Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92695SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47785-0.1mg | |||
| IHC-Prediluted | NBP2-48331 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-49701SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP2-46876 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0300A488-100 | |||
| Alexa Fluor 546 | DDX0300A546-100 | |||
| Alexa Fluor 647 | DDX0300A647-100 | |||
| Biotin | DDX0300B-100 | |||
![Western Blot: CEP290 Antibody [NB100-86991] - Representative examples of immunoblot images of purified olfactory cilia. Three distinct cilia preparations each from LRBA-KO and WT mice (age, 4 weeks) were blotted in parallel (10 ug protein each), along with an OE and a brain sample (50 ug protein each), all shown at the same exposure. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-08543-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CEP290-Antibody-Western-Blot-NB100-86991-img0011.jpg "Western Blot: CEP290 Antibody [NB100-86991] - Representative examples of immunoblot images of purified olfactory cilia. Three distinct cilia preparations each from LRBA-KO and WT mice (age, 4 weeks) were blotted in parallel (10 ug protein each), along with an OE and a brain sample (50 ug protein each), all shown at the same exposure. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-08543-4) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: CEP290 Antibody [NB100-86991] - NBF-fixed asynchronous HeLa cells. Antibody used at a dilution of 1:500.](http://images.novusbio.com/fullsize/CEP290-Antibody-Immunocytochemistry-Immunofluorescence-NB100-86991-img0007.jpg)
![Western Blot: CEP290 Antibody [NB100-86991] - Species: Mouse, Cell line: Brain homogenate, Primary antibody dilution: 1:400. Band detected at 300KDa. This image has been provided by Manfred W. Kilimann, MD, PhD, Professor in Molecular Cell Biology, Uppsala University.](http://images.novusbio.com/fullsize/CEP290-Antibody-Western-Blot-NB100-86991-img0008.jpg)
![Western Blot: CEP290 Antibody [NB100-86991] - Detection of human CEP290 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CEP290 antibody NB100-86991 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 second (B).](http://images.novusbio.com/fullsize/CEP290-Antibody-Western-Blot-NB100-86991-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16908-img0041.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody diluted at 1:500.Blue: Fluoroshield with DAPI .")
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - THP-1 cell. Black: Unlabelled sample was used as a control. Red: AIF-1:Iba1 Antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0032.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0035.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-16908] - Mouse brain. Iba1 stained by Iba1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-16908-img0036.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP2-16908] - Primary murine microglia cells, staining with AIF-1:Iba1 Antibody using 1. 0 ug per 4 x 10^5 cells. NBP2-16908 (blue), Rabbit IgG (green) Unstained (red).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP2-16908-img0014.jpg)
![Western Blot: AIF-1/Iba1 Antibody [NBP2-16908] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with AIF-1/Iba1 antibody (NBP2-16908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16908_rabbit-polyclonal-aif-1-iba1-antibody-910202419593573.jpg)
![Western Blot: ER beta/NR3A2 Antibody (6A12) [NB200-303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12]. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ER-beta-NR3A2-Antibody-6A12-Western-Blot-NB200-303-img0001.jpg "Western Blot: ER beta/NR3A2 Antibody (6A12) [NB200-303] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12]. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Western-Blot-NBP1-92695-img0005.jpg "Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of whole brain lysates and nuclear inreached extract from whole brain using mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat brain nuclear extract, [4] mouse brain, [5] mouse brain nuclear extract. Strong band at 43 kDa mark corresponds to TDP43 protein.")
![Immunocytochemistry/Immunofluorescence: TARDBP Antibody (3H8) [NBP1-92695] - NBP1-92695 was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP (NBP1-05198, green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDBP (3H8) antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TARDBP and blue due to their content of DNA, stained blue with DAPI.](http://images.novusbio.com/fullsize/TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0003.jpg)
![Immunohistochemistry Free-Floating: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Analysis of rat hippocampus section stained with mouse mAb to TDP43, NBP1-92695, dilution 1:2,000 in red, and costained with chicken pAb to GFAP,, dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The TDP43 protein is concentrated in the nuclei of hippocampal neurons, while the GFAP antibody stains the intermediate filament network of astroglial cells.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunocytochemistry-Immunofluorescence-NBP1-92695-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - Lysates of HAP1 (WT) and TDP-43/TARDBP (KO) were prepared, and 50 ug of protein was processed for western blot with the TDP-43/TARDBP antibody. The Ponceau stained transfers of the blot is shown (right). Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0006.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 lysates were prepared, and immunoprecipitation was performed using 2.0 ug of the TDP-43/TARDBP antibody pre-coupled to Dynabeads protein G. Sample was washed and processed for immunoblot with the TDP-43/TARDBP antibody. The Ponceau stained transfer of the blot is shown. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Immunoprecipitation-NBP1-92695-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (3H8) [NBP1-92695] - HAP1 (WT) and TDP-43/TARDBP (KO) cells were labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio in a 96-well plate with glass bottom. Cells were stained with the TDP-43/TARDBP antibody and with the corresponding Alexa-fluor 555 coupled secondary antibody including DAPI. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined on both channels with green and magenta dashed line, respectively. Antibody dilution used: NBP1-92695 at 1/1000. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-3H8-Knockout-Validated-NBP1-92695-img0008.jpg)
![Western Blot: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Western Blot Analysis of human THP-1 cell lysate using CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10)](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-+-C31-7-+-C31-10-Western-Blot-NBP2-44342-img0003.jpg "Western Blot: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Western Blot Analysis of human THP-1 cell lysate using CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10)")
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Formalin-fixed, paraffin-embedded Angiosarcoma stained with CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-+-C31-7-+-C31-10-Immunohistochemistry-Paraffin-NBP2-44342-img0002.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Colon Carcinoma stained with CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-3-+-C31-7-+-C31-10-Immunohistochemistry-Paraffin-NBP2-44342-img0001.jpg)
![Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3 + C31.7 + C31.10) [NBP2-44342] - Cholangiocellular carcinoma (liver cancer tissue) stained with ATTO550 conjugated CD31 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-44342_mouse-monoclonal-cd31-pecam-1-antibody-c31-3-c31-7-c31-10-17720241044653.jpg)
![Western Blot: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/FUS-Antibody-Knockout-Validated-NB100-562-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Western blot shows lysates of HEK293 human embryonic kidney parental cell line and FUS knockout (KO) HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human FUS Polyclonal Antibody (Catalog # NB100-562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for FUS at approximately 75 kDa (as indicated) in the parental HEK293 celll line, but is not detectable in the knockout HEK293 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Detection of human FUS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-FUS antibody NB100-562 (lot NB100-562-2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. FUS was also immunoprecipitated by a previous lot (lot NB100-562-1) of this antibody. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-562-img0010.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody [NB100-562] - Section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-FUS used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/FUS-Antibody-Immunohistochemistry-Paraffin-NB100-562-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: FUS Antibody [NB100-562] - Detection of human FUS on HeLa whole cell lysate using NB100-562. For IP in B, rabbit anti-FUS antibodies NB100-560, NB100-561, NB100-562 and NB100-565 were used.](http://images.novusbio.com/fullsize/FUS-Antibody-Western-Blot-NB100-562-img0004.jpg)
![Western Blot: Alix Antibody [NBP1-49701] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ALIX antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ALIX was also immunoprecipitated by rabbit anti-ALIX antibody NBP1-49700, which recognizes an upstream epitope.](http://images.novusbio.com/fullsize/Alix-Antibody-Western-Blot-NBP1-49701-img0001.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Alix Antibody [NBP1-49701] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ALIX antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. ALIX was also immunoprecipitated by rabbit anti-ALIX antibody NBP1-49700, which recognizes an upstream epitope.")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-49701] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Alix Antibody (NBP1-49701) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49701-img0005.jpg)
![Immunohistochemistry-Paraffin: Alix Antibody [NBP1-49701] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- ALIX used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Alix-Antibody-Immunohistochemistry-Paraffin-NBP1-49701-img0002.jpg)
![Flow Cytometry: Alix Antibody [NBP1-49701] - An intracellular stain was performed on NIH3T3 cells with Alix Antibody NBP1-49701 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Alix-Antibody-Flow-Cytometry-NBP1-49701-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Alix Antibody [NBP1-49701] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with Alix Antibody (NBP1-49701) at 1 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Alix-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49701-img0004.jpg)
![Flow Cytometry: Alix Antibody [NBP1-49701] - An intracellular stain was performed on HeLa cells with Alix Antibody NBP1-49701 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/Alix-Antibody-Flow-Cytometry-NBP1-49701-img0006.jpg)
![Simple Western: Alix Antibody - BSA Free [NBP1-49701] - Alix Antibody [NBP1-49701] - Simple Western lane view shows lysates of Exosome Standards (A549 cell line) (NBP3-11645), exosome standards (HT29 cell line) (NBP3-11685), and COLO 205 human colorectal adenocarcinoma cell line whole cell lysate (WCL), loaded at 0.2 mg/mL. A specific band was detected for Alix antibody (NBP1-49701) at approximately 106 kDa (as indicated) using 1:10 of Alix antibody. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Alix-Antibody-Simple-Western-NBP1-49701-img0003.jpg)
![Western Blot: PARG1 Antibody [NBP1-05989] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. PARG1 was also immunoprecipitated by rabbit anti-PARG1 antibody NBP1-05988.](http://images.novusbio.com/fullsize/PARG1-Antibody-Western-Blot-NBP1-05989-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PARG1 Antibody [NBP1-05989] - Whole cell lysate from HeLa, 293T, and mouse NIH3T3 cells. PARG1 was also immunoprecipitated by rabbit anti-PARG1 antibody NBP1-05988.")
![Immunohistochemistry: Collagen II Antibody [NBP1-77795] - Staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.](http://images.novusbio.com/fullsize/Collagen-II-Antibody-Immunohistochemistry-NBP1-77795-img0005.jpg "Immunohistochemistry: Collagen II Antibody [NBP1-77795] - Staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100C - 20 minutes for antigen retrieval.")
![Immunohistochemistry: Collagen II Antibody [NBP1-77795] - Histological analysis and the expression of PIEZO1 (NBP1-78537) and collage II (NBP1-77795) in human nucleus pulposus tissues. A, B Hematoxylin and eosin (H&E) staining in human NP tissues of the control or the IVDD group (200×). C, D PIEZO1 immunostaining in human NP tissues of the control or the IVDD group (100×). E The percentage of PIEZO1 positive cells to all cells in the field (%). F, G Collagen II immunostaining in human NP tissues of the control or the IVDD group (100×). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35606793/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-77795_rabbit-polyclonal-collagen-ii-antibody-2552023153957.jpg)
![Western Blot: PHF6 Antibody [NB100-68262] - Detection of Human PHF6 on HeLa whole cell lysate.PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68261.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68262-img0004.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PHF6 Antibody [NB100-68262] - Detection of Human PHF6 on HeLa whole cell lysate.PHF6 was also immunoprecipitated by rabbit anti-PHF6 antibodies NB100-68260 and NB100-68261.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: PHF6 Antibody [NB100-68262] - Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti- PHF6 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PHF6-Antibody-Immunohistochemistry-NB100-68262-img0006.jpg)
![Western Blot: PHF6 Antibody [NB100-68262] - Image from a customer review testing on 293T cells.](http://images.novusbio.com/fullsize/PHF6-Antibody-Western-Blot-NB100-68262-img0003.jpg)
![Immunohistochemistry: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - IHC staining of Bouin Paraffin section ofhuman invasive breast tumor with107D2.08 (DAB brown)](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-107D2-08-Immunohistochemistry-DDX0300P-100-img0010.jpg "Immunohistochemistry: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - IHC staining of Bouin Paraffin section ofhuman invasive breast tumor with107D2.08 (DAB brown)")
![Flow Cytometry: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - Facs staining of CD11c-sorted tonsillar cells](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-107D2-08-Flow-Cytometry-DDX0300P-100-img0008.jpg)
![Immunohistochemistry-Paraffin: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - Paraffin embedded breast cancer tissue sections](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-107D2-08-Immunohistochemistry-Paraffin-DDX0300P-100-img0005.jpg)
![Immunohistochemistry-Paraffin: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100]](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-107D2-08-Immunohistochemistry-Paraffin-DDX0300P-100-img0006.jpg)
![Immunohistochemistry: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - IHC staining of frozen humantonsilsection with 106C2.02(Fast Blue)](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-107D2-08-Immunohistochemistry-DDX0300P-100-img0009.jpg)
![Flow Cytometry: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - Human PBMC enriched in monocytes and dendriticcells (Percollgradient) and analyzed by surface flowcytometry](http://images.novusbio.com/fullsize/IL-3-R-alpha-CD123-Antibody-107D2-08-Flow-Cytometry-DDX0300P-100-img0007.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0027.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine for 18 hours. PVDF (Polyvinylidene difluoride) membrane was probed with 0.5 ug/mL of Rabbit Anti-LC3B Polyclonal Antibody (Catalog # NB100-2220) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog# HAF008). A specific band was detected for LC3B at a molecular weight of approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Autophagy Antibody Pack [NB910-94877] - LC3B was detected in immersion fixed Cloroquine treated HeLa cells (left) but was not detected in LC3B knockout HeLa cells (right) using rabbit anti-human LC3B polyclonal antibody (Catalog #NB100-2220) at 0.3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0028.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - ERK phosphorylation in Atg5-/-cells depends on nutrient availability. Serum-deprived Atg5-/- MEFs display decreased ERK and p90RSK phosphorylation. Immunoblots for the indicated proteins in total lysates from 2 h serum-deprived WT and Atg5-/- MEFs. The bars represent mean+/-s.e.m. *P<0.05, ***P<0.001 compared with Con; Student's t-test, n=3. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms3799), licensed under a CC-BY license. ATG antibody (NB110-53818) used.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Knockout-Validated-NB910-94877-img0031.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Staining of ATG5 using NB110-53818 in human hepatocytes (liver) at 2.5ug/ml. 40X magnification.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0025.jpg)
![Simple Western: Autophagy Antibody Pack [NB910-94877] - Lane view shows a specific band for Beclin using NB110-87318 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Simple-Western-NB910-94877-img0037.jpg)
![Flow Cytometry: Autophagy Antibody Pack [NB910-94877] - An intracellular stain was performed on Hela Cells with NB110-56893 to detect ATG9 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG APC-conjugated Secondary Antibody (R&D Systems, F0111).image 5 of 5previousnextclose](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Flow-Cytometry-NB910-94877-img0035.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Analysis of a FFPE tissue section of human breast cancer using Beclin 1 antibody (NB110-87318) at 1:300 dilution, followed by a HRP labeled secondary antibody and DAB. Nuclei of the cells were counterstained with hematoxylin. The signal is strongest in a subset of stromal cells, likely the cancer associated fibroblasts.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0038.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - SCI leads to lysosomal dysfunction. Representative high magnification images ( x 60) of LC3 (NB100-2220, green) and CTSD (red) staining in VH of gray matter from sham and day 1 SCI animals. Decrease in number and intensity of CTSD puncta (lysosomes) and increase in LC3 puncta (autophagosomes) is apparent after SCI. Scale bar is 10 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/cddis2014527) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0029.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - Total protein from human HeLa and mouse Neuro2A cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Beclin-1 (NB110-87318) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0036.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0040.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody (NB110-60928) at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining of ATG16L1 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0041.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A protein using NB110-56893 in HEK293 lysates. Lane 1: siRNA ATG9A knockdown Lane 2: wildtype ATG9A.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0017.jpg)
![Western Blot: Autophagy Antibody Pack [NB910-94877] - CD133 promoted glucose uptake. E. Detection of autophagic genes in Huh-7con and Huh-7sh133 cells by Western blotting. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0056878) licensed under a CC-BY license. ATG9 detected using NB110-56893](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Western-Blot-NB910-94877-img0032.jpg)
![Immunohistochemistry: Autophagy Antibody Pack [NB910-94877] - Detection of ATG9A (NB110-56893) in mouse intestine.](http://images.novusbio.com/fullsize/Autophagy-Antibody-Pack-Immunohistochemistry-NB910-94877-img0018.jpg)