| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85358-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5410-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44066 | |||
| BSA Free | NBP2-59781-25ul | |||
HAF019). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5410-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44067 | |||
| BSA Free | NBP2-59782-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98906-50ul | |||
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP1-85358-img0009.jpg)
![Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP1-85358-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody [NBP1-85358] - Staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85358-img0007.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0017.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0010.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0011.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human prostate shows weak granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0015.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human endometrium shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-85358-img0016.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human placenta and skeletal muscle tissues using NBP2-59781 antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human placenta and skeletal muscle tissues using NBP2-59781 antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PPIB monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Western-Blot-NBP2-59781-img0009.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0014.jpg)
![Western Blot: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Western-Blot-NBP2-59781-img0010.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0012.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0013.jpg)
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody (CL3901) [NBP2-59781] - Staining of human skeletal muscle shows negative to very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-CL3901-Immunohistochemistry-Paraffin-NBP2-59781-img0015.jpg)
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for ![Western Blot: Cyclophilin B Antibody [NBP2-24770] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Cyclophilin B Polyclonal Antibody (Catalog # NBP2-24770) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Cyclophilin B at approximately 22 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Knockout-Validated-NBP2-24770-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody [NBP2-24770] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cyclophilin B knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human Cyclophilin B Polyclonal Antibody (Catalog # NBP2-24770) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Cyclophilin B at approximately 22 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody [NBP2-24770] - analysis of Cyclophilin B in A) HeLa cell lysate and B) 293 cell lysate using this antibody. 5 and 2 ug/ml, respectively. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP2-24770-img0001.jpg)
![SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]](http://images.novusbio.com/fullsize/Recombinant-Human-Cyclophilin-B-Protein-SDS-Page-NBC1-18424-img0002.jpg "SDS-Page: Recombinant Human Cyclophilin B Protein [NBC1-18424]")
![Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-PPIB antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Western Blot: Cyclophilin B Antibody (5479) [NBP2-59782] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-5479-Western-Blot-NBP2-59782-img0001.jpg)
![Western Blot: Cyclophilin B Antibody [NBP1-62356] - HepG2 cell lysate, antibody titration 1.25ug/ml. Gel concentration 12%](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP1-62356-img0004.jpg "Western Blot: Cyclophilin B Antibody [NBP1-62356] - HepG2 cell lysate, antibody titration 1.25ug/ml. Gel concentration 12%")
![Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-62356] - Human Liver Tissue, antibody concentration 4-8ug/ml. Cells with positive label: Hepatocytes (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-62356-img0003.jpg)
![Western Blot: Cyclophilin B Antibody [NBP2-98906] - Lane A: Hela whole cell lysateLane B: A431 whole cell lysateLane C: HepG2 whole cell lysateLane D: 293 whole cell lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP2-98906-img0006.jpg "Western Blot: Cyclophilin B Antibody [NBP2-98906] - Lane A: Hela whole cell lysateLane B: A431 whole cell lysateLane C: HepG2 whole cell lysateLane D: 293 whole cell lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:19 kDa(We are unsure as to the identity of these extra bands.)")
![Western Blot: Cyclophilin B Antibody [NBP2-98906] - Anti-Cyclophilin B rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: A431 Whole Cell Lysate. Lane C: HepG2 Whole Cell Lysate. Lane D: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 23 kDa. Observed band size: 19 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Western-Blot-NBP2-98906-img0004.jpg)
![Immunoprecipitation: Cyclophilin B Antibody [NBP2-98906] - Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-PPIB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-PPIB rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 19 kDaObserved band size: 19 kDa](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-Immunoprecipitation-NBP2-98906-img0007.jpg)
![Western Blot: Cyclophilin B Antibody (M019) [NBP3-23271] - Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (NBP3-23271) at 1:500.](http://images.novusbio.com/fullsize/nbp3-23271_mouse-cyclophilin-b-mab-m019-199202318202910.jpg "Western Blot: Cyclophilin B Antibody (M019) [NBP3-23271] - Western blot analysis of human LNCaP (lane 1), MCF7 (lane 2), MDA-MB-231 (lane 3), and MeWo (lane 4) cell lysates, as well as human recombinant full-length cyclophilin B (lane 5) and cyclophilin A (lane 6). The blot was probed with mouse monoclonal anti-cyclophilin B (NBP3-23271) at 1:500.")
![Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody (M019) [NBP3-23271] - Immunocytochemical labeling of cyclophilin B in methanol:acetone (1:1) fixed human A549 lung cancer cells. The cells were labeled with mouse monoclonal anti-cyclophilin B (NBP3-23271). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23271_mouse-cyclophilin-b-mab-m019-18920231735529.jpg)
![ELISA: Rat Cyclophilin B ELISA Kit (Colorimetric) [NBP3-06847] - Samples were spiked with high concentrations of Rat Cyclophilin B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06847_rat-cyclophilin-b-elisa-kit-colorimetric-1322024162619.jpg "ELISA: Rat Cyclophilin B ELISA Kit (Colorimetric) [NBP3-06847] - Samples were spiked with high concentrations of Rat Cyclophilin B and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Cyclophilin B ELISA Kit (Colorimetric) [NBP3-06847] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06847_rat-cyclophilin-b-elisa-kit-colorimetric-432024941525.jpg)
![Western Blot: Cyclophilin B Antibody (7Z4S7) [NBP3-16494] - Western blot analysis of extracts of various cell lines, using Cyclophilin B Rabbit mAb (NBP3-16494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Cyclophilin-B-Antibody-7Z4S7-Western-Blot-NBP3-16494-img0001.jpg "Western Blot: Cyclophilin B Antibody (7Z4S7) [NBP3-16494] - Western blot analysis of extracts of various cell lines, using Cyclophilin B Rabbit mAb (NBP3-16494) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
