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![Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize2/CHD4-Antibody-(CHD4-3F2-4)-Flow-Cytometry-NBP2-50163-img0001.jpg "Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Western Blot: CHD4 Antibody (3F2/4) [NBP3-07505] - Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).](http://images.novusbio.com/fullsize2/CHD4-Antibody-(3F2-4)-Western-Blot-NBP3-07505-img0003.jpg "Western Blot: CHD4 Antibody (3F2/4) [NBP3-07505] - Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody (3F2/4) [NBP3-07505] - Confocal immunofluorescence image of MCF-7 cells using CHD4 Mouse Monoclonal Antibody (3F2/4) stained in Green (CF488) and Phalloidin is used to label the membranes.](http://images.novusbio.com/fullsize2/CHD4-Antibody-(3F2-4)-Immunocytochemistry-Immunofluorescence-NBP3-07505-img0001.jpg)
![Immunohistochemistry-Paraffin: CHD4 Antibody (3F2/4) [NBP3-07505] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CHD4 Mouse Monoclonal Antibody (3F2/4).](http://images.novusbio.com/fullsize2/CHD4-Antibody-(3F2-4)-Immunohistochemistry-Paraffin-NBP3-07505-img0002.jpg)
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/CHD4-Antibody-(4H4)-Western-Blot-H00001108-M01-img0009.jpg "Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize2/CHD4-Antibody-(4H4)-Immunohistochemistry-Paraffin-H00001108-M01-img0006.jpg)
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - CHD4 monoclonal antibody (M01), clone 4H4 Analysis of CHD4 expression in Hela S3 NE.](http://images.novusbio.com/fullsize2/CHD4-Antibody-(4H4)-Western-Blot-H00001108-M01-img0008.jpg)
![Sandwich ELISA: CHD4 Antibody (4H4) [H00001108-M01] - Detection limit for recombinant GST tagged CHD4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/CHD4-Antibody-(4H4)-Sandwich-ELISA-H00001108-M01-img0007.jpg)
![Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize2/CHD4-Antibody-Western-Blot-NB100-57519-img0005.jpg "Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: CHD4 Antibody [NB100-57519] - Detection of Human CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody NB100-57519 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize2/CHD4-Antibody-Western-Blot-NB100-57519-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57519] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57519 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57520, NB100-57521), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). For blotting immunoprecipitated CHD4, NBP2-77358 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize2/CHD4-Antibody-Immunoprecipitation-NB100-57519-img0006.jpg)
![Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.](http://images.novusbio.com/fullsize2/CHD4-Antibody-Western-Blot-NB100-57521-img0004.jpg "Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57521] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57521 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize2/CHD4-Antibody-NB100-57521-img0003.jpg)
![Western Blot: CHD4 Antibody (BLR066G) [NBP2-77358] - Antibody: Rabbit anti-CHD4 recombinant monoclonal [BLR066G] (NBP2-77358). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize2/CHD4-Antibody-(BLR066G)-Western-Blot-NBP2-77358-img0002.jpg "Western Blot: CHD4 Antibody (BLR066G) [NBP2-77358] - Antibody: Rabbit anti-CHD4 recombinant monoclonal [BLR066G] (NBP2-77358). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![Western Blot: CHD4 Antibody (BLR066G) [NBP2-77358] - Antibody: Rabit anti-CHD4 recombinant monoclonal [BLR066G] (NBP2-77358). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize2/CHD4-Antibody-(BLR066G)-Western-Blot-NBP2-77358-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody (BLR066G) [NBP2-77358] - Detection of human CHD4 by WB of immunoprecipitates from HEK293T lysate. Antibodies: Rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358) and rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520, NB100-57521).](http://images.novusbio.com/fullsize2/CHD4-Antibody-(BLR066G)-Immunoprecipitation-NBP2-77358-img0003.jpg)
![Western Blot: CHD4 Antibody [NB100-57520] - Detection of Human and Mouse CHD4/Mi2 beta on HeLa whole cell lysate using NB100-57520. CHD4/Mi2 beta was also IPed by rabbit anti-CHD4/Mi2 beta antibodies NB100-57519 and NB100-57521.](http://images.novusbio.com/fullsize2/CHD4-Antibody-Western-Blot-NB100-57520-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CHD4 Antibody [NB100-57520] - Detection of Human and Mouse CHD4/Mi2 beta on HeLa whole cell lysate using NB100-57520. CHD4/Mi2 beta was also IPed by rabbit anti-CHD4/Mi2 beta antibodies NB100-57519 and NB100-57521.")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP1-82651] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/CHD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82651-img0004.jpg "Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP1-82651] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: CHD4 Antibody [NBP1-82651] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/CHD4-Antibody-Immunohistochemistry-Paraffin-NBP1-82651-img0001.jpg)
![Western Blot: CHD4 Antibody [NBP2-99099] - Anti-CHD4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 218 kDa](http://images.novusbio.com/fullsize2/CHD4-Antibody-Western-Blot-NBP2-99099-img0002.jpg "Western Blot: CHD4 Antibody [NBP2-99099] - Anti-CHD4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: 293T Whole Cell Lysate Lane C: NIH3T3 Whole Cell Lysate Lane D: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 218 kDa")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP2-99099] - Immunofluorescence staining of CHD4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CHD4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize2/CHD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99099-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP2-99098] - Immunofluorescence staining of CHD4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CHD4 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.](http://images.novusbio.com/fullsize2/CHD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-99098-img0001.jpg "Immunocytochemistry/Immunofluorescence: CHD4 Antibody [NBP2-99098] - Immunofluorescence staining of CHD4 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human CHD4 polyclonal antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to nucleus.")
![Immunohistochemistry-Paraffin: CHD4 Antibody [NBP2-99098] - Immunochemical staining of human CHD4 in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize2/CHD4-Antibody-Immunohistochemistry-Paraffin-NBP2-99098-img0002.jpg)
![Western Blot: CHD4 Antibody (SR1411) [NBP3-21737] - Western blot analysis using NBP3-21737 in 293T cell lysate.](http://images.novusbio.com/fullsize/nbp3-21737_rabbit-chd4-mab-sr1411-57202316582186.jpg "Western Blot: CHD4 Antibody (SR1411) [NBP3-21737] - Western blot analysis using NBP3-21737 in 293T cell lysate.")
