| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4099-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33660-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4099-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB40991-SP | |||
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Analysis in human esophagus and placenta tissues. Corresponding Chymase/CMA1/Mast Cell Chymase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Analysis in human esophagus and placenta tissues. Corresponding Chymase/CMA1/Mast Cell Chymase RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Aged control whole mount choroid immunolabeled for (A) UEA lectin (blue), (B) tryptase (red), and (C) chymase (green). The vast majority of human choroidal MCs are of the MCT phenotype (tryptase+/chymase-). Only a small percentage of MCs are MCtc (tryptase+/chymase+) (arrows). Scale bar: 30 um. Image collected and cropped by CiteAb from the following publication (//iovs.arvojournals.org/article.aspx?doi=10.1167/iovs.17-22989), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33660-img0013.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0012.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0008.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human colon shows strong cytoplasmic positivity in mast cell.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0009.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human esophagus shows strong cytoplasmic positivity in mast cell.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0010.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Staining of human lymph node shows strong cytoplasmic positivity in mast cell.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-33660-img0011.jpg)
![Immunocytochemistry/ Immunofluorescence: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-33660] - Aged control whole mount choroid immunolabeled for (A) UEA lectin (blue), (B) tryptase (red), & (C) chymase (green). The vast majority of human choroidal MCs are of the MCT phenotype (tryptase+/chymase−). Only a small percentage of MCs are MCtc (tryptase+/chymase+) (arrows). Scale bar: 30 μm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29164232), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33660_rabbit-polyclonal-chymase-cma1-mast-cell-chymase-antibody-310202415291960.jpg)
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Western-Blot-NBP2-75441-img0006.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of Chymase/CMA1/Mast Cell on different lysates with Rabbit anti-Chymase/CMA1/Mast Cell antibody at 1/500 dilution. Lane 1: Mouse spleen tissue lysate Lane 2: Rat spleen tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 2 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0008.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human tonsil tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0004.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Human small intestine tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0005.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human skin tissue using anti-Mast Cell Chymase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0003.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (JB74-32) [NBP2-75441] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Chymase/CMA1/Mast Cell Chymase antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-JB74-32-Immunohistochemistry-Paraffin-NBP2-75441-img0007.jpg)
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with NBP2-27551 (0.5 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag (1:3000) in lane C. Mock-transfected HEK293 probed with NBP2-27551 (1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Western-Blot-NBP2-27551-img0002.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - HEK293 lysate (10 ug protein in RIPA buffer) over expressing Human CMA1 with DYKDDDDK tag probed with NBP2-27551 (0.5 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag (1:3000) in lane C. Mock-transfected HEK293 probed with NBP2-27551 (1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of paraffin embedded Human Tonsil (Mast cells). Antibody at 1.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-27551-img0006.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of human skin Epidermis shows detection on the apical side of the basal cell layer.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-27551-img0004.jpg)
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody [NBP2-27551] - Staining of paraffin embedded Human Testis (Mast cells). Antibody at 1.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-Immunohistochemistry-Paraffin-NBP2-27551-img0005.jpg)
![N/A Chymase/CMA1/Mast Cell Chymase [Biotin]](http://images.novusbio.com/fullsize2/dy4099-05_human-chymase-cma1-duoset-elisa-5-plate-2052021112751.jpg "N/A Chymase/CMA1/Mast Cell Chymase [Biotin]")
![Immunohistochemistry: Chymase/CMA1/Mast Cell Chymase Antibody (CC1) [NB100-693] - Staining of a human tonsil cross section](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-CC1-Immunohistochemistry-NB100-693-img0004.jpg "Immunohistochemistry: Chymase/CMA1/Mast Cell Chymase Antibody (CC1) [NB100-693] - Staining of a human tonsil cross section")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (CC1) [NB100-693] - Formalin fixed paraffin embedded human tonsil stained with Mast Cell Chymase antibody.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-CC1-Immunohistochemistry-Paraffin-NB100-693-img0002.jpg)
![Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389] - Analysis of extracts of various cell lines, using Chymase/CMA1/Mast Cell Chymase (CMA1) Rabbit mAb (NBP3-15389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-2O4P8-Western-Blot-NBP3-15389-img0002.jpg "Western Blot: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389] - Analysis of extracts of various cell lines, using Chymase/CMA1/Mast Cell Chymase (CMA1) Rabbit mAb (NBP3-15389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Chymase/CMA1/Mast Cell Chymase Antibody (2O4P8) [NBP3-15389] - Human appendix using Chymase/CMA1/Mast Cell Chymase (CMA1) Rabbit mAb (NBP3-15389) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Chymase-CMA1-Mast-Cell-Chymase-Antibody-2O4P8-Immunohistochemistry-Paraffin-NBP3-15389-img0001.jpg)
![ELISA: Mouse Chymase/CMA1/Mast Cell Chymase ELISA Kit (Colorimetric) [NBP2-75390] - Samples were spiked with high concentrations of Mouse Chymase/CMA1/Mast Cell Chymase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75390_mouse-chymase-cma1-mast-cell-chymase-elisa-kit-color-13220241685376.jpg "ELISA: Mouse Chymase/CMA1/Mast Cell Chymase ELISA Kit (Colorimetric) [NBP2-75390] - Samples were spiked with high concentrations of Mouse Chymase/CMA1/Mast Cell Chymase and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Chymase/CMA1/Mast Cell Chymase ELISA Kit (Colorimetric) [NBP2-75390] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75390_mouse-chymase-cma1-mast-cell-chymase-elisa-kit-color-4320248574342.jpg)
![Western Blot: Mast Cell Chymase Overexpression Lysate (Adult Normal) [NBL1-09293] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mast Cell Chymase.](http://images.novusbio.com/fullsize/Mast-Cell-Chymase-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09293-img0002.jpg "Western Blot: Mast Cell Chymase Overexpression Lysate (Adult Normal) [NBL1-09293] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Mast Cell Chymase.")