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hdac1 Products By Gene Name

19 Results for "hdac1"

NB100-56340

HDAC1 Antibody

(2 Reviews)

(15 Publications)

0.1 mg

NB100-56340

Knockout Validated: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-337

Alt Catalog# IMG-337

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-31294

HDAC1 Antibody

100 ul

NBP1-31294

Knockout Validated: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC1 protein stained by HDAC1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. Green: HDAC1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Chromatin Immunoprecipitation: HDAC1 Antibody [NBP1-31294] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC1 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Western Blot: HDAC1 Antibody [NBP1-31294] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC1 protein by 2. 5 ug of HDAC1 antibody 10% SDS-PAGE gel.

<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: HDAC1 Antibody [NBP1-31294] - A. 30 ug Rat2 whole cell lysate/extract 10% SDS-PAGE HDAC1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - Analysis of A431, using HDAC1 antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Human testis, using HDAC1 antibody(10 ug/ml). Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Sample: Paraffin-embedded mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. HDAC1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse colon. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, , , , , , , ,

Applications: , , , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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H00003065-M14

HDAC1 Antibody (5C11)

(1 Publication)

0.1 mg

H00003065-M14

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).

ELISA: HDAC1 Antibody (5C11) [H00003065-M14] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.

Proximity Ligation Assay: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of protein-protein interactions between NFKB1 and HDAC1. HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-52937

HDAC1 Antibody (CL0510)

0.1 ml

NBP2-52937

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human rectum and skeletal muscle tissues using NBP2-52937 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of U-251 cells using the Anti-HDAC1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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H00003065-M06

HDAC1 Antibody (1D6)

0.1 mg

H00003065-M06

Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M06), clone 1D6. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml

Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).

Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).

Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).

Western Blot: HDAC1 Antibody (1D6) [H00003065-M06] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).

ELISA: HDAC1 Antibody (1D6) [H00003065-M06] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-15780

HDAC1 Antibody (2L6O7)

100 ul

NBP3-15780

Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (2L6O7) [NBP3-15780] - Immunofluorescence analysis of NIH/3T3 cells using HDAC1 antibody (NBP3-15780) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: HDAC1 Antibody (2L6O7) [NBP3-15780] - Immunohistochemistry of paraffin-embedded Mouse brain using HDAC1 antibody (NBP3-15780) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (2L6O7) [NBP3-15780] - Immunofluorescence analysis of C6 cells using HDAC1 antibody (NBP3-15780) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: HDAC1 Antibody (2L6O7) [NBP3-15780] - Immunohistochemistry of paraffin-embedded Rat testis using HDAC1 antibody (NBP3-15780) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

<b>Genetic Strategies Validation. </b>Knockout Validated: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-54621

HDAC1 Antibody

50 μg

NBP2-54621

Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (50 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP2-54621] - HeLa cells were stained with the antibody against HDAC1 and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HDAC1 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Chromatin Immunoprecipitation: HDAC1 Antibody [NBP2-54621] - ChIP was performed with the antibody against HDAC1 on sheared chromatin from 4,000,000 HeLa cells. An antibody titration consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative IP control. QPCR was performed with primers specific for the EIF4A2 and GAPDH promoters, used as positive controls, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Western Blot: HDAC1 Antibody [NBP2-54621] - Whole cell extracts (25 ug, lane 1) and nuclear extracts (25 ug, lane 2) from HeLa cells were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left.

ELISA: HDAC1 Antibody [NBP2-54621] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against HDAC1, crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:75,000.

Protein Array: HDAC1 Antibody [NBP2-54621] - The specificity of the antibody against HDAC1 was demonstrated using a human protein microarray containing more than 19,000 human proteins. The antibody was used at a dilution of 1:100,000. The figure shows the Z-score of the signal intensity (mean value of the duplicate spots on the array). The names of the proteins with 5 highest Z-scores are indicated at the bottom. This figure clearly shows the high specificity of the antibody for HDAC1.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-78101

HDAC1 Antibody

(1 Publication)

0.1 mg

NBP1-78101

Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: LNCaP prostate cancer cells. Load: 50 ug per lane.Primary antibody: HDAC1 antibody at 1:1000 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: 55kDa for HDAC1.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Immunofluorescence Microscopy of HDAC1 antibody. Tissue: A431 cells. Fixation: methanol. Antigen retrieval: blocked with normal goat serum.Primary antibody: HDAC1 antibody at 4 ug/mL for 1 h at RT.Secondary antibody: rabbit secondary antibody at 0.2 ug/mL for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as green fluorescent signal. A-tubulin monoclonal antibody detected with ATTO 425 (colored RED). 2-color STED image, Leica Microsystems.

Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human lung tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 10 ug/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 as brown color indicates presence of protein, blue color shows cell nuclei.

Western Blot: HDAC1 Antibody [NBP1-78101] - Western Blot of HDAC1 antibody. Lane 1: 293 whole cell lysate. Load: 35 ug per lane.Primary antibody: HDAC1 antibody at 1:3,500 for overnight at 4C.Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:10,000 for 45 min at RT.Block: 5% BLOTTO overnight at 4C.Predicted/Observed size: ~65 kDa corresponding to human HDAC1. Other band(s): none.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-78101] - Rabbit IgG 611-151-122 (shown in red) for to detect HDAC-1 by immunofluorescence. Anti Keratin monoclonal antibody was used with Dylight 488 goat anti mouse (shown in green) to detect Keratin

Immunohistochemistry: HDAC1 Antibody [NBP1-78101] - Immunohistochemistry of HDAC1 antibody. Tissue: human prostate cancer tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: HDAC1 antibody at 1:500 for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: HDAC1 is nuclear.Staining: HDAC1 precipitated purple with blue counterstain.

Reactivity:

Hu, Mu, Rt, Bv, Pm-Or

Species Glossary

Reactivity: Hu, Mu, Rt, Bv, Pm-Or

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP1-83350

HDAC1 Antibody

(1 Publication)

0.1 ml

NBP1-83350

Western Blot: HDAC1 Antibody [NBP1-83350] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-83350] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining in human colon and skeletal muscle tissues using anti-HDAC1 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining of human colon shows high expression.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-83350] - Staining of human skeletal muscle shows low expression as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-59239

HDAC1 Antibody (C.15200144)

50 μg

NBP2-59239

Western Blot: HDAC1 Antibody (C.15200144) [NBP2-59239] - Whole cell extracts (40 ug) from HeLa cells transfected with HDAC1 siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against HDAC1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (C.15200144) [NBP2-59239] - HeLa cells were stained with the antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa Fluor 594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Chromatin Immunoprecipitation: HDAC1 Antibody (C.15200144) [NBP2-59239] - ChIP assays were performed using human HeLa cells, the antibody against HDAC1 and optimized PCR primer sets for qPCR. ChIP was performed with a ChIP kit on sheared chromatin from 10,000 cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed with primers for the GAPDH promoter and for the coding region of p21, a known target gene of HDAC1. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Western Blot: HDAC1 Antibody (C.15200144) [NBP2-59239] - Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against HDAC1 diluted 1:2,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (kDa) is shown on the left.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-15566

HDAC1 Antibody (2W4I5)

100 ul

NBP3-15566

Western Blot: HDAC1 Antibody (2W4I5) [NBP3-15566] - Western blot analysis of extracts of various cell lines, using HDAC1 Mouse mAb (NBP3-15566) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry-Paraffin: HDAC1 Antibody (2W4I5) [NBP3-15566] - Immunohistochemistry of paraffin-embedded Human esophageal using HDAC1 Mouse mAb (NBP3-15566) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation: HDAC1 Antibody (2W4I5) [NBP3-15566] - Chromatin immunoprecipitation analysis of extracts of K-562 cells, using HDAC1 antibody (NBP3-15566) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-13936

HDAC1 Antibody (PCRP-HDAC1-1B7)

100 μg

NBP3-13936

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using HDAC1 Antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).

Flow Cytometry: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC1 antibody (PCRP-HDAC1-1B7) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Protein Array: HDAC1 Antibody (PCRP-HDAC1-1B7) [NBP3-13936] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC1 Antibody (PCRP-HDAC1-1B7).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-14062AF350
Alexa Fluor 405	NBP3-14062AF405
Alexa Fluor 488	NBP3-14062AF488
Alexa Fluor 532	NBP3-14062AF532
Alexa Fluor 594	NBP3-14062AF594
Alexa Fluor 647	NBP3-14062AF647
Alexa Fluor 700	NBP3-14062AF700
Alexa Fluor 750	NBP3-14062AF750
Allophycocyanin	NBP3-14062APC
Biotin	NBP3-14062B
DyLight 350	NBP3-14062UV
DyLight 405	NBP3-14062V
DyLight 488	NBP3-14062G
DyLight 550	NBP3-14062R
DyLight 594	NBP3-14062DL594
DyLight 650	NBP3-14062C
DyLight 680	NBP3-14062FR
DyLight 755	NBP3-14062IR
FITC	NBP3-14062F
HRP	NBP3-14062H
Janelia Fluor 549	NBP3-14062JF549
Janelia Fluor 646	NBP3-14062JF646
PE	NBP3-14062PE
PerCP	NBP3-14062PCP

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-14062

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