| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73034 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73033 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38132-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38716-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26110-50ul | |||
![Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-1556-img0023.jpg "Western Blot: NONO Antibody [NB100-1556] - B-cell whole cell lysate 40ug. Image submitted by a verified customer review")
![Immunocytochemistry: NONO Antibody [NB100-1556] - NONO in human fibroblasts at 1:200 dilution in 1X PBS/1% BSA/0.3% Triton X-100, 16 hours 4C. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunocytochemistry-NB100-1556-img0024.jpg)
![Immunohistochemistry: NONO Antibody [NB100-1556]](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-NB100-1556-img0026.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: NONO Antibody [NB100-1556] - Detection of Human NONO on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lystate (50 microgram) using NB100-1556. NONO was also immunoprecipitated using anti-NONO antibody NB100-1554 or NB100-1555 at 3 microg/mg lysate.](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-1556-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: NONO Antibody [NB100-1556] - Whole cell lysate NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells.](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-1556-img0018.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Sample: FFPE section of human breast carcinoma (left) and mouse squamous cell carcinoma (right). Antibody: Affinity purified rabbit anti- NONO used at a dilution of 1:1,000 (0.2 ug/ml) and 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-1556-img0020.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-1556] - Affinity purified rabbit anti-NONO used at a dilution of 1:1,000 (0.2 ug/ml).](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-1556-img0025.jpg)
![Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Western-Blot-NBP2-02060-img0017.jpg "Western Blot: NONO Antibody (4D9) [NBP2-02060] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: NONO Antibody (4D9) [NBP2-02060] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunocytochemistry-Immunofluorescence-NBP2-02060-img0016.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0015.jpg)
![Flow Cytometry: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NONO antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Flow-Cytometry-NBP2-02060-img0001.jpg)
![Western Blot: NONO Antibody (4D9) [NBP2-02060] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Western-Blot-NBP2-02060-img0002.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0003.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0004.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0006.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0007.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (4D9) [NBP2-02060] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-4D9-Immunohistochemistry-Paraffin-NBP2-02060-img0014.jpg)
![Western Blot: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Western-Blot-NBP2-73034-img0002.jpg "Western Blot: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NONO.")
![Immunocytochemistry/Immunofluorescence: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73034-img0004.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Human Ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0009.jpg)
![Flow Cytometry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-NONO antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73034-img0003.jpg)
![Western Blot: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-NONO monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Western-Blot-NBP2-73034-img0001.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0005.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0006.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0007.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0008.jpg)
![Immunohistochemistry: NONO Antibody (OTI4D9) - Azide and BSA Free [NBP2-73034] - Staining of paraffin-embedded Human prostate tissue using anti-NONO mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI4D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73034-img0010.jpg)
![Western Blot: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Western-Blot-NBP2-46194-img0010.jpg "Western Blot: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NONO.")
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0009.jpg)
![Western Blot: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Western-Blot-NBP2-46194-img0011.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0002.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0003.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0004.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0005.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0006.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Adenocarcinoma of Human ovary tissue using NONO mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0007.jpg)
![Immunohistochemistry: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunohistochemistry-NBP2-46194-img0008.jpg)
![Immunofluorescence: NONO Antibody (OTI3F8) [NBP2-46194] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY NONO.](http://images.novusbio.com/fullsize/NONO-Antibody-OTI3F8-Immunofluorescence-NBP2-46194-img0001.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241214445.jpg "Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.")
![Immunocytochemistry/ Immunofluorescence: NONO Antibody [NBP3-38132] - Immunofluorescence analysis of NIH-3T3 cells using NONO Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-1012202411545526.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Rat lung using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241233821.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241233879.jpg)
![Western Blot: NONO Antibody [NBP3-38132] - Western blot analysis of various lysates using NONO Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241252848.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-10122024120670.jpg)
![Immunocytochemistry/ Immunofluorescence: NONO Antibody [NBP3-38132] - Immunofluorescence analysis of C6 cells using NONO Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-1012202411572617.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Rat liver using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-10122024122415.jpg)
![Immunohistochemistry: NONO Antibody [NBP3-38132] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using NONO Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-1012202411535826.jpg)
![Immunocytochemistry/ Immunofluorescence: NONO Antibody [NBP3-38132] - Immunofluorescence analysis of U-2 OS cells using NONO Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38132_rabbit-nono-pab-101220241214434.jpg)
![Western Blot: NONO Antibody (0V0N8) [NBP3-16276] - Western blot analysis of extracts of various cell lines, using NONO Rabbit mAb (NBP3-16276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Western-Blot-NBP3-16276-img0006.jpg "Western Blot: NONO Antibody (0V0N8) [NBP3-16276] - Western blot analysis of extracts of various cell lines, using NONO Rabbit mAb (NBP3-16276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: NONO Antibody (0V0N8) [NBP3-16276] - Immunofluorescence analysis of U-2 OS cells using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Immunocytochemistry-Immunofluorescence-NBP3-16276-img0005.jpg)
![Western Blot: NONO Antibody (0V0N8) [NBP3-16276] - Western blot analysis of extracts of Rat liver, using NONO Rabbit mAb (NBP3-16276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Western-Blot-NBP3-16276-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: NONO Antibody (0V0N8) [NBP3-16276] - Immunofluorescence analysis of C6 cells using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Immunocytochemistry-Immunofluorescence-NBP3-16276-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: NONO Antibody (0V0N8) [NBP3-16276] - Immunofluorescence analysis of NIH-3T3 cells using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Immunocytochemistry-Immunofluorescence-NBP3-16276-img0004.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry of paraffin-embedded human esophageal cancer using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/NONO-Antibody-0V0N8-Immunohistochemistry-Paraffin-NBP3-16276-img0001.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-5220251242568.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202511392237.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202511392225.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202511365167.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-52202511402788.jpg)
![Immunohistochemistry: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using NONO Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16276_rabbit-nono-mab-0v0n8-5220251215465.jpg)
![Western Blot: NONO Antibody [NBP2-38716] - Analysis using Anti-NONO antibody NBP2-38716 (A) shows similar pattern to independent antibody NBP2-38727 (B).](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NBP2-38716-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NONO Antibody [NBP2-38716] - Analysis using Anti-NONO antibody NBP2-38716 (A) shows similar pattern to independent antibody NBP2-38727 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: NONO Antibody [NBP2-38716] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38716-img0004.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human kidney.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0009.jpg)
![Immunohistochemistry: NONO Antibody [NBP2-38716] - Staining of human heart muscle shows strong nuclear positivity in myocytes.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-NBP2-38716-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human colon, kidney, liver and testis using Anti-NONO antibody NBP2-38716 (A) shows similar protein distribution across tissues to independent antibody NBP2-38727 (B).](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0005.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human colon.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0007.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human liver.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0008.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38716] - Staining of human testis.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38716-img0010.jpg)
![Western Blot: NONO Antibody [NBP2-38727] - Analysis in human cell line NTERA-2.](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NBP2-38727-img0012.jpg "Western Blot: NONO Antibody [NBP2-38727] - Analysis in human cell line NTERA-2.")
![Immunocytochemistry/Immunofluorescence: NONO Antibody [NBP2-38727] - Staining of human cell line HeLa shows positivity in nucleus but excluded from the nucleoli.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38727-img0001.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human liver.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0010.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human colon, kidney, liver and testis using Anti-NONO antibody NBP2-38727 (A) shows similar protein distribution across tissues to independent antibody NBP2-38716 (B).](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0006.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human colon.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0008.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human testis.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0009.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NBP2-38727] - Staining of human kidney.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NBP2-38727-img0011.jpg)
![Western Blot: NONO Antibody [NB100-40789] - Analysis of NONO using this antibody at 0.1 ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).](http://images.novusbio.com/fullsize/NONO-Antibody-Western-Blot-NB100-40789-img0005.jpg "Western Blot: NONO Antibody [NB100-40789] - Analysis of NONO using this antibody at 0.1 ug/ml in Mouse Brain lysate (35ug protein in RIPA buffer).")
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-40789-img0006.jpg)
![Immunohistochemistry-Paraffin: NONO Antibody [NB100-40789] - (3.8ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/NONO-Antibody-Immunohistochemistry-Paraffin-NB100-40789-img0007.jpg)
![Immunohistochemistry: NONO Antibody (8C11) [NBP3-26110] - Image of NONO Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26110_rabbit-nono-mab-8c11-26220241535575.jpg "Immunohistochemistry: NONO Antibody (8C11) [NBP3-26110] - Image of NONO Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: NONO Antibody (8C11) [NBP3-26110] - Image of NONO Antibody (8C11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26110_rabbit-nono-mab-8c11-27220248573317.jpg)
![Western Blot: NONO Antibody (8C11) [NBP3-26110] - Positive Western Blot detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Ntera-2 whole cell lysate. All lanes: NONO Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55, 44 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26110_rabbit-nono-mab-8c11-28220241135164.jpg)
![Immunocytochemistry/Immunofluorescence: NONO Antibody (8C11) [NBP3-26110] - Staining of Hela Cells with NONO Antibody (8C11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26110_rabbit-nono-mab-8c11-28220241312227.jpg)
![Western Blot: NONO Overexpression Lysate (Adult Normal) [NBP2-09747] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NONO.](http://images.novusbio.com/fullsize/NONO-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09747-img0001.jpg "Western Blot: NONO Overexpression Lysate (Adult Normal) [NBP2-09747] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NONO.")