452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (
AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND
50 is typically 0.015‑0.075 µg/mL." class="big_lightbox">

452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (
AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND
50 is typically 0.015‑0.075 µg/mL." alt="Recombinant Mouse CXCL2/MIP-2 (
452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (
AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL2/MIP-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA). The ND
50 is typically 0.015‑0.075 µg/mL." class="big_thumb" />
VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog #
VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog #
VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
452-M2) was serially diluted and captured by Rat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Monoclonal Antibody (Catalog #
MAB452) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">

452-M2) was serially diluted and captured by Rat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Monoclonal Antibody (Catalog #
MAB452) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />