HAF007). For additional reference, Recombinant Human Fibulin 5 (Catalog # 3095-FB) (5 ng/lane) was included. A specific band was detected for Fibulin 5/DANCE at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human heart tissue, human lung tissue, and human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Fibulin 5/DANCE Monoclonal Antibody (Catalog # MAB3095) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Fibulin 5 (Catalog # 3095-FB) (5 ng/lane) was included. A specific band was detected for Fibulin 5/DANCE at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3095-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90101-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90100-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21755-50ul | |||
![Western Blot: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-2424-Western-Blot-NBP2-76929-img0001.jpg "Western Blot: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - ICC staining Fibulin 5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-2424-Immunocytochemistry-Immunofluorescence-NBP2-76929-img0004.jpg)
![Immunohistochemistry: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Mouse ovary tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-2424-Immunohistochemistry-NBP2-76929-img0006.jpg)
![Flow Cytometry: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Flow cytometric analysis of SiHa cells with Fibulin 5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-2424-Flow-Cytometry-NBP2-76929-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - ICC staining Fibulin 5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-2424-Immunocytochemistry-Immunofluorescence-NBP2-76929-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - ICC staining Fibulin 5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-2424-Immunocytochemistry-Immunofluorescence-NBP2-76929-img0003.jpg)
![Immunohistochemistry: Fibulin 5/DANCE Antibody (2424) [NBP2-76929] - Rat testis tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-2424-Immunohistochemistry-NBP2-76929-img0005.jpg)
![Western blot analysis on different lysates with NBP2-76929 at 1/1,000 dilution. Lane 1: SK-OV-3 (human ovarian cancer cells) cell tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-76929, 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50.2 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp2-76929_-western-blot-639204597790458466.jpg)
![Western blot analysis on different lysates with NBP2-76929 at 1/1,000 dilution. Lane 1: JAR (human choriocarcinoma cells) cell tissue lysate Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-76929, 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50.2 kDa Observed band size: 50 kDa](http://images.novusbio.com/fullsize/nbp2-76929_-western-blot-639204598087485485.jpg)
![Western Blot: Fibulin 5/DANCE Antibody (1G6A4) [NBP1-28890] - Analysis using FBLN5 mouse mAb against truncated FBLN5-His recombinant protein.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-1G6A4-Western-Blot-NBP1-28890-img0001.jpg "Western Blot: Fibulin 5/DANCE Antibody (1G6A4) [NBP1-28890] - Analysis using FBLN5 mouse mAb against truncated FBLN5-His recombinant protein.")
![Immunohistochemistry-Paraffin: Fibulin 5/DANCE Antibody (1G6A4) [NBP1-28890] - Analysis of human colon cancer (left) and breast cancer (right) showing cytoplasmic localization with DAB staining using FBLN5 mouse mAb.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-1G6A4-Immunohistochemistry-Paraffin-NBP1-28890-img0002.jpg)
![ELISA: Human Fibulin 5/DANCE ELISA Kit (Colorimetric) [NBP3-31645] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31645_human-fibulin-5-dance-elisa-kit-colorimetric-206202415361349.png "ELISA: Human Fibulin 5/DANCE ELISA Kit (Colorimetric) [NBP3-31645] - Standard Curve Reference")
![Western Blot: Fibulin 5/DANCE Antibody [NB100-53783] - (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-Western-Blot-NB100-53783-img0003.jpg "Western Blot: Fibulin 5/DANCE Antibody [NB100-53783] - (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![ELISA: Human Fibulin 5/DANCE - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31646] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31646_human-fibulin-5-dance-ready-to-use-elisa-kit-color-206202415311385.png "ELISA: Human Fibulin 5/DANCE - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31646] - Standard Curve Reference")
![Western Blot: Fibulin 5/DANCE Antibody [NBP2-16495] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Fibulin-5-DANCE-Antibody-Western-Blot-NBP2-16495-img0002.jpg "Western Blot: Fibulin 5/DANCE Antibody [NBP2-16495] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: Fibulin 5/DANCE Antibody (SR1879) [NBP3-21755] - Western blot analysis using NBP3-21755 in mouse heart lysate.](http://images.novusbio.com/fullsize/nbp3-21755_rabbit-fibulin-5-dance-mab-sr1879-57202316494239.jpg "Western Blot: Fibulin 5/DANCE Antibody (SR1879) [NBP3-21755] - Western blot analysis using NBP3-21755 in mouse heart lysate.")
![Western Blot: Fibulin 5 Overexpression Lysate (Adult Normal) [NBL1-10609] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Fibulin 5.](http://images.novusbio.com/fullsize/Fibulin-5-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10609-img0002.jpg "Western Blot: Fibulin 5 Overexpression Lysate (Adult Normal) [NBL1-10609] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Fibulin 5.")
