599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP-2 (Catalog #
355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP-2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND
50 is typically 3-15 µg/mL." class="big_lightbox">
599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP-2 (Catalog #
355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP-2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND
50 is typically 3-15 µg/mL." alt="Recombinant Rat Jagged 1 Fc Chimera (Catalog #
599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP-2 (Catalog #
355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP-2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND
50 is typically 3-15 µg/mL." class="big_thumb" />
599-JG) was serially diluted and captured by Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">
599-JG) was serially diluted and captured by Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />
599-JG) was serially diluted and captured by Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF599) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">
599-JG) was serially diluted and captured by Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF599) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />
599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP-2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND50 is typically 3-15 µg/mL." alt="Recombinant Rat Jagged 1 Fc Chimera (Catalog # 599-JG) induces differentiation in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line), as measured by alkaline phosphatase activity. Differentiation elicited by Recombinant Rat Jagged 1 Fc Chimera (3 µg/mL) in the presence of Recombinant Human BMP-2 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599). The ND50 is typically 3-15 µg/mL." class="big_thumb" />
HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Huh-7 human hepatoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1277) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for Jagged 1 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB12771). The ND50 is typically 0.5-3 µg/mL." alt="Recombinant Human Jagged 1 Fc Chimera (Catalog # 1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human Jagged 1 Fc Chimera (5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Jagged 1 Monoclonal Antibody (Catalog # MAB12771). The ND50 is typically 0.5-3 µg/mL." class="big_thumb" />
355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.00-6.00 ug/mL. " alt="Immobilized Recombinant Mouse Jagged 1 Fc Chimera (Catalog # 10969-JG) enhances BMP-2 (355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.00-6.00 ug/mL. " class="big_thumb" />
355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.3-1.8 µg/mL." alt="Immobilized Recombinant Human Jagged 1 N-Terminal Fc Chimera (Catalog # 10111-JG) enhances Recombinant Human/Mouse/Rat BMP-2 (Catalog # 355-BM) induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.3-1.8 µg/mL." class="big_thumb" />
355-BM) alkaline phosphatase inducing activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2.4 µg/mL." alt="Immobilized Recombinant Rat Jagged 1 (Catalog # 9907-JG) enhances Recombinant Human/Mouse/Rat BMP-2 Recombinant Human BMP-2 (Catalog # 355-BM) alkaline phosphatase inducing activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.4-2.4 µg/mL." class="big_thumb" />
599-JG) was serially diluted and captured by Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF599) coated on a Clear Polystyrene Microplate (Catalog # 
599-JG) was serially diluted and captured by Goat Anti-Rat Jagged 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # 
CTS008) and counterstained with hematoxylin (blue). View our protocol for 
1277-JG) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line in the presence of Recombinant Human/Mouse/Rat BMP-2 (![Western Blot: Jagged 1 Antibody (1E12.) [H00000182-M01A] - Western Blot detection against Immunogen (35.64 KDa) .](http://images.novusbio.com/fullsize/Jagged-1-Antibody-1E12-Western-Blot-H00000182-M01A-img0001.jpg "Western Blot: Jagged 1 Antibody (1E12.) [H00000182-M01A] - Western Blot detection against Immunogen (35.64 KDa) .")
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Targeted loss of Jagged1 in adult mouse neurons causes a spatial memory deficit. A schematic representation of the Jagged1 floxed allele used to generate the mice with TAM-inducible loss of Jagged1 gene (A). Representative immunofluorescence images of single confocal z-plane showing a near complete loss of Jagged1 protein (green) from hippocampal pyramidal neurons labeled by NeuN (red) of Jagged1cKO mice (B). Representative images from immunoblots showing the expression of Jagged1, DNER in Jagged1cKO mice & control mice (C). GAPDH is used as a loading control. Bar graph showing the quantitation of optical densities of Jagged1 & DNER bands in CTLs & Jagged1cKO mice (D). A graphic showing the behavioral arena for Y-maze spontaneous alternation test (E). Jagged1cKO mice show a significant deficit in hippocampus dependent working memory in the Y-maze spontaneous alternation test (F). A diagram showing the experimental arena for the hidden arm version of the Y-maze (G). Jagged1cKO mice show a significant reduction in the time spent in the hidden arm, suggesting a spatial memory defect (H). A schematic showing the experimental setup for the Novel Object Displacement test (I). Jagged1cKO mice exhibit a statistical significant reduction in discrimination index, a measure of spatial memory defect (J). Scale bar in (B) is 50 μm. Graphs are represented as mean ± SEM, *p < 0.05, **p < 0.01, & ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415485115.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged1 regulates learning-dependent Notch induction & is enriched presynaptically. Fluorescent double-labeling shows the expression of Notch1 (red) following spatial exploration in CTLs & Jagged1cKO (A). Jagged1 labeling (green) is used to validate the absence of Jagged1 in the Jagged1cKO (Jag1cKOs) (A,C). Diagram summarizing the Notch1 fluorescence intensities distribution in randomly picked 69 & 71 CA1 neurons in CTLs & Jagged1cKOs, respectively (p < 0.001) (B). Double immunofluorescence of the Notch1 transcriptional target, Hes5, in CTLs & JaggedcKOs following spatial exploration (C). Diagram summarizing the Hes5 fluorescence intensity distribution in randomly picked 64 & 70 CA1 neurons in CTL & Jagged1cKO, respectively (p < 0.001) (D). Representatives gold Immuno-electron microscopy panels from three WT mice show that Jagged1 particles are localized in presynaptic terminals, bound to presynaptic vesicles (Pre) (E–E‴). Immunogold particles are also apparent at the Postsynaptic density (Post) (E,E″,E‴). Box Plot summarizing the particle counts in presynaptic & postsynaptic terminal areas drawn on Immunoelectromicroscopy micrograph for Jagged1 indicates that the presynapse is enriched with Jag1 particles as compared to the postsynaptic terminal (p < 0.001) (F). Scale bars are: 20 μm in (A), 10 μm in (C), 400 nm in (E–E‴). AU, arbitrary units; Pre, presynaptic; Post, postsynaptic; & Ax, axon. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415483644.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged1 expression in brains & CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) & Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls & AD patients (A–C). in healthy controls, Jagged1 is localized to somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) & (C,C′) core plaques double positive for Notch1 & Thioflavin-T. Jag1 expression is scattered in parenchyma & low in, degenerated neurons (white arrows) (B,B′). Jag1 overlays in small double positive aggregates for Notch1 & Thioflavin-T (100x magnification) in radiating plaques with a visible reduction in Jagged1 cellular expression (B–C′). Box plots summarizing the quantification of fluorescence intensities of Jagged1 immunolabeled neurons shows a significant reduction in Jagged1 expression in AD patients (p < 0.001). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415485139.jpg)
![Immunocytochemistry/ Immunofluorescence: Jagged 1 Antibody (1E12) [H00000182-M01A] - Jagged1 expression in brains & CSF of AD patients. Representative double fluorescent immunolabelings for Jagged1 (green) & Notch1 (red) counterstained with Thioflavin-T or DAPI (blue) on postmortem brain sections comprising the hippocampal CA fields from healthy age-matched controls & AD patients (A–C). in healthy controls, Jagged1 is localized to somata of neurons where also Notch1 is expressed. As expected, Thioflavin-T labeling is negligible (A,A′). AD sections show fibrillary aggregates (B,B′) & (C,C′) core plaques double positive for Notch1 & Thioflavin-T. Jag1 expression is scattered in parenchyma & low in, degenerated neurons (white arrows) (B,B′). Jag1 overlays in small double positive aggregates for Notch1 & Thioflavin-T (100x magnification) in radiating plaques with a visible reduction in Jagged1 cellular expression (B–C′). Box plots summarizing the quantification of fluorescence intensities of Jagged1 immunolabeled neurons shows a significant reduction in Jagged1 expression in AD patients (p < 0.001). Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fncel.2017.00220/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00000182-m01a_mouse-monoclonal-jagged-1-antibody-1e12-310202415482294.jpg)
![Western Blot: Jagged 1 Antibody [NB600-1161] - Analysis of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at 134 kDa in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kDa.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Western-Blot-NB600-1161-img0004.jpg "Western Blot: Jagged 1 Antibody [NB600-1161] - Analysis of Jagged-1 protein in various whole cell lysates: human brain (lane 1), human kidney (lane 2), human liver (lane 3), and mouse liver (lane 5). Lane 4 contained sample buffer only. The band at 134 kDa in lane 5 is believed to be Jagged-1 precursor. The identity of minor reactive bands is unknown. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG. Exposure time was 1 min. Predicted molecular weight is 134 kDa.")
![Immunocytochemistry/Immunofluorescence: Jagged 1 Antibody [NB600-1161]](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1161-img0005.jpg)
![Immunohistochemistry: Jagged 1 Antibody [NB600-1161] - Human cervical cancer tissue (40X magnification) using NB600-1161 Protein A purified anti- Jagged-1 antibody. Tissue was fixed with formalin and embedded in paraffin. Hematoxylin was used to counter-stain cells. A 1:100 dilution of primary antibody was used.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-NB600-1161-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Jagged 1 Antibody [NB600-1161] - Human corneal epithelial cells. Primary antibody was used at a 1:500 dilution. The Jagged1 (green staining) is localized to the cytoplasm and is consistent with reports in the literature. The nucleus is stained with Bis benzimide (blue).](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1161-img0002.jpg)
![Western Blot: Jagged 1 Antibody (OTI3A10) [NBP2-45467] - Jagged 1 Antibody (3A10) [NBP2-45467] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Jagged 1.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-OTI3A10-Western-Blot-NBP2-45467-img0009.jpg "Western Blot: Jagged 1 Antibody (OTI3A10) [NBP2-45467] - Jagged 1 Antibody (3A10) [NBP2-45467] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Jagged 1.")
![Immunohistochemistry: Jagged 1 Antibody (3A10) [NBP2-45467] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Jagged-1-Antibody-3A10-Immunohistochemistry-NBP2-45467-img0007.jpg)
![Immunohistochemistry: Jagged 1 Antibody (3A10) [NBP2-45467] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Jagged-1-Antibody-3A10-Immunohistochemistry-NBP2-45467-img0001.jpg)
![Immunohistochemistry: Jagged 1 Antibody (3A10) [NBP2-45467] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Jagged-1-Antibody-3A10-Immunohistochemistry-NBP2-45467-img0002.jpg)
![Immunohistochemistry: Jagged 1 Antibody (3A10) [NBP2-45467] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Jagged-1-Antibody-3A10-Immunohistochemistry-NBP2-45467-img0003.jpg)
![Immunohistochemistry: Jagged 1 Antibody (3A10) [NBP2-45467] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Jagged-1-Antibody-3A10-Immunohistochemistry-NBP2-45467-img0004.jpg)
![Immunohistochemistry: Jagged 1 Antibody (3A10) [NBP2-45467] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Jagged-1-Antibody-3A10-Immunohistochemistry-NBP2-45467-img0005.jpg)
![Immunohistochemistry: Jagged 1 Antibody (3A10) [NBP2-45467] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Jagged-1-Antibody-3A10-Immunohistochemistry-NBP2-45467-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0012.jpg)
![Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human lymph node shows no cytoplasmic positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0011.jpg)
![Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human duodenum shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0010.jpg)
![Immunohistochemistry-Paraffin: Jagged 1 Antibody [NBP1-90208] - Staining of human skin shows weak cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Jagged-1-Antibody-Immunohistochemistry-Paraffin-NBP1-90208-img0013.jpg)
