-field microscopy & immunofluorescence for glial fibrillary acid protein (GFAP; red). Nuclei (DAPI) were labeled in blue. Scale bar = 100 μm. (B) Western blot & quantitative reverse transcription polymerase chain reaction (qRT-PCR) of the protein & mRNA expression, respectively, of S1P 0, 1, & 6 h after astrocytes were treated with lipopolysaccharide (LPS) or thrombin & Dabigatran (Dab) or PAR-1-inh (LPS only). (D) qRT-PCR & (E) enzyme-linked immunosorbent assay (ELISA) of the mRNA expression & secretion, respectively, of IL-1 beta 0, 1, & 6 h after astrocytes were treated with LPS or thrombin & Dab or PAR-1-inh (LPS only). (F) Immunofluorescence of astrocytes & quantification of relative mean integrated optical density (IOD; green fluorescence). PAR-1 was stained in green & nuclei (DAPI) were stained in blue. Scale bar = 100 μm. For (B–F), data are presented as the mean ± SD (n = 3). *p < 0.05; #p < 0.05 vs. the same group at 0 h; &p < 0.05 vs. the same group at 1 h. ns, not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunofluorescence of proteins related to S1P signaling in astrocytes. Astrocytes were treated with LPS or thrombin, with or without Dab or PAR-1-inh (LPS only) for 6 h. Cells were stained for (A) S1P receptor 1 (S1PR1), (B) sphingosine kinase (SphK1), and (C) SphK2 in green and nuclei (DAPI) in blue. Scale bar = 100 μm. Green fluorescence was quantified by measuring the relative mean IOD. The data are presented as the mean +/- SD (n = 3), *p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32694981), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP3-16311

S1P1/EDG-1 Antibody (9R8N3)

100 ul

NBP3-16311

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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H00001901-Q01

Recombinant Human S1P1/EDG-1 GST (N-Term) Protein

10 μg

H00001901-Q01

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

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NBP2-82426

Mouse S1P1/EDG-1 ELISA Kit (Colorimetric)

1 Kit

NBP2-82426

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

0.16 - 10 ng/mL

Sensitivity:

0.09 ng/mL

Sample Type:

Serum, Plasma, Urine, Cell Supernatant, Tissue Homogenate

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NBP2-58024

S1P1/EDG-1 Antibody - BSA Free

100 ul

NBP2-58024

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-58024-25ul

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NBP3-15216

S1P1/EDG-1 Antibody (4F2) - BSA Free

100 ul

NBP3-15216

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-52541

Human S1P1/EDG-1 - Ready-To-Use ELISA Kit (Colorimetric)

1 Kit

NBP3-52541

ELISA Human S1P1/EDG-1 - Ready-To-Use ELISA Kit (Colorimetric)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.156 - 10 ng/mL (example only; lot dependent)

Sensitivity:

0.063 ng/mL (example only; lot dependent)

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

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NBP3-55799

Human S1P1/EDG-1 ELISA Kit (Colorimetric)

1 Kit

NBP3-55799

ELISA Human S1P1/EDG-1 ELISA Kit (Colorimetric)

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.156 - 10 ng/mL (example only; lot dependent)

Sensitivity:

0.063 ng/mL (example only; lot dependent)

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

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NBP2-58024PEP

S1P1/EDG-1 Recombinant Protein Antigen

100 ul

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

S1P1/EDG-1 Antibody (713412) [Unconjugated]

S1P1/EDG-1 Antibody (JM10-66)

S1P1/EDG-1 Antibody (218713) [Unconjugated]

S1P1/EDG-1 Antibody

S1P1/EDG-1 Antibody (9R8N3)

Recombinant Human S1P1/EDG-1 GST (N-Term) Protein

Mouse S1P1/EDG-1 ELISA Kit (Colorimetric)

S1P1/EDG-1 Antibody - BSA Free

S1P1/EDG-1 Antibody (4F2) - BSA Free

Human S1P1/EDG-1 - Ready-To-Use ELISA Kit (Colorimetric)

Human S1P1/EDG-1 ELISA Kit (Colorimetric)

S1P1/EDG-1 Recombinant Protein Antigen