| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF749-SP | |||
629-LR) is immobilized at 25 ng/mL, 100 µg/well, Biotinylated Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) binds with an ED50 of 0.75-4.5 ng/mL." alt="When Recombinant Human Lymphotoxin beta R/TNFRSF3 Fc Chimera (Catalog # 629-LR) is immobilized at 25 ng/mL, 100 µg/well, Biotinylated Recombinant Human Lymphotoxin alpha1/beta2 His-tag Avi-tag (Catalog # AVI8884) binds with an ED50 of 0.75-4.5 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81105-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21751-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79553-5Plates | |||
![ELISA: Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP3-18772] - Samples were spiked with high concentrations of Mouse Lymphotoxin-alpha/TNF-beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-18772_mouse-lymphotoxin-alpha-tnf-beta-elisa-kit-colorimetric-132202416254018.jpg "ELISA: Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP3-18772] - Samples were spiked with high concentrations of Mouse Lymphotoxin-alpha/TNF-beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Lymphotoxin-alpha/TNF-beta ELISA Kit (Colorimetric) [NBP3-18772] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-18772_mouse-lymphotoxin-alpha-tnf-beta-elisa-kit-colorimetric-4320249192834.jpg)
![Western Blot: Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) - Chimeric [NBP2-81105] - Western Blot using Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [NBP2-81105]. Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 3F12.2D3 [NBP2-81105] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated. NBP2-81105 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.](http://images.novusbio.com/fullsize/Lymphotoxin-alpha-TNF-beta-Antibody-3F12-2D3-Chimeric-Western-Blot-NBP2-81105-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) - Chimeric [NBP2-81105] - Western Blot using Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [NBP2-81105]. Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 3F12.2D3 [NBP2-81105] at 2 ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kDa, though the form shown here is likely glycosylated. NBP2-81105 successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) - Chimeric [NBP2-81105] - Flow-cytometry using the Lymphotoxin-alpha/TNF-beta Antibody (3F12.2D3) [NBP2-81105]. U937 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (isotype control, black line) or the rabbit IgG-chimeric version of 3F12.2D3 (NBP2-81105, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Lymphotoxin-alpha-TNF-beta-Antibody-3F12-2D3-Chimeric-Flow-Cytometry-NBP2-81105-img0001.jpg)
![SDS-Page: Recombinant Human Lymphotoxin-alpha/TNF-beta Protein [NBP2-61307]](http://images.novusbio.com/fullsize/Recombinant-Human-Lymphotoxin-alpha-TNF-beta-Protein-SDS-Page-NBP2-61307-img0002.jpg "SDS-Page: Recombinant Human Lymphotoxin-alpha/TNF-beta Protein [NBP2-61307]")
![Western Blot: Lymphotoxin-alpha/TNF-beta Antibody (SR1300) [NBP3-21751] - Western blot analysis using NBP3-21751 in Lymphotoxin-alpha/TNF-beta recombinant protein.](http://images.novusbio.com/fullsize/nbp3-21751_rabbit-lymphotoxin-alpha-tnf-beta-mab-sr1300-57202316494219.jpg "Western Blot: Lymphotoxin-alpha/TNF-beta Antibody (SR1300) [NBP3-21751] - Western blot analysis using NBP3-21751 in Lymphotoxin-alpha/TNF-beta recombinant protein.")
![Sandwich ELISA: Lymphotoxin-alpha/TNF-beta Antibody Pair [HRP] [NBP2-79553] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/Lymphotoxin-alpha-TNF-beta-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79553-img0001.jpg "Sandwich ELISA: Lymphotoxin-alpha/TNF-beta Antibody Pair [HRP] [NBP2-79553] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
