| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-594SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05119-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22424-50ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP3-50166AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-50166AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-50166AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-50166AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-50166AFP680 | |||
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: FFPE. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4 degrees Celsius for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-NB600-594-img0018.jpg "Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Tissue: right lobe of the liver section. A:Central Vein (CV) fibrosis, B: Non-fibrotic CV, C: Perisinusodial fibrosis, D: Non-fibrotic area, E: Protat tract fibrosis, F: Septal fibrosis (arrow). Fixation: FFPE. Antigen retrieval: not required. Primary antibody: Anti-collagen type I at 1:500 for 4 degrees Celsius for 24hr. Secondary antibody: Peroxidase biotin-streptavidin rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Anti-collagen type III is intra and extracellular. Staining: 3.3'-diaminobenzidine tetrahydrochloride was used as the chromogen. Nuclei were counterstained purple with hematoxylin.")
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Lane 1: Human Collagen III Load: 100 ng per lane Primary antibody: Collagen III Antibody at 1:1000 o/n at 4C Secondary antibody: DyLight 649 Goat anti-rabbit at 1:20,000 for 30 min at RT Block: incubated with blocking buffer for 30 min at RT Predicted/Observed size: 138 kDa/138 kDa.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NB600-594-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human primary ventricular cardiac fibroblasts were stained with anti-Collagen III antibody. Cells were cultured for 3 days in DMEM with 10% fetal calf serum. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-594-img0012.jpg)
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Staining in FFPE sections of human skin(left, dermis) with moderate to strong red staining and testis (right) where strong staining was observed within connective tissue between seminiferous tubules. The antibody showed strong extracellular staining within connective tissues across many organs with minimal background staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C. 20 minutes for antigen retrieval.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-NB600-594-img0016.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Immunodetection of ID3, COL1A2 and COL3A1 in human dermal fibroblasts subjected to treatment with or without cANGPTL4 (12 ug/mL) in the presence of TGFB1 (10 ng/mL). B-tubulin, as loading control, was from the same samples. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-017-05869-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NB600-594-img0023.jpg)
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Livers were harvested at 9 wpi, formalin-fixed, and then cryosectioned and stained for markers of inflammation and liver fibrosis. Maximum intensity projections of 12-17 um thick liver sections with immunofluorescently labeled nuclei (DAPI white) alpha-smooth muscle actin NBP2-34760APC (yellow), CD45 (red) and Collagen I NB600-408 (top) or Collagen III/COL3A1 NB600-594 (bottom) (blue) in the liver of uninfected or 9 wpi WT mice. Scale bars represent 50 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32973293/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen III alpha 1-COL3A1 Antibody-Immunohistochemistry-NB600-594-img0024.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Western blot for COL3A1 protein with PVDF protein staining to control for protein loading. Image collected and cropped by CiteAb from the following publication (mdpi.com/2076-3921/6/4/87), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NB600-594-img0019.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen III alpha 1 expression in HT-1080 cells. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-594-img0008.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NB600-594-img0013.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Human lung tissue](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NB600-594-img0014.jpg)
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen III (k, positive staining indicated by arrows) immunohistochemistry staining in 13 weeks old male offspring. Left panel = Sham, Middle panel = SE (cigarette smoke exposure), Right panel = SE+MQ (SE with MitoQ supplementation) bar = 50 um. Image collected and cropped by CiteAb from the following publication (mdpi.com/2072-6643/11/7/1669) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-NB600-594-img0020.jpg)
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen I and collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated and control mice were stained for collagen I or for collagen III and imaged using fluorescent microscopy. (a) Representative images (100 x 100 um) of each mouse strain and treatment group are provided for collagen I and collagen III immunofluorescence. Collagen I antibody (NB600-450, RRID:AB_522923) and Collagen III antibody (NB600-594, RRID:AB_10001330). Image collected and cropped by CiteAb from the following publication (onlinelibrary.wiley.com/doi/abs/10.14814/phy2.14377) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-NB600-594-img0021.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - SCE attenuates myocardial fibrosis in heart tissue after MI. (a) RT-qPCR analysis for mRNA expression of collagen type I alpha 1 (Col1a1), collagen type III alpha 1 (Col3a1), actin, alpha 2 smooth muscle (Acta2), Mmp2, & Mmp9 in the border area (BA) & remote area (RA) 7 days, 14 days, & 21 days after MI. (b) Immunoblot analysis of protein expressions of alpha -SMA, collagen I, & collagen III in RA & BA in the heart tissues 7 days, 14 days, & 21 days after MI. Data are from three independent experiments ((a), mean ± SEM) or are representative of three independent experiments with similar results (b). ∗P < 0.05 & ∗∗P < 0.01, ANOVA with LSD t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31275414), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-594_rabbit-polyclonal-collagen-iii-alpha-1-col3a1-antibody-31020241534373.jpg)
![Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Collagen I & collagen III accumulate in C57BL/6 mice in response to radiation. Bladder strips from irradiated & control mice were stained for collagen I or for collagen III & imaged using fluorescent microscopy. (a) Representative images (100 x 100 µm) of each mouse strain & treatment group are provided for collagen I & collagen III immunofluorescence. (b–c) Percentage of tissue‐positive for collagen I or collagen III staining. Collagen I & III density is significantly elevated in C57BL/6 mice, but not in the C3H or BALB/c strains. Results are mean ± SD of n = 3–6 mice. Dashed line: ANOVA; Full line: multiple t‐test. **p < .01, *** p < .001 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32109348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-594_rabbit-polyclonal-collagen-iii-alpha-1-col3a1-antibody-310202415371941.jpg)
![Immunohistochemistry: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - Number of collagen I‐expressing cells is increased in irradiated C57BL/6 detrusor. Bladder strips from irradiated & control mice were stained for collagen I or for collagen III mRNA & counterstained with methyl green. Collagen I & III mRNA (pink) is apparent in both control & irradiated bladder sections in all three mouse strains. The number of collagen I & III‐producing cells in the detrusor is represented in the bar graphs. The number of collagen I‐expressing cells is significantly elevated in C57BL/6 mice after irradiation. Dashed line separates the urothelium (U) & lamina propria (LP) from the detrusor muscle (D). Results are mean ± SD of n = 3–4 mice. Scale bar = 100 µm. op ≤ .08, * p < .05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32109348), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-594_rabbit-polyclonal-collagen-iii-alpha-1-col3a1-antibody-310202415395941.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NB600-594] - SCE attenuates fibrotic responses in CFs by inhibiting TGF-beta /Smad3 signaling activation. (a) RT-qPCR analysis of mRNA expression for genes of Col1a1, Col3a1, & Acta2 in cardiac fibroblasts (CFs) pretreated with different concentrations of SCE (5 μl/ml, 10 μl/ml, & 15 μl/ml) followed by TGF-beta stimulation (20 ng/ml) for 24 h. (b) RT-qPCR analysis of mRNA expression for genes of Col1a1, Col3a1, & Acta2 in CFs pretreated with SCE (10 μl/ml) followed by TGF-beta stimulation (20 ng/ml) for 12 h & 24 h. (c) RT-qPCR analysis of mRNA expression for genes of Smad2, Smad3, & Smad7 in CFs pretreated with SCE (10 μl/ml) followed by TGF-beta stimulation (20 ng/ml) for 12 h & 24 h. (d) Immunoblot analysis of expression of Smad2, Smad3, & Smad7 in CFs pretreated with SCE (10 μl/ml) followed by TGF-beta stimulation (20 ng/ml) for 24 h & 36 h. Data are from three independent experiments ((a)–(c), mean ± SEM) or are representative of three independent experiments with similar results (d). ∗P < 0.05 & ∗∗P < 0.01, Student's t-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31275414), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb600-594_rabbit-polyclonal-collagen-iii-alpha-1-col3a1-antibody-31020241616022.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119] - Western blot analysis on total proteins extracted from cells cultured with L-PRP or P-PRP. Collagen I protein band was robust in the P-PRP-treated group; collagen III alpha 1 protein band was more abundant in the L-PRP-treated cells. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/173) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-1E7-D7-Col3-BSA-Free-Western-Blot-NBP1-05119-img0003.jpg "<b>Biological Strategies Validation. </b>Western Blot: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119] - Western blot analysis on total proteins extracted from cells cultured with L-PRP or P-PRP. Collagen I protein band was robust in the P-PRP-treated group; collagen III alpha 1 protein band was more abundant in the L-PRP-treated cells. Image collected and cropped by CiteAb from the following publication (//stemcellres.com/content/6/1/173) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody (1E7-D7/Col3) - BSA Free [NBP1-05119] - Human bronchus tissue](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-1E7-D7-Col3-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05119-img0002.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Human PAPILLARY CA_STROMAL CELLS, using Collagen III antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15946-img0008.jpg "Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Human PAPILLARY CA_STROMAL CELLS, using Collagen III antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![<b>Genetic Strategies Validation. </b>Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP2-15946] - Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15946_rabbit-polyclonal-collagen-iii-alpha-1-col3a1-antibody-1610202419414522.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3], C-term (NBP2-15946) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15946_rabbit-polyclonal-collagen-iii-alpha-1-col3a1-antibody-western-blot-2611202519482.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Western-Blot-NBP3-15309-img0004.jpg "Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunofluorescence analysis of NIH/3T3 cells using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunocytochemistry-Immunofluorescence-NBP3-15309-img0002.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Western blot analysis of extracts of various cell lines, using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Western-Blot-NBP3-15309-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunofluorescence analysis of RD cells using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunocytochemistry-Immunofluorescence-NBP3-15309-img0001.jpg)
![Immunoprecipitation: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [NBP3-15309] - Immunoprecipitation analysis of 300ug extracts of HepG2 cells using 3ug Collagen III alpha 1/COL3A1 antibody (NBP3-15309). Western blot was performed from the immunoprecipitate using Collagen III alpha 1/COL3A1 antibody (NBP3-15309) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-6Y9C6-Immunoprecipitation-NBP3-15309-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (6Y9C6) [Collagen III alpha 1/COL3A1] - Confocal imaging of HeLa cells using Collagen III alpha 1/COL3A1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15309_rabbit-collagen-iii-alpha-1-col3a1-mab-6y9c6-52202517273527.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Analysis in human cell lines HeLa and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Western-Blot-NBP1-84007-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Analysis in human cell lines HeLa and SK-MEL-30. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0011.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human colorectal cancer shows weak to moderate cytoplasmic positivity in stromal cells.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0009.jpg)
![Immunohistochemistry-Paraffin: Collagen III alpha 1/COL3A1 Antibody [NBP1-84007] - Staining of human skin shows weak to moderate cytoplasmic positivity in fibroblasts.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84007-img0010.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - Western Blot of Collagen III alpha 1/COL3A1. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 at 4C. Secondary antibody: DyLight(TM) 649 Goat anti-rabbit at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Native-Protein-Western-Blot-NBP1-97267-img0004.jpg "Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - Western Blot of Collagen III alpha 1/COL3A1. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 at 4C. Secondary antibody: DyLight(TM) 649 Goat anti-rabbit at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.")
![SDS-Page: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - SDS-PAGE Results of Collagen III alpha 1/COL3A1 Native protein. Lane 1: Collagen III alpha 1/COL3A1 Native protein Reduced [1.0ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Collagen III alpha 1/COL3A1 Native protein Non-Reduced [1.0ug]. 4-20% Gel Coomassie Stained. Observed: alpha1 bands.](http://images.novusbio.com/fullsize/Collagen III alpha 1-COL3A1 Native Protein-SDS-Page-NBP1-97267-img0005.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25419_rabbit-collagen-iii-alpha-1-col3a1-mab-hl1906-azide-bsa-free-2120249371516.jpg "Western Blot: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Collagen III alpha 1/COL3A1 Antibody (HL1906) - Azide and BSA Free [NBP3-25419] - Collagen III antibody [HL1906] detects Collagen III protein at cytoplasmic vesicle by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in ice-cold MeOH for 5 min. Green: Collagen III stained by Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25419_rabbit-collagen-iii-alpha-1-col3a1-mab-hl1906-azide-bsa-free-2120249432898.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [HL1906] (NBP3-25419) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25419_rabbit-collagen-iii-alpha-1-col3a1-mab-hl1906-azide-bsa-free-western-blot-261120251838153.jpg)
![Western Blot: Collagen III alpha 1/COL3A1 Antibody (SR1186) - BSA Free [NBP3-22424] - Western blot analysis of (1) A431 cell lysate; (2) MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22424_rabbit-collagen-iii-alpha-1-col3a1-mab-sr1186-103202523472628.jpg "Western Blot: Collagen III alpha 1/COL3A1 Antibody (SR1186) - BSA Free [NBP3-22424] - Western blot analysis of (1) A431 cell lysate; (2) MCF7 cell lysate.")
![SDS-Page: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97244] - SDS-PAGE Results of Collagen III alpha 1/COL3A1 Native protein. Lane 1: Collagen III alpha 1/COL3A1 Native protein Reduced (7.0ug).Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Collagen III alpha 1/COL3A1 Native protein Non-Reduced (7.0ug). 7.5% Gel, Coomassie Stained. Observed MW: Type III trimer, dimer, monomer.](http://images.novusbio.com/fullsize/Collagen-III-alpha-1-COL3A1-Native-Protein-SDS-Page-NBP1-97244-img0001.jpg "SDS-Page: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97244] - SDS-PAGE Results of Collagen III alpha 1/COL3A1 Native protein. Lane 1: Collagen III alpha 1/COL3A1 Native protein Reduced (7.0ug).Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Collagen III alpha 1/COL3A1 Native protein Non-Reduced (7.0ug). 7.5% Gel, Coomassie Stained. Observed MW: Type III trimer, dimer, monomer.")