BoNT-B Light Chain Antibody [Unconjugated]

• Reactivity: Ba-Cb
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

BoNT-B Light Chain Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant Clostridium BoNT-B Light Chain
  Pro2-His428
  Accession # P10844
• Specificity: Detects C. botulinum BoNT-B Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant Light Chains of BoNT-A, -E, or recombinant Heavy Chains of BoNT-C, or -D is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: boNT/B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Reactivity Notes

C. botulinum

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BoNT-B Light Chain Antibody [Unconjugated]

• BoNTB Light Chain
• BoNT-B Light Chain
• DcD

Background

Botulinum Neurotoxin Type B is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4). E. coli expressed recombinant light chains are active proteases. In the absence of the heavy chains, however, they lack toxicity because they cannot enter into host cells.

1. Campbell K.D. et al. (1993) J. Clin. Microbiol. 31:2255.
2. Montecucco, C. and Giampietro, S. (1993) Trends Biochem. Sci. 18:324.
3. Turton, K. et al. (2002) Trends Biochem. Sci. 27:552.
4. Schiavo, G. et al. (2000) Physiol. Rev. 80:717.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BoNT-B Light Chain Antibody (AF5420)(3)

Publications using AF5420

• L Pan, A Trimarco, AJ Zhang, K Fujimori, Y Urade, LO Sun, C Taveggia, Y Zhang Oligodendrocyte-lineage cell exocytosis and L-type prostaglandin D synthase promote oligodendrocyte development and myelination Elife, 2023-02-13;12(0):. 2/13/2023 [PMID: 36779701]
• M Golan, J Boulanger-, A Pinot, P Fontanaud, A Faucherre, DS Gajbhiye, L Hollander-, T Fiordelisi, AO Martin, P Mollard Synaptic communication mediates the assembly of a self-organizing circuit that controls reproduction Science Advances, 2021-02-19;7(8):. 2021-02-19 [PMID: 33608269] (IHC, Zebrafish)
• China Y. Lim, Jennifer H. Granger, Marc D. Porter SERS detection of Clostridium botulinum neurotoxin serotypes A and B in buffer and serum: Towards the development of a biodefense test platform Analytica Chimica Acta: X 2019-03-01 [PMID: 33186413]

Bioinformatics

• Gene Symbol: boNT/B
• Uniprot:
  • P10844(C. botulinum)