HAF016). Specific bands were detected for Dynactin Subunit 1/DCTN1 at approximately 150 and 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of human brain tissue, human testis tissue, mouse brain tissue, and rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Human Dynactin Subunit 1/DCTN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6657) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Dynactin Subunit 1/DCTN1 at approximately 150 and 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6657-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6657-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21351-25ul | |||
![Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183342.jpg "Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).")
![Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (1ug/ml) staining of HeLa (A) and (0.5ug/ml) MCF7 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-1652023918334.jpg)
![Flow Cytometry: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183312.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183347.jpg)
![Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183314.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NB100-1110] - (7ug/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-1110_goat-polyclonal-dynactin-subunit-1-dctn1-antibody-16520239183344.jpg)
![Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Analysis of methanol-fixed HeLa, using DCTN1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30880-img0007.jpg "Immunocytochemistry/Immunofluorescence: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Analysis of methanol-fixed HeLa, using DCTN1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Paraffin-embedded Rat brain. DCTN1 antibody [C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Immunohistochemistry-Paraffin-NBP1-30880-img0011.jpg)
![Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Western-Blot-NBP1-30880-img0013.jpg)
![Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Western-Blot-NBP1-30880-img0015.jpg)
![Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DCTN1 antibody [C3], C-term diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Western-Blot-NBP1-30880-img0017.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP1-30880] - Paraffin-embedded OVCA, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Immunohistochemistry-Paraffin-NBP1-30880-img0008.jpg)
![Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Dynactin Subunit 1/DCTN1 antibody [C3], C-term (NBP1-30880) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-30880_rabbit-polyclonal-dynactin-subunit-1-dctn1-antibody-western-blot-141120251842522.jpg)
![Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP2-33976] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Western-Blot-NBP2-33976-img0004.jpg "Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP2-33976] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP2-33976] - Staining of human Fallopian tube shows strong membranous and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33976-img0008.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP2-33976] - Staining of human stomach, lower shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33976-img0001.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP2-33976] - Staining of human Cerebellum shows weak cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33976-img0006.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP2-33976] - Staining of human Cerebral cortex shows weak cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33976-img0007.jpg)
![Immunohistochemistry-Paraffin: Dynactin Subunit 1/DCTN1 Antibody [NBP2-33976] - Staining of human Testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and leydig cells.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Immunohistochemistry-Paraffin-NBP2-33976-img0009.jpg)
CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # 
![Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-71829] - Whole cell lysate from Jurkat (5, 15 and 50 mcg for WB), HeLa (H, 50 mcg; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-p150glued antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. p150glued was also immunoprecipitated by rabbit anti-p150glued antibody NBP1-71828, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-Western-Blot-NBP1-71829-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Dynactin Subunit 1/DCTN1 Antibody [NBP1-71829] - Whole cell lysate from Jurkat (5, 15 and 50 mcg for WB), HeLa (H, 50 mcg; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-p150glued antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. p150glued was also immunoprecipitated by rabbit anti-p150glued antibody NBP1-71828, which recognizes an upstream epitope.")
![Recombinant Human Dynactin Subunit 1/DCTN1 Protein [H00001639-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00001639-P01-1.jpg "Recombinant Human Dynactin Subunit 1/DCTN1 Protein [H00001639-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: DCTN1 Overexpression Lysate (Adult Normal) [NBL1-09749] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DCTN1.](http://images.novusbio.com/fullsize/DCTN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09749-img0002.jpg "Western Blot: DCTN1 Overexpression Lysate (Adult Normal) [NBL1-09749] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DCTN1.")
![Sandwich ELISA: Dynactin Subunit 1/DCTN1 Antibody (1E12) [H00001639-M01] - Detection limit for recombinant GST tagged DCTN1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-1E12-Sandwich-ELISA-H00001639-M01-img0001.jpg "Sandwich ELISA: Dynactin Subunit 1/DCTN1 Antibody (1E12) [H00001639-M01] - Detection limit for recombinant GST tagged DCTN1 is 0.1 ng/ml as a capture antibody.")
![Western Blot: Dynactin Subunit 1/DCTN1 Antibody (2E4-1C2) [H00001639-M02] - Detection against Immunogen (47.52 KDa) .](http://images.novusbio.com/fullsize/Dynactin-Subunit-1-DCTN1-Antibody-2E4-1C2-Western-Blot-H00001639-M02-img0001.jpg "Western Blot: Dynactin Subunit 1/DCTN1 Antibody (2E4-1C2) [H00001639-M02] - Detection against Immunogen (47.52 KDa) .")
