| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB100-56343AFP488 | |||
| Alexa Fluor Plus 555 | NB100-56343AFP555 | |||
| Alexa Fluor Plus 594 | NB100-56343AFP594 | |||
| Alexa Fluor Plus 647 | NB100-56343AFP647 | |||
| Alexa Fluor Plus 680 | NB100-56343AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56343SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78981SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41286-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14206 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26236-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82212-25ul | |||
![Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-56343-img0002.jpg "Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NB100-56343-img0004.jpg)
![Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Simple-Western-NB100-56343-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-78981] - HDAC-6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78981-img0001.jpg "Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-78981] - HDAC-6 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Flow Cytometry: HDAC6 Antibody [NBP1-78981] - Analysis using the Alexa Fluor (R) 488 conjugate of NBP1-78981. Staining of isolated T-cells. Image from verified customer review (Andressa Sodre, Moffitt Cancer Center).](http://images.novusbio.com/fullsize/HDAC6-Antibody-Flow-Cytometry-NBP1-78981-img0003.jpg)
![Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Western-Blot-NBP3-05295-img0004.jpg "Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295] - Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody NBP3-05295, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunocytochemistry-Immunofluorescence-NBP3-05295-img0001.jpg)
![ELISA: HDAC6 Antibody (236) [NBP3-05295] - ELISA analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295. Plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-ELISA-NBP3-05295-img0002.jpg)
![Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295] - Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and NBP3-05295 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunoprecipitation-NBP3-05295-img0003.jpg)
![Flow Cytometry: Mouse Monoclonal HDAC6 Antibody (236) [NBP3-05295] - Separation of K562 cells stained using anti-human HDAC6 (236) purified antibody (concentration in sample 5.33 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/antibody/nbp3-05295_mouse-monoclonal-hdac6-antibody-236-flow-cytometry-1112202412584..jpg)
![Western Blot: HDAC6 Antibody [NBP1-69127] - WB Suggested Anti-Hdac6 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Mouse Liver](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NBP1-69127-img0001.jpg "Western Blot: HDAC6 Antibody [NBP1-69127] - WB Suggested Anti-Hdac6 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Positive Control: Mouse Liver")
![Immunohistochemistry: HDAC6 Antibody [NBP1-69127] - Human Pineal Tissue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-69127-img0004.jpg)
![Immunohistochemistry: HDAC6 Antibody [NBP1-69127] - Human Adult heart Observed Staining: Cytoplasmic Primary Antibody Concentration: 1 : 100 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunohistochemistry-NBP1-69127-img0005.jpg)
![Immunohistochemistry-Paraffin: HDAC6 Antibody [NBP1-69127] - Human testis tissue at an antibody concentration of 4-8ug/ml.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunohistochemistry-Paraffin-NBP1-69127-img0003.jpg)
![Western Blot: HDAC6 Antibody [NBP2-41286] - Analysis of HDAC6 in human testis tissue lysate with HDAC6 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NBP2-41286-img0004.jpg "Western Blot: HDAC6 Antibody [NBP2-41286] - Analysis of HDAC6 in human testis tissue lysate with HDAC6 antibody at 1 ug/ml.")
![Immunohistochemistry: HDAC6 Antibody - BSA Free [NBP2-41286] - Immunohistochemistry of HDAC6 in human testis tissue with HDAC6 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41286_rabbit-polyclonal-hdac6-antibody-2711202419392757.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC6 Antibody - BSA Free [NBP2-41286] - Immunofluorescence of HDAC6 in human testis tissue with HDAC6 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41286_rabbit-polyclonal-hdac6-antibody-2711202419241352.jpg)
![Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082] - Western blot analysis of extracts of various cell lines, using HDAC6 antibody (NBP3-16082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/HDAC6-Antibody-9V1W2-Western-Blot-NBP3-16082-img0002.jpg "Western Blot: HDAC6 Antibody (9V1W2) [NBP3-16082] - Western blot analysis of extracts of various cell lines, using HDAC6 antibody (NBP3-16082) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: HDAC6 Antibody (9V1W2) [NBP3-16082] - Immunohistochemistry of paraffin-embedded human breast cancer using HDAC6 Rabbit mAb (NBP3-16082) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/HDAC6-Antibody-9V1W2-Immunohistochemistry-Paraffin-NBP3-16082-img0001.jpg)
![Immunoprecipitation: HDAC6 Antibody (9V1W2) [NBP3-16082] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug HDAC6 antibody (NBP3-16082). Western blot was performed from the immunoprecipitate using HDAC6 antibody (NBP3-16082) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/HDAC6-Antibody-9V1W2-Immunoprecipitation-NBP3-16082-img0003.jpg)
![Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/HDAC6-Antibody-159-Flow-Cytometry-NBP3-09113-img0001.jpg "Flow Cytometry: HDAC6 Antibody (159) [NBP3-09113] - Separation of K562 cells stained using anti-HDAC6 (159) purified antibody (concentration in sample 4,0 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).")
![Western Blot: HDAC6 Antibody [NBP1-45611] - Analysis of HDAC-6 expression with Jurkat cell lysate.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NBP1-45611-img0003.jpg "Western Blot: HDAC6 Antibody [NBP1-45611] - Analysis of HDAC-6 expression with Jurkat cell lysate.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (PCRP-HDAC6-1A4) [NBP3-13824] - Immunofluorescence analysis of PFA-fixed HeLa cells. HDAC6 Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/HDAC6-Antibody-PCRP-HDAC6-1A4-Immunocytochemistry-Immunofluorescence-NBP3-13824-img0002.jpg "Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (PCRP-HDAC6-1A4) [NBP3-13824] - Immunofluorescence analysis of PFA-fixed HeLa cells. HDAC6 Antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (green).")
![Flow Cytometry: HDAC6 Antibody (PCRP-HDAC6-1A4) [NBP3-13824] - Flow cytometric analysis of PFA-fixed HeLa cells. HDAC6 antibody (PCRP-HDAC6-1A4) followed by goat anti-mouse IgG-CF488 (blue), unstained cells (red).](http://images.novusbio.com/fullsize/HDAC6-Antibody-PCRP-HDAC6-1A4-Flow-Cytometry-NBP3-13824-img0001.jpg)
![Protein Array: HDAC6 Antibody (PCRP-HDAC6-1A4) [NBP3-13824] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HDAC6 Antibody (PCRP-HDAC6-1A4).](http://images.novusbio.com/fullsize/HDAC6-Antibody-PCRP-HDAC6-1A4-Protein-Array-NBP3-13824-img0004.jpg)
![Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-61065-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: HDAC6 Antibody [NB100-61065] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Western-Blot-NB100-61065-img0004.jpg)
![Immunoprecipitation: HDAC6 Antibody [NB100-61065] - Detection of human HDAC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 (lot NB100-61065-2) used for IP at 6 ug per reaction. HDAC6 was also immunoprecipitated by a previous lot of this antibody NB100-61065-1. For blotting immunoprecipitated HDAC6,NB100-61065 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunoprecipitation-NB100-61065-img0009.jpg)
![Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-26220241322159.jpg "Immunoprecipitation: HDAC6 Antibody (2H12) [NBP3-26236] - Immunoprecipitating HDAC6 in HepG2 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26236 in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26236 (3ug) + HepG2 whole cell lysate (500ug). Lane 3: HepG2 whole cell lysate (20ug).")
![Immunohistochemistry: HDAC6 Antibody (2H12) [NBP3-26236] - Image of HDAC6 Antibody (2H12) diluted at 1:112.5 and staining in paraffin-embedded human liver cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-262202414471573.jpg)
![Western Blot: HDAC6 Antibody (2H12) [NBP3-26236] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate. All lanes: HDAC6 Antibody at 1.1ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 132, 115 KDa Observed band size: 160 KDa](http://images.novusbio.com/fullsize/nbp3-26236_rabbit-hdac6-mab-2h12-282202411413619.jpg)
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-82212] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82212-img0003.jpg "Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NBP1-82212] - Staining of human cell line U-251MG shows positivity in nucleus but not nucleoli.")
![Immunohistochemistry-Paraffin: HDAC6 Antibody [NBP1-82212] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.](http://images.novusbio.com/fullsize/HDAC6-Antibody-Immunohistochemistry-Paraffin-NBP1-82212-img0011.jpg)