| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90322-25ul | |||
CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Purkinje neurons." alt="5-HT2B was detected in immersion fixed paraffin-embedded sections of mouse cerebellum using Mouse Anti-Human 5-HT2B Monoclonal Antibody (Catalog # MAB10322) at 8 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to Purkinje neurons." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB10322-SP | |||
MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. " alt="HEK293 cells transfected with Human HTR2B and eGFP vs Irrelevant were stained with eGFP and either (A) Rabbit Anti-Human 5-HT2B Monoclonal Antibody (Catalog # MAB11297) or (B) Normal Rabbit IgG Control (Catalog # MAB1050) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 532 | FAB11297X-100UG | |||
| Allophycocyanin | FAB11297A-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11297-SP | |||
| Carrier Free | FAB11297A-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26870-25ul | |||
| Azide and BSA Free | NBP3-44144 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98911-50ul | |||
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human endometrium shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0010.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human kidney shows moderate membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0008.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NBP1-90322] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NBP1-90322-img0009.jpg)
![Western Blot: 5-HT2B Antibody [NBP1-55429] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Western-Blot-NBP1-55429-img0004.jpg "Western Blot: 5-HT2B Antibody [NBP1-55429] - Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.")
![Immunocytochemistry/Immunofluorescence: 5-HT2B Antibody [NBP1-55429] - Spinal Cord, ventral horns of mouse, 1.3ug/mL Image Submitted By: Timur Mavlyutov, PhD Department of Pharmacology University of Wisconsin Medical School](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-55429-img0002.jpg)
![Western Blot: 5-HT2B Antibody [NBP1-55429] - Titration: 0.2-1 ug/ml, Positive Control: 721_B cell lysate.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Western-Blot-NBP1-55429-img0001.jpg)
![Western Blot: 5-HT2B Antibody [NBP1-55429] - Human Adult Placenta, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Western-Blot-NBP1-55429-img0003.jpg)
![Western Blot: 5-HT2B Antibody [NBP1-55429] - Human Fetal Muscle, Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Western-Blot-NBP1-55429-img0005.jpg)
NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for ![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1111-img0006.jpg "Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Analysis of anti-5HT2B Receptor antibody with human brain, cortex.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1111-img0004.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1111] - Human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1111-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Brain, Amygdala neurons](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0008.jpg "Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Brain, Amygdala neurons")
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Analysis of anti-5HT2B Receptor antibody with human brain, neurons and glia.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0003.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Anti-HTR1B / 5-HT2B Receptor antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0006.jpg)
![Immunohistochemistry-Paraffin: 5-HT2B Antibody [NLS1187] - Brain, Substantia Nigra](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Immunohistochemistry-Paraffin-NLS1187-img0007.jpg)
![SDS-Page: Recombinant Human 5-HT2B Protein [H00003357-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00003357-P01-1.jpg "SDS-Page: Recombinant Human 5-HT2B Protein [H00003357-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: 5-HT2B Antibody (4A4) [H00003357-M01-100ug] - Detection against Immunogen (31.9 KDa) .](http://images.novusbio.com/fullsize/5-HT2B-Antibody-4A4-Western-Blot-H00003357-M01-100ug-img0002.jpg "Western Blot: 5-HT2B Antibody (4A4) [H00003357-M01-100ug] - Detection against Immunogen (31.9 KDa) .")
![ELISA: 5-HT2B Antibody (4A4) [H00003357-M01-100ug] - Detection limit for recombinant GST tagged HTR2B is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/5-HT2B-Antibody-4A4-ELISA-H00003357-M01-100ug-img0001.jpg)
![Western Blot: 5-HT2B Antibody [NBP2-98911] - Anti-5-HT2B rabbit polyclonal antibody at 1:500 dilution. Lane A: HT-29 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: Mouse spleen tissue Lysate. Lane D: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 57 kDa](http://images.novusbio.com/fullsize/5-HT2B-Antibody-Western-Blot-NBP2-98911-img0001.jpg "Western Blot: 5-HT2B Antibody [NBP2-98911] - Anti-5-HT2B rabbit polyclonal antibody at 1:500 dilution. Lane A: HT-29 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: Mouse spleen tissue Lysate. Lane D: 293 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 57 kDa")
