Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2625-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4184-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB2545U-100UG | |||
| Alexa Fluor 405 | FAB2545V-100UG | |||
| Alexa Fluor 488 | FAB2545G-100UG | |||
| Alexa Fluor 532 | FAB2545X-100UG | |||
| Alexa Fluor 594 | FAB2545T-100UG | |||
| Alexa Fluor 647 | FAB2545R-100UG | |||
| Alexa Fluor 700 | FAB2545N-100UG | |||
| Alexa Fluor 750 | FAB2545S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2545-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2218-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5420-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5169-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1407-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1108-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2496-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB35661-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34628-0.1mg | |||
![Immunocytochemistry/Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158] - 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/RPA2-Antibody-12F3-3-Immunocytochemistry-Immunofluorescence-NB100-158-img0003.jpg "<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: RPA2 Antibody (12F3.3) [NB100-158] - 2mM hydroxyurea treated (right) or untreated (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RPA32 protein stained by RPA32 antibody [12F3.3] Blue: Hoechst 33342 staining.")
![<b>Biological Strategies Validation. </b>Western Blot: RPA2 Antibody (12F3.3) [NB100-158] - Various whole cell extracts (30 ug) were separated by 12 % SDS-PAGE, and blotted with RPA32 antibody diluted by 1:500](http://images.novusbio.com/fullsize/RPA2-Antibody-12F3-3-Western-Blot-NB100-158-img0004.jpg)
![Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Western-Blot-NBP2-34243-img0006.jpg "Western Blot: EGFR Antibody (H9B4) [NBP2-34243] - Western Blot Analysis of A431 cell lysate using EGFR Mouse Monoclonal Antibody (H9B4).")
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of A431 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0001.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Simple Western lane view shows a specific band for EGF R/ErbB1 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0002.jpg)
![Simple Western: EGFR Antibody (H9B4) [NBP2-34243] - Electropherogram image of the corresponding Simple Western lane. EGF R/ErbB1 antibody was used at 10 ug/ml dilution of A431 and HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Simple-Western-NBP2-34243-img0004.jpg)
![Protein Array: EGFR Antibody (H9B4) [NBP2-34243] - Analysis of Protein Array containing more than 19,000 full-length human proteins using EGFR Antibody (H9B4) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/EGFR-Antibody-H9B4-Protein-Array-NBP2-34243-img0005.jpg)