| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32035-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90174-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61137 | |||
| BSA Free | NB300-146SS | |||
| Azide and BSA Free | NBP2-80956 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38595-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92591-0.02ml | |||
![H+/K+ ATPase Activity Assay Kit (Colorimetric) [NBP3-25817] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-25817_h-k-atpase-activity-assay-kit-colorimetric-812024943296.png "H+/K+ ATPase Activity Assay Kit (Colorimetric) [NBP3-25817] - Standard Curve Reference")
![H+/K+ ATPase Activity Assay Kit (Colorimetric) [NBP3-25817] - Assay Example](http://images.novusbio.com/fullsize/nbp3-25817_h-k-atpase-activity-assay-kit-colorimetric-8120241131133.png)
![Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-583-img0010.jpg "Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.")
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human stomach tissues.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunohistochemistry-Paraffin-NB300-583-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-583-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Hydrogen/Potassium ATPase beta staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Hydrogen/Potassium ATPase beta at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-583-img0009.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human gastric carcinoma tissues.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunohistochemistry-Paraffin-NB300-583-img0006.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody (2G11) [NB300-583] - Both normal and cancer biopsies of deparaffinized Human gastric carcinoma tissues.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-2G11-Immunohistochemistry-Paraffin-NB300-583-img0012.jpg)
![Western Blot: ATPase Na+/K+ beta 3 Antibody [H00000483-B01P] - Analysis of ATP1B3 expression in A-431.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-Western-Blot-H00000483-B01P-img0005.jpg "Western Blot: ATPase Na+/K+ beta 3 Antibody [H00000483-B01P] - Analysis of ATP1B3 expression in A-431.")
![Flow Cytometry: ATPase Na+/K+ beta 3 Antibody [H00000483-B01P] - Analysis of negative control 293 cells (Black) and ATP1B3 expressing 293 cells (Green) using ATP1B3 purified mouse polyclonal antibody.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-Flow-Cytometry-H00000483-B01P-img0004.jpg)
![Western Blot: ATPase Na+/K+ beta 3 Antibody [H00000483-B01P] - Analysis of ATP1B3 expression in transfected 293T cell line by ATP1B3 polyclonal antibody. Lane 1: ATP1B3 transfected lysate(30.69 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-Western-Blot-H00000483-B01P-img0006.jpg)
![Immunohistochemistry: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human stomach, lower shows strong cytoplasmic positivity in subset of glandular cells.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-NBP2-32035-img0004.jpg "Immunohistochemistry: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human stomach, lower shows strong cytoplasmic positivity in subset of glandular cells.")
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0005.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human stomach shows high expression.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining in human stomach and pancreas tissues using anti-ATP4B antibody. Corresponding ATP4B RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human colon, kidney, liver and stomach using Anti-ATP4B antibody NBP2-32035 (A) shows similar protein distribution across tissues to independent antibody NBP2-38674 (B).](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0008.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human kidney.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0009.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human liver.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0010.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035] - Staining of human colon.](http://images.novusbio.com/fullsize/Hydrogen-Potassium-ATPase-Beta-Antibody-Immunohistochemistry-Paraffin-NBP2-32035-img0011.jpg)
![Immunohistochemistry-Paraffin: Hydrogen Potassium ATPase Beta Antibody [NBP2-32035]-Positive staining can be observed in human gastric parietal cells using this antibody. Red is Hydrogen Potassium ATPase Beta. This antibody is very specific.](http://images.novusbio.com/fullsize/antibody/nbp2-32035_rabbit-polyclonal-hydrogen-potassium-atpase-beta-antibody-immunohistochemistry-paraffin-254202391250.jpg)
![ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05] - Detection limit for recombinant GST tagged ATP1B3 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-2F4-ELISA-H00000483-M05-img0001.jpg "ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05] - Detection limit for recombinant GST tagged ATP1B3 is approximately 3ng/ml as a capture antibody.")
![ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05] - Detection limit for recombinant GST tagged ATP1B3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-2F4-ELISA-H00000483-M05-img0002.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Transfected SK-N-AS cells express ICAM-2 transcripts and proteins. ICAM-2 WT and variants localized to cell membranes. Experimental details are included in Methods. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-261), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Western-Blot-NB300-146-img0022.jpg "Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Transfected SK-N-AS cells express ICAM-2 transcripts and proteins. ICAM-2 WT and variants localized to cell membranes. Experimental details are included in Methods. Image collected and cropped by CiteAb from the following publication (//bmccancer.biomedcentral.com/articles/10.1186/1471-2407-13-261), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier. Immunofluorescent staining of Na+K+ ATPase and ABCC1, showing the expected respective apical and basolateral membrane localization in the choroidal epithelial cells. The left image is a close up of a single cell to better appreciate the polarity of distribution of the 2 proteins. Nuclei appear in blue. Arrows show the lateral cellular membranes best seen in the z direction by confocal analysis, arrowheads show the basal labeling of ABCC1. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0023.jpg)
![Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Staining of human enodmetrial glands within the uterus using NB300-146. Note the absence of staining in the surrounding myometrial smooth muscle.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunohistochemistry-NB300-146-img0012.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on NIH3T3 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0027.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Western-Blot-NB300-146-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Hek293 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Sodium Potassium ATPase Alpha 1 (464.6) at 10 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Detection of ATPA1 (Green) in HepG2 cells using NB300-146. Nuclei (Blue) were counterstained using Hoechst 33258.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier. Immunofluorescent staining of Na+K+ ATPase and ABCC1, showing the expected respective apical and basolateral membrane localization in the choroidal epithelial cells. The left image is a close up of a single cell to better appreciate the polarity of distribution of the 2 proteins. Nuclei appear in blue. Arrows show the lateral cellular membranes best seen in the z direction by confocal analysis, arrowheads show the basal labeling of ABCC1. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150945), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured up to 4 weeks on transwell inserts covered with laminin. The expression of Na+, K+-ATPase using anti-alpha1 antibody was observed mostly at the basolateral membrane. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphys.2016.00450/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Immunocytochemistry-Immunofluorescence-NB300-146-img0020.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with NB300-146 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0016.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with NBP2-61137AF647 (Blue) and a matched isotype control (Orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0017.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on A549 cells with - Purified NBP2-61137 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems). Image using the Purified form of this antibody.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0018.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on RCC4 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0025.jpg)
![Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - An intracellular stain was performed on SK-MEL-28 cells with Sodium Potassium ATPase Alpha 1 Antibody (464.6) NB300-146 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-Flow-Cytometry-NB300-146-img0026.jpg)
![Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured up to 4 weeks on transwell inserts covered with laminin. The expression of Na+, K+-ATPase using anti-alpha1 antibody was observed mostly at the basolateral membrane. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphys.2016.00450/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Alpha-1-Antibody-464-6-NB300-146-img0024.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - The cellular model of the blood-CSF barrier.(A) Schematic representation of a choroidal villus & of the experimental set up illustrating the two-chamber culture device. Left: the choroidal epithelium which forms the actual tight barrier controlling access into the CSF [18] delimits a stroma, in which the fenestrated vessels lacking a typical blood-brain barrier phenotype, express P-selectin [4]. Right: The epithelial cell monolayer grown on the lower side of the filter separates the upper chamber corresponding to the blood/stromal or basolateral space, from the bottom chamber representing the CSF or apical compartment (adapted from [18,41]. (B & C) Immunofluorescent staining of occludin (B) & claudin 1/3 (C) showing a typical intercellular distribution of the tight junction proteins in the confluent inverted monolayers of choroidal epithelial cells. (D) Immunofluorescent staining of Na+K+ ATPase & ABCC1, showing the expected respective apical & basolateral membrane localization in the choroidal epithelial cells. The left image is a close up of a single cell to better appreciate the polarity of distribution of the 2 proteins. Nuclei appear in blue. Arrows show the lateral cellular membranes best seen in the z direction by confocal analysis, arrowheads show the basal labeling of ABCC1. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0150945), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-146_mouse-monoclonal-sodium-potassium-atpase-alpha-1-antibody-464-6-310202416205173.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - Endothelial-mesenchymal transformation (EMT) of RCECs using specific endothelial-mesenchymal transformation medium (SEMTM). (A) Immunohistochemistry of the fibroblastic markers alpha -SMA & vimentin were characteristic of fibroblasts in EMT-RCECs compared to the hexogonal morphology of Fresh RCECs (Scale bar upper panel = 100 µm, Scale bar middle & bottom panel = 50 µm). (B) RT-PCR showed that both Fresh RCECs & EMT-RCECs expressed alpha -Sma. Original gel is shown in Supplemental Fig. S2. However, qRT-PCR in the lower panel shows alpha -Sma was significantly upregulated in EMT-RCECs. (C) Western blots showed that Fresh RCECs & EMT-RCECs expressed Vimentin, Atp1a1, a marker of differentiated endothelial cells, & b-actin. Original gel & blots are shown in Supplemental Figs S3, 4. Semiquantitative analysis shows significant upregulation of the protein levels of Vimentin (p = 0.048) & downregulation of the protein level of Atp1a1 (p = 0.0043). Data expressed as mean ± SD of three replicate experiments. Student’s t test (*p < 0.05, **p < 0.01). (n = 3). RCECs: rabbit corneal endothelial cells, alpha -SMA: smooth muscle alpha -actin. Atp1a1: Na,K‐ATPase alpha ‐subunit. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30442918), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-146_mouse-monoclonal-sodium-potassium-atpase-alpha-1-antibody-464-6-310202416205123.jpg)
![Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - SDS–PAGE western blot validation of the increased abundance of Annexin V, Na/K ATPase & alpha -tubulin in the EVs derived from TIS Cal51 cells compared with EVs derived from non-senescent non-treated controls. EVs isolated from TIS & control cells were immunoblotted to validate the protein expression of annexin V, Na/K ATPase & alpha -tubulin from the MS data. CD63 & HSP70 were used as loading controls. Densitometry was completed using Image J software (Fiji). All values are expressed as the mean of three independent experiments±s.e.m. The limit of ±1 skewness was set as normal distribution. Skewed data were transformed to Y=Log(Y) to fit a normal distribution. Results were analysed using the Student’s t-test with significant differences having a *P⩽0.05, **P⩽0.01, ***P⩽0.001. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/oncsis201782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-146_mouse-monoclonal-sodium-potassium-atpase-alpha-1-antibody-464-6-310202416203713.jpg)
![Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) [NB300-146] - ARPE-19 cells cultured on transwell inserts for 4 weeks are not completely polarized. ARPE-19 cells were cultured up to 4 weeks on transwell inserts covered with laminin. The immunofluorescence image in (A) shows actin localization using rhodamine phalloidin. The cells are flat with stress fibers & very little circumferential actin microfilament bundles. The expression of CD147, a RPE marker, was detected using a specific antibody in the apical membrane domain (B). The expression of Na+, K+-ATPase using anti-alpha 1(C) & anti-beta 1 antibodies (D) was observed mostly at the basolateral membrane. Immunofluorescence detection with anti-human beta 2 antibody revealed a very weak signal (E). Scale bar: 10 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fphys.2016.00450/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-146_mouse-monoclonal-sodium-potassium-atpase-alpha-1-antibody-464-6-310202416205189.jpg)
![Immunocytochemistry/Immunofluorescence: ATPase Na+/K+ beta 3 Antibody [NBP2-38595] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38595-img0003.jpg "Immunocytochemistry/Immunofluorescence: ATPase Na+/K+ beta 3 Antibody [NBP2-38595] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: ATPase Na+/K+ beta 3 Antibody [NBP2-38595] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-38595-img0004.jpg)
![Western Blot: ATPase Na+/K+ beta 2 Antibody [NBP2-92591] - Analysis of extracts of various cell lines, using ATPase Na+/K+ beta 2 .Exposure time: 30s.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-2-Antibody-Western-Blot-NBP2-92591-img0001.jpg "Western Blot: ATPase Na+/K+ beta 2 Antibody [NBP2-92591] - Analysis of extracts of various cell lines, using ATPase Na+/K+ beta 2 .Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: ATPase Na+/K+ beta 2 Antibody - BSA Free [NBP2-92591] - Immunofluorescence analysis of C6 cells using ATPase Na+/K+ beta 2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92591_rabbit-polyclonal-atpase-na-k-beta-2-antibody-4220251430481.jpg%3Ft=1690983098)
![Western Blot: ATPase Na+/K+ beta 3 Antibody (0B7M7) [NBP3-15829] - Analysis of extracts of various cell lines, using ATPase Na+/K+ beta 3 Rabbit mAb (NBP3-15829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-0B7M7-Western-Blot-NBP3-15829-img0002.jpg "Western Blot: ATPase Na+/K+ beta 3 Antibody (0B7M7) [NBP3-15829] - Analysis of extracts of various cell lines, using ATPase Na+/K+ beta 3 Rabbit mAb (NBP3-15829) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: ATPase Na+/K+ beta 3 Antibody (0B7M7) [NBP3-15829] - Human liver cancer using ATPase Na+/K+ beta 3 Rabbit mAb (NBP3-15829) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-0B7M7-Immunohistochemistry-Paraffin-NBP3-15829-img0001.jpg)
![Western Blot: ATPase Na+/K+ beta 2 Antibody - BSA Free [NBP3-47924] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (ATPase Na+/K+ beta 2 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-47924_rabbit-atpase-na-k-beta-2-pab-bsa-free-28420251141396.jpg "Western Blot: ATPase Na+/K+ beta 2 Antibody - BSA Free [NBP3-47924] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (ATPase Na+/K+ beta 2 Antibody) at dilution of 1:1000")
![Immunohistochemistry: ATPase Na+/K+ beta 2 Antibody - BSA Free [NBP3-47924] - Immunohistochemistry of paraffin-embedded human brain slide using (ATPase Na+/K+ beta 2 Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47924_rabbit-atpase-na-k-beta-2-pab-bsa-free-284202511425572.jpg)
![ELISA: ATPase Na+/K+ beta 3 Antibody (1E9) [H00000483-M03] - Detection limit for recombinant GST tagged ATP1B3 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATPase-Na+-K+-beta-3-Antibody-1E9-ELISA-H00000483-M03-img0001.jpg "ELISA: ATPase Na+/K+ beta 3 Antibody (1E9) [H00000483-M03] - Detection limit for recombinant GST tagged ATP1B3 is approximately 3ng/ml as a capture antibody.")