HAF017). A specific band was detected for a
2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">
HAF017). A specific band was detected for a
2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." alt="Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human a
2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog #
HAF017). A specific band was detected for a
2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
1938-PI) was serially diluted and captured by Mouse Anti-Human ?
2‑Macroglobulin Monoclonal Antibody (Catalog #
MAB19381) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human ?
2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">
1938-PI) was serially diluted and captured by Mouse Anti-Human ?
2‑Macroglobulin Monoclonal Antibody (Catalog #
MAB19381) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human ?
2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />
1938-PI) was serially diluted and captured by Mouse Anti-Human alpha
2‑Macroglobulin Monoclonal Antibody (Catalog #
MAB19381) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human alpha
2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">
1938-PI) was serially diluted and captured by Mouse Anti-Human alpha
2‑Macroglobulin Monoclonal Antibody (Catalog #
MAB19381) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Human alpha
2‑Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />
HAF017). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows human serum. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1938) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
HAF016). A specific band for a2-Macroglobulin was detected at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows samples of purified a2-Macroglobulin from mouse sera. PVDF membrane was probed with 0.1 µg/mL Sheep Anti-Mouse a2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5798) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for a2-Macroglobulin was detected at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
1938-PI) was serially diluted and captured by Mouse Anti-Human ?2‑Macroglobulin Monoclonal Antibody (Catalog # 
1938-PI) was serially diluted and captured by Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # ![Western Blot: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Analysis of extracts of HepG2 cells, using Alpha-2-Macroglobulin (alpha 2-Macroglobulin) Rabbit mAb (NBP3-16875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-7V6M10-Western-Blot-NBP3-16875-img0007.jpg "Western Blot: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Analysis of extracts of HepG2 cells, using Alpha-2-Macroglobulin (alpha 2-Macroglobulin) Rabbit mAb (NBP3-16875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Human cervix cancer tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-1110202485424..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Human tonsil tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-1110202485548..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Mouse brain tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-111020248578..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Rat spleen tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-111020248589..jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded human liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-5220251136127.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded mouse liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511365123.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded rat liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511383776.jpg)
![Immunohistochemistry: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Alpha-2-Macroglobulin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511365143.jpg)
![ELISA: Mouse alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP2-60631] - Representative standard curve.](http://images.novusbio.com/fullsize/Mouse-alpha-2-Macroglobulin-ELISA-Kit-Colorimetric-ELISA-NBP2-60631-img0001.jpg "ELISA: Mouse alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP2-60631] - Representative standard curve.")
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha 2-Macroglobulin Antibody-Western Blot-NB600-938-img0010.jpg "Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.")
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Analysis using the Biotin conjugate of NB600-938. Detection of Lane 1: . Lane 2: none. Load: 100 ug per lane. Primary antibody:alpha 2-Macroglobulin biotin conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0006.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Detection of Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0007.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 163 kDa, 163 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0009.jpg)
![N/A alpha 2-Macroglobulin [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_alpha_2Macroglobulin_DY1938_ELISA_1737.jpg "N/A alpha 2-Macroglobulin [Biotin]")
![Western Blot: alpha 2-Macroglobulin Antibody [NBP1-85491] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NBP1-85491-img0006.jpg "Western Blot: alpha 2-Macroglobulin Antibody [NBP1-85491] - Analysis in human cell line HepG2.")
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody [NBP1-85491] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Immunohistochemistry-Paraffin-NBP1-85491-img0002.jpg)
![ELISA: Human alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP3-18793] - Samples were spiked with high concentrations of Human alpha 2-Macroglobulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18793_human-alpha-2-macroglobulin-elisa-kit-colorimetric-132202416254085.jpg "ELISA: Human alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP3-18793] - Samples were spiked with high concentrations of Human alpha 2-Macroglobulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP3-18793] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18793_human-alpha-2-macroglobulin-elisa-kit-colorimetric-432024941586.jpg)
![ELISA: Rat alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP2-78773] - Samples were spiked with high concentrations of Rat alpha 2-Macroglobulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-78773_rat-alpha-2-macroglobulin-elisa-kit-colorimetric-132202416143060.jpg "ELISA: Rat alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP2-78773] - Samples were spiked with high concentrations of Rat alpha 2-Macroglobulin and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat alpha 2-Macroglobulin ELISA Kit (Colorimetric) [NBP2-78773] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-78773_rat-alpha-2-macroglobulin-elisa-kit-colorimetric-432024963960.jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710] - Formalin-fixed, paraffin-embedded human liver stained with alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-A2M-3623-Immunohistochemistry-Paraffin-NBP3-07710-img0002.jpg "Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710] - Formalin-fixed, paraffin-embedded human liver stained with alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).")
![Protein Array: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710] - Analysis of Protein Array containing more than 19,000 full-length human proteins using alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-A2M-3623-Protein-Array-NBP3-07710-img0001.jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (A2M/3623) [NBP3-07710] - Formalin-fixed, paraffin-embedded human liver stained with alpha 2-Macroglobulin Mouse Monoclonal Antibody (A2M/3623).](http://images.novusbio.com/fullsize/nbp3-07710_mouse-monoclonal-alpha-2-macroglobulin-antibody-a2m-3623-1772024104465.jpg)
