Albumin: Novus Biologicals

MAB1455

Albumin Antibody (188835) [Unconjugated] - Serum

(3 Reviews)

100 μg

MAB1455

HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Albumin was detected in immersion fixed BG01V human embryonic stem cells differentiated to hepatocytes using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Cells were co-stained using Sheep Anti-Human CEBP alpha (Catalog # AF7094) and NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, Catalog # NL012). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Albumin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455, filled histogram) or isotype control antibody (Catalog # <a class=

MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

Simple Western lane view shows lysates of human serum, loaded at 1:25000. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

VC001) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_lightbox">

<b>Orthogonal Strategies Validation. </b>Serum Albumin mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human liver probed with ACD RNAScope<sup>®</sup>Probe (Catalog # 600941) and stained using ACD RNAscope<sup>®</sup>2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455) at 0.05 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # <a class=

VC001) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_thumb" />

Heterochronic blood exchange effects on muscle regeneration and performance.One day after blood exchange mice were injured by intramuscular injections of CTX into TA. Five days after injury, TA muscles were isolated, cryo-sectioned and analysed. (a) TA muscles from young mice receiving young blood (YY), young mice receiving old blood (YO), old mice receiving young blood (OY) and old mice receiving old blood (OO) were analysed by haematoxylin and eosin (H&E) staining and immunofluorescence with anti-eMyHC antibody. Representative images show an injury site and nascent de-novo formed eMyHC+ myofibers which are smaller in size with central nuclei than uninjured myofibers. Scale bar, 50 μm for H&E panel and 25 μm for immunofluorescence panel. (b,c) Regeneration indices ±s.e.m. were quantified from H&E images (b) and eMyHC images (c) by counting the number of nascent de-novo formed myofibers and dividing by the total number of nuclei present at the injury/regeneration site. By H&E: *P<0.05 N=4 per group. Significant students t test differences exist between YO and OY (P=0.045), YY and OY (P=0.043), YY and OO (P=0.0004), YO and OO (P=0.0042) and between OY and OO (P=0.015). By eMyHC: *P<0.05, N=4 per group; OY and OO P=0.041, YY and OO P=0.00009, and YO to OO P=0.001. (d) Fibrotic/inflammatory indexes were quantified as total injury area minus regenerated myofiber area, per injury site, using the H&E images15. T-test **P<0.005, n=3–4 per group. Muscle from old to old isochronic exchange had diminished regenerative capacity and more fibrosis, as compared with muscle from young to young isochronic exchange. Heterochronic blood exchange significantly improved regeneration of old muscle after experimental injury and reduced fibrosis, but no significant decline in young muscle regeneration was seen. (e) A four-limb hanging test was conducted with isochronically and heterochronically transfused mice that were not injured, before and at 6 days after the blood exchange. Maximal h

Heterochronic blood exchange effects on hepatogenesis and liver fibrosis and adiposity.(a) Livers from YY, YO, OY and OO mice with and without experimental muscle injury as above were cryo-sectioned at 10 μm and immuno-stained for Ki67 (red), hepatocyte marker albumin (green) and Hoechst (blue). Representative images show YY livers with and without injury. Scale bar, 50 μm. (B&C. Quantification of hepatocyte proliferation was by counting the average number of Ki67+,abumin+,Hoechst+ cells per 10 μm section from multiple sections of each blood exchange cohort. (b) Old hepatocyte showed increased proliferation and young hepatocytes showed less proliferation with heterochronic blood as compared with isochronic blood exchanges in animals with injured muscle (t test P=0.00028). (c) This trend continues without muscle injury, but the total numbers of proliferating hepatocytes decline by twofold, (P=0.02411). *P<0.05; **P<0.005; n=3–5. (d) As previously published4, there were fibrotic clusters exclusively in the old livers of small Ki67+ve, albumin negative Ki67+ cells. Scale bar, 50 μm, × 40 magnification. (e,f) Fibrotic index was calculated as the average number of albumin negative proliferative cell clusters per four 10 μm sections. The fibrotic index diminished in old mice exchanged with young blood with muscle injury (e) (t test P=0.048 N=4, *P<0.05) or without (f) (t test P=0.00776. N=3; **P<0.005). (g) Liver adiposity was assayed by Oil Red in 10 μm cryosections. Shown are representative images acquired at × 20 magnification. (h). Liver adiposity (red) was quantified by Image J, dramatically increased with age and was attenuated by young blood in old mice (t test N=3, P=0.022), while adiposity remained unchanged in young mice that were transfused with the old blood (see Supplementary Figure 4). Shown are means±s.e.m. for all histograms. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27874859), licensed under a CC

<b>Biological Strategies Validation. </b>Differentiation of hESCs into HLCs.a The relative hepatocyte (ALB, AAT, CK18, and ASGPR1), cholangiocyte (SOX9), and hepatoblast (AFP) gene expression levels of day 19 differentiated cells with different treatments (groups A and B) were determined by qPCR. HGF (hepatocyte growth factor, 20 ng/mL); OSM (oncostatin M, 20 ng/mL); Dex (dexamethasone, 10 µM); SB431542 (TGF beta inhibitor, 2 µM); and RO4929097 (Notch inhibitor, 1 µM). b Immunofluorescence analysis of ALB, AAT, ASGPR1, and CK18 expression in group B-induced differentiated cells on day 19. c The expression levels of ALB and ASGPR1 in group B-induced differentiated cells were determined by flow cytometry on day 19. Isotype control antibodies were used as controls. d The relative hepatocyte (ALB, AAT, CK18, ASGPR1, and AFP) gene expression levels of differentiated HLCs (group B) compared with those of hESCs and primary human hepatocytes (PHHs) were determined by qPCR. e Albumin secretion of HLCs (black line) on days 12, 14, 16, 18, and 20 and PHHs (dotted line) on day 2 were determined by ELISA. *p < 0.05, **p < 0.01; data are represented as the mean ± SD. Scale bar, 50 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31601782), licensed under a CC-BY license. Not internally tested by R&D Systems.

NLRP3 inflammasome-dependent pyroptosis occurs in liver fibrosis. (A) IHC staining for GSDMD, IL-1 beta , and IL-18 in liver sections from liver fibrosis patients and HCs. Scale bar: 40 µm. (B–D) ELISA analyses of serum levels of GSDMD (B), IL-1 beta (C), and IL-18 (D) in liver fibrosis patients (n = 89) and HCs (n = 60). (E) Representative immunofluorescence images of NLRP3 (red) and albumin (hepatocyte marker) (top), F4/80 (KC marker) (middle) or alpha -SMA (HSC marker) (bottom) (green) from the human fibrotic liver tissues. Scale bar: 40 µm. (F) Schematic diagram of the study. Liver fibrosis was induced by CCl4 injection for 8 weeks. (G) Representative mouse liver histology of H&E, Sirius Red staining, and IHC staining for alpha -SMA, GSDMD, and IL-1 beta . Black scale bar: 100 µm; Red scale bar: 50 µm. (H–J) ELISA analyses for serum levels of GSDMD (H), IL-1 beta (I), and IL-18 (J) in CCl4 group mouse (n = 5) and vehicle group mouse (n = 5). (K) Representative immunofluorescence images of NLRP3 (red) and albumin (hepatocyte marker) (top), F4/80 (KC marker) (middle) or alpha -SMA (HSC marker) (bottom) (green) from the 8-week CCl4-treated mouse liver. The vehicle group mouse liver was used as a control. Scale bar: 40 µm. (L) The qRT-PCR analysis for mRNA levels of IL-1 beta in THP-1 macrophages treated with LPS to induce pyroptosis. (M) ELISA analysis for IL-1 beta expression in supernatants from THP-1. (N) Western blot analysis of COL1A1, alpha -SMA, and TGF-beta expression in LX-2 cells which were exposed to CM from LPS-treated THP-1 macrophages. The protein expression was quantified by densitometry and normalized to beta -actin and are shown as fold changes relative to the control group (right panel). ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36429008), licensed under a CC-BY license. Not internally tested by R&D Systems.

human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue)." class="big_lightbox">

Adult stem cell-derived liver organoids were generated following the steps detailed in the <a class=

human liver organoid culture protocol. Differentiated human liver organoids were stained using a (A) Mouse Anti-Human Serum Albumin Monoclonal Antibody (Catalog # MAB1455; red) and a (B) Goat Anti-Human HNF-3beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2400; red) and counterstained with DAPI (Catalog # 5748; blue)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC1455U-100UG
Alexa Fluor 405	IC1455V-100UG
Alexa Fluor 488	IC1455G
Alexa Fluor 532	FAB1455X
Alexa Fluor 594	IC1455T-100UG
Alexa Fluor 647	IC1455R-100UG
Alexa Fluor 700	IC1455N-100UG
Alexa Fluor 750	IC1455S-100UG
Alexa Fluor Plus 405	FAB1455AFP405-100UG
Alexa Fluor Plus 488	FAB1455AFP488-100UG
Alexa Fluor Plus 555	FAB1455AFP555-100UG
Alexa Fluor Plus 594	FAB1455AFP594-100UG
Alexa Fluor Plus 647	FAB1455AFP647-100UG
Alexa Fluor Plus 680	FAB1455AFP680-100UG
Allophycocyanin	IC1455A-100UG
Biotin	FAB1455B
CoraFluor 1	FAB1455CL1
DyLight 350	FAB1455D
DyLight 405	FAB1455E
DyLight 488	FAB1455K
DyLight 550	FAB1455L
DyLight 594	FAB1455M
DyLight 650	FAB1455W
DyLight 680	FAB1455Y
DyLight 755	FAB1455Z
FITC	FAB1455F
HRP	FAB1455H
Janelia Fluor 525	FAB1455JF525
Janelia Fluor 549	FAB1455I
Janelia Fluor 646	FAB1455J
Janelia Fluor 669	FAB1455JF669
PerCP	FAB1455C
Phycoerythrin	IC1455P
mFluor Violet 450 SE	FAB1455MFV450
mFluor Violet 500 SE	FAB1455MFV500
mFluor Violet 610 SE	FAB1455MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC1455P
Carrier Free	IC1455G
Carrier Free	MAB1455-SP
Carrier Free	IC1455V-100UG
Carrier Free	IC1455U-100UG
Carrier Free	IC1455T-100UG
Carrier Free	IC1455S-100UG
Carrier Free	IC1455R-100UG
Carrier Free	IC1455N-100UG
Carrier Free	IC1455A-100UG

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NB600-41532

Albumin Antibody - BSA Free

(43 Publications)

1 ml

NB600-41532

Western Blot: Albumin Antibody - BSA Free [NB600-41532] - Primary cell characterization.As per the materials & methods, tumors were isolated from mice & cultured. A, Primary murine hepatocytes were used as controls (Mu). Western blot revealed the primary cultures of APN KO A51, A48, A50 & A52 & WT C53, C54 & C55 cells to express p-mTOR, mTOR, p-Src, Src, PCNA, albumin & AFP. B, Immunofluorescence for K8 & pan-cytokeratin (PK) confirmed that A52 cells were epithelial (Scale bar 20 μM). C, Table showing in vitro & in vivo growth characteristics of WT & APN KO primary cultures. Scale of growth (+ to ++++). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30794695), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

NLRP12 downregulates multiple JNK activating pathways in hepatocytes.(A) For checking the purity of primary hepatocytes, hepatocytes isolated from healthy WT and Nlrp12-/- mouse livers were immunostained with antibody for hepatocyte-specific marker Albumin and counterstained with DAPI. (B) Primary hepatocytes from WT and Nlrp12-/- healthy mouse livers were cultured and stimulated with LPS (1 μg/mL), PGN (10 μg/mL), TNF alpha (20 ng/mL), and IL-6 (20 ng/mL) for 4 hr. The expression of indicated genes was analyzed by real-time qPCR. Data represent means +/- SD (n = 3) and representative of three independent experiments. *p<0.05. **p<0.01, ***p<0.001. Statistical difference was determined by two-tailed unpaired t-test. (C) HepG2 cells stably expressing either GFP or NLRP12 were stimulated with LPS (1 μg/mL), PGN (10 μg/mL), TNF alpha (20 ng/mL), and IL-6 (20 ng/mL) for 4 hr, and the expression of indicated genes was analyzed by real-time qPCR. Data represent means +/- SD (n = 3) and representative of three independent experiments. *p<0.05. **p<0.01, ***p<0.001. Statistical difference was determined by two-tailed unpaired t-test. (D) Nlrp12-/- primary hepatocytes were stimulated with LPS in the presences or absence of JNK inhibitor. The levels of cMyc, P-cJun, and P-JNK were measured by Western blotting. (E) NLRP12 was knocked down in HepG2 cells using CRISPR/Cas9 technology. Cells were then stimulated with LPS (1 ug/ml) and the activation of JNK was measured by Western blotting.10.7554/eLife.40396.024Figure 6—figure supplement 1—source data 1.NLRP12 downregulates inflammatory responses in hepatocytes in responses to multiple stimuli.NLRP12 downregulates inflammatory responses in hepatocytes in responses to multiple stimuli. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/30990169), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB600-41532AF350
Alexa Fluor 405	NB600-41532AF405
Alexa Fluor 488	NB600-41532AF488
Alexa Fluor 532	NB600-41532AF532
Alexa Fluor 594	NB600-41532AF594
Alexa Fluor 647	NB600-41532AF647
Alexa Fluor 700	NB600-41532AF700
Alexa Fluor 750	NB600-41532AF750
Allophycocyanin	NB600-41532APC
Biotin	NB600-41532B
DyLight 350	NB600-41532UV
DyLight 405	NB600-41532V
DyLight 488	NB600-41532G
DyLight 550	NB600-41532R
DyLight 594	NB600-41532DL594
DyLight 650	NB600-41532C
DyLight 680	NB600-41532FR
DyLight 755	NB600-41532IR
FITC	NB600-41532F
HRP	NB110-16329-1000ul
Janelia Fluor 525	NB600-41532JF525
Janelia Fluor 549	NB600-41532JF549
Janelia Fluor 646	NB600-41532JF646
Janelia Fluor 669	NB600-41532JF669
PE	NB600-41532PE
PerCP	NB600-41532PCP

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NBP1-32458

Albumin Antibody

(15 Publications)

0.1 ml

NBP1-32458

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Human hepatocellular carcinoma. Albumin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Frozen: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Frozen section of rat liver. Albumin antibody diluted at 1:200.

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Rat liver. Albumin stained by Albumin antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Dog liver.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Dog kidney.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Albumin Antibody [NBP1-32458] - Cat liver.Albumin stained by Albumin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Immunofluorescence localization of TNF-alpha , IL-1 beta & IL-6 in the liver tissue of animals treated with ConA or ConA + hVNS. a–c Representative immunofluorescence images of TNF-alpha , IL-1 beta , & IL-6 that were merged with CD11b. Signal intensities of TNF-alpha , IL-1 beta , & IL-6 from 4 to 6 images were averaged per each animal & compared among experimental groups (n = 3 independent experiments). One-way ANOVA with Tukey post hoc is shown. *p < 0.05, **p < 0.01, ***p < 0.001. d Enlarged views of TNF-alpha , IL-1 beta , & IL-6 signals merged with CD11b & Hoechst nuclear staining images in the liver sections from ConA + hVNS animal. e Immunofluorescence staining of liver tissue with anti-CD11b & anti-albumin antibodies. Representative immunofluorescence images using normal mouse IgG antibody instead of primary antibodies followed by reaction with fluorescein-goat anti-mouse IgG antibody are shown in the last column. Scale bars in a–c, d, & e are 100 μm, 10 μm, & 50 μm respectively Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33272194), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Albumin Antibody [NBP1-32458] - PG-4 whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Albumin Antibody [NBP1-32458] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunocytochemistry/ Immunofluorescence: Albumin Antibody [NBP1-32458] - Albumin antibody detects Albumin protein at cytoplasm by immunofluorescent analysis.</br>Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Albumin stained by Albumin antibody (NBP1-32458) diluted at 1:500.</br>Blue: Hoechst 33342 staining.

Western Blot: Albumin Antibody [NBP1-32458] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Orthogonal Strategies Validation. </b>Western Blot: Albumin Antibody [NBP1-32458] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Human plasma (1 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Albumin antibody (NBP1-32458) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PD-MSCs combined with WKYMVm enhance hepatic regeneration in the BDL rat model. mRNA expression of HNF1 alpha (A), ALB (B), FPR2 (C), and HGFR (D) in BDL rat liver shown by qRT-PCR. (E) Protein levels of ALB, cyclin D1, gp130, IL-6, p/tSTAT3, and GAPDH in BDL rat livers shown by Western blots. (F) Expression of PCNA in BDL rat liver shown by immunohistochemistry. Scale bar = 25 μm. (G) Quantification of the PCNA-positive area in BDL rat livers through immunohistochemistry. (H) Secreted HGF level in BDL rat serum shown by ELISAs. n = 3 rats per group, mean +/- SD, * p < 0.05 by one-way ANOVA. Con, control; NTx, nontransplantation group; Tx, PD-MSC transplantation group; Tx+WK, PD-MSCs with WKYMVm combined transplantation group; wk, week. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35053347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

PD-MSCs combined with WKYMVm can regenerate damaged hepatocytes in vitro. (A) Schematic figure of WB-F344s cell in vitro modeling. (B) Cell viability of the LCA-treated WB-F344s cells shown by CCK-8 assays. Protein expression of ALB (C) and HNF1 alpha (D) shown using Western blots. Protein expression was normalized to GAPDH expression through Western blot bands. (E) Localization and expression of translocated HNF1 alpha in the nucleus shown through immunofluorescence. (F) Quantification of translocated HNF1 alpha -positive WB-F344s versus total WB-F344s using immunofluorescence. n = 3 per group, mean +/- SD, * p < 0.05 by t tests. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35053347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Ca, Fe, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Fe, RM

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-77027

Albumin Antibody - BSA Free

(3 Publications)

0.1 mg

NBP1-77027

Immunohistochemistry: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Mouse Liver.Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-albumin antibody at 10 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti- chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry: Albumin Antibody - BSA Free [NBP1-77027] - Validation of Albumin in Rat Liver.Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-albumin antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-chicken IgY (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Western Blot: Albumin Antibody - BSA Free [NBP1-77027] - Validation in Rat Liver. Loading: 10 ug of lysates per lane.Antibodies: Albumin, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Western Blot: Albumin Antibody - BSA Free [NBP1-77027] - Validation in Human Liver.Loading: 10 ug of lysates per lane.Antibodies: Albumin, , 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Western Blot: Albumin Antibody - BSA Free [NBP1-77027] - Loading: 10 µg of lysates per lane.Antibodies: Albumin, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti- chicken IgY HRP conjugate at 1:10000 dilution.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Chicken Polyclonal

Host: Chicken Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77027-0.025mg

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DY1455

Human Serum Albumin DuoSet ELISA, 15 Plate

(17 Publications)

1 Kit (for 15 Plates)

DY1455

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AF3329

Albumin Antibody [Unconjugated]

(10 Publications)

100 μg

AF3329

HAF017). A specific band was detected for Serum Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human liver tissue and mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for Serum Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human liver tissue and mouse liver tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Serum Albumin at approximately 65-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Serum Albumin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of iBJ6 human induced pluripotent stem cell line undifferentiated (0) and hepatocyte differentiated for 14 and 20 days, loaded at 0.2 mg/mL. A specific band was detected for Albumin at approximately 64 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse Serum Albumin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3329) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF3329U-100UG
Alexa Fluor 405	AF3329V-100UG
Alexa Fluor 488	AF3329G-100UG
Alexa Fluor 532	AF3329X-100UG
Alexa Fluor 594	AF3329T-100UG
Alexa Fluor 647	AF3329R-100UG
Alexa Fluor 700	AF3329N-100UG
Alexa Fluor 750	AF3329S-100UG
Alexa Fluor Plus 405	AF3329AFP405-100UG
Alexa Fluor Plus 488	AF3329AFP488-100UG
Alexa Fluor Plus 555	AF3329AFP555-100UG
Alexa Fluor Plus 594	AF3329AFP594-100UG
Alexa Fluor Plus 647	AF3329AFP647-100UG
Alexa Fluor Plus 680	AF3329AFP680-100UG
Allophycocyanin	AF3329A
Biotin	AF3329B
CoraFluor 1	AF3329CL1
DyLight 350	AF3329D
DyLight 405	AF3329E
DyLight 488	AF3329K
DyLight 550	AF3329L
DyLight 594	AF3329M
DyLight 650	AF3329W
DyLight 680	AF3329Y
DyLight 755	AF3329Z
FITC	AF3329F
HRP	AF3329H
Janelia Fluor 525	AF3329JF525
Janelia Fluor 549	AF3329I
Janelia Fluor 646	AF3329J
Janelia Fluor 669	AF3329JF669
PE	AF3329P
PerCP	AF3329C
mFluor Violet 450 SE	AF3329MFV450
mFluor Violet 500 SE	AF3329MFV500
mFluor Violet 610 SE	AF3329MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF3329-SP

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NB500-417

Albumin Antibody (AL-01) - BSA Free

0.1 mg

NB500-417

Reactivity:

Hu, Bv(-), Mu(-), Po(-), Rb(-)

Species Glossary

Reactivity: Hu, Bv(-), Mu(-), Po(-), Rb(-)

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB500-417AF350
Alexa Fluor 405	NB500-417AF405
Alexa Fluor 488	NB500-417AF488
Alexa Fluor 532	NB500-417AF532
Alexa Fluor 594	NB500-417AF594
Alexa Fluor 647	NB500-417AF647
Alexa Fluor 700	NB500-417AF700
Alexa Fluor 750	NB500-417AF750
Alexa Fluor Plus 405	NB500-417AFP405
Alexa Fluor Plus 488	NB500-417AFP488
Alexa Fluor Plus 555	NB500-417AFP555
Alexa Fluor Plus 594	NB500-417AFP594
Alexa Fluor Plus 647	NB500-417AFP647
Alexa Fluor Plus 680	NB500-417AFP680
Allophycocyanin	NB500-417APC
Biotin	NB500-417B
CoraFluor 1	NB500-417CL1
DyLight 350	NB500-417UV
DyLight 405	NB500-417V
DyLight 488	NB500-417G
DyLight 550	NB500-417R
DyLight 594	NB500-417DL594
DyLight 650	NB500-417C
DyLight 680	NB500-417FR
DyLight 755	NB500-417IR
FITC	NB500-417F
HRP	NB500-417H
Janelia Fluor 525	NB500-417JF525
Janelia Fluor 549	NB500-417JF549
Janelia Fluor 646	NB500-417JF646
Janelia Fluor 669	NB500-417JF669
PE	NB500-417PE
PerCP	NB500-417PCP
mFluor Violet 450 SE	NB500-417MFV450
mFluor Violet 500 SE	NB500-417MFV500
mFluor Violet 610 SE	NB500-417MFV610

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NBP2-66894

Human Serum Albumin Antibody (JF32-10)

100 ul

NBP2-66894

Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded human kidney tissue using anti-Albumin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded human liver tissue using anti-Albumin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded human lung tissue using anti-Albumin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded human spleen tissue using anti-Albumin antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Human Serum Albumin Antibody (JF32-10) [NBP2-66894] - Analysis of paraffin-embedded mouse liver tissue using anti-Albumin antibody. Counter stained with hematoxylin.

Western blot analysis on different lysates with NBP2-66894 at 1/40,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Mouse spleen tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 69 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-66894 at 1/40,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Reactivity:

Hu, Mu, Rt, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Bv

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-97049

Human Serum Albumin Native Protein

5 mg

NBP1-97049

SDS-Page: Albumin Native Protein [NBP1-97049] - SDS-PAGE results of Human Albumin. Lane 1: reduced human albumin. Lane 2: opal prestained molecular weight ladder. Lane 3: non-reduced human albumin. Load: 1ug. 4-20% Lonza SDS-PAGE; Coomassie Stained; ChemiDoc Imaged.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Conjugate	Catalog #	Availability	Size	Price
HRP	NBP1-96988
Rhodamine	NBP1-96881

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NBP3-12180

Bovine Albumin ELISA Kit (Colorimetric)

(1 Publication)

96 wells

NBP3-12180

Reactivity:

Bv

Species Glossary

Reactivity: Bv

Applications:

Applications:

Standard Curve:

0.69 - 500 ng/mL

Sample Type:

Serum, Plasma, Colostrum, Milk, Cell Culture Supernatant

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NBP2-26510

Mouse Serum Albumin Antibody - BSA Free

0.25 mg

NBP2-26510

SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510]

SDS-Page: Mouse Serum Albumin Antibody [NBP2-26510] - SDS-PAGE of Mouse Mouse Serum Albumin antibody.Lane 1: mouse serum albumin reduced. Lane 2: molecular weight. Lane 3: mouse serum albumin non-reduced. Load: 1.0 ug.Predicted/Observed size: Analysis by SDS-PAGE shows a banding pattern consistent with purified Rabbit IgG.Other band(s): none.

ELISA: Mouse Serum Albumin Antibody [NBP2-26510] - ELISA results of purified Mouse Mouse Serum Albumin antibody tested against Mouse Mouse Serum Albumin. Each well was coated in duplicate with 1.0 ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.

Western Blot of rabbit anti-mouse serum albumin. Lane 1: molecular weight. Lane 2: mouse serum albumin. Load: 0.1 ug. Primary antibody: MSA antibody at 1:1000 in 1% BSA-TTBS for overnight at 4C. Secondary antibody: IRDye800(TM) rabbit secondary antibody at 1:20,000 for 45 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP3-12188

Mouse Albumin ELISA Kit (Colorimetric)

96 wells

NBP3-12188

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

1.23 - 900 ng/mL

Sample Type:

Serum, Plama, Urine

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Alb Products By Gene Name

Albumin Antibody (188835) [Unconjugated] - Serum

Albumin Antibody - BSA Free

Albumin Antibody

Albumin Antibody - BSA Free

Human Serum Albumin DuoSet ELISA, 15 Plate

Albumin Antibody [Unconjugated]

Albumin Antibody (AL-01) - BSA Free

Human Serum Albumin Antibody (JF32-10)

Human Serum Albumin Native Protein

Bovine Albumin ELISA Kit (Colorimetric)

Mouse Serum Albumin Antibody - BSA Free

Mouse Albumin ELISA Kit (Colorimetric)