Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP2-44230AFP488 | |||
| Alexa Fluor Plus 555 | NBP2-44230AFP555 | |||
| Alexa Fluor Plus 594 | NBP2-44230AFP594 | |||
| Alexa Fluor Plus 647 | NBP2-44230AFP647 | |||
| Alexa Fluor Plus 680 | NBP2-44230AFP680 | |||
| Biotin | NB600-614-0.1mg | |||
| HRP | NB120-20732-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-181SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2187-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0440A488-100 | |||
| Alexa Fluor 546 | DDX0440A546-100 | |||
| Alexa Fluor 647 | DDX0440A647-100 | |||
| Biotin | DDX0440B-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5985-SP | |||
![Western Blot: Pepsin Antibody [NBP2-44230] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Image using the HRP form of this antibody.](http://images.novusbio.com/fullsize/Pepsin-Antibody-Western-Blot-NBP2-44230-img0005.jpg "Western Blot: Pepsin Antibody [NBP2-44230] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Image using the HRP form of this antibody.")
![Immunohistochemistry-Paraffin: Pepsin Antibody [NBP2-44230] - Staining: Gastric mucosa (containing fundic gland with zymogenic cells) homogeneus positivity in the secretory granules of glandular chief cells and in secretory granular of neck cells (Shown Left and Center) negative control with normal rabbit IgG (Shown right).](http://images.novusbio.com/fullsize/Pepsin-Antibody-Immunohistochemistry-Paraffin-NBP2-44230-img0001.jpg)
![Western Blot: Pepsin Antibody [NBP2-44230] - lane 1: Pepsin. Lane 2: None. Load: 50 ng per lane.](http://images.novusbio.com/fullsize/Pepsin-Antibody-Western-Blot-NBP2-44230-img0002.jpg)
![Western Blot: Pepsin Antibody [NBP2-44230] - Western Blot: Pepsin Antibody [Biotin] [NB600-614] - Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 41 kDa, 41 kDa for Pepsin. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Pepsin-Antibody-Western-Blot-NBP2-44230-img0003.jpg)
![Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.](http://images.novusbio.com/fullsize/DMC1-Antibody-1D12-4-Western-Blot-NB100-181-img0003.jpg "Western Blot: DMC1 Antibody (1D12/4) [NB100-181] - Analysis of DMC1 on human testis using NB100-181.")
![Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.](http://images.novusbio.com/fullsize/HGF-R-c-MET-Antibody-556-Western-Blot-NBP2-43641-img0001.jpg "Western Blot: HGFR/c-MET Antibody (556) [NBP2-43641] - Analysis of various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with MET antibody diluted by 1:1000.")
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-26112025182908.jpg)
![<b>Genetic Strategies Validation. </b>Wild-type (WT) and c-Met knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251843456.jpg)
![<b>Genetic Strategies Validation. </b>Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with c-Met antibody [GT556] (NBP2-43641) diluted at 1:500.](http://images.novusbio.com/fullsize/nbp2-43641_mouse-monoclonal-hgfr-c-met-antibody-556-western-blot-261120251850424.jpg)
![Western Blot: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - IP of I^125-labeled mono-DC with Neuropilin-1 antibody (211H6.01) [DDX0440P-100]. Theoretical molecular weight 103 kDa. Observed molecular weight ~100 kDa.](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-211H6-01-Western-Blot-DDX0440P-100-img0005.jpg "Western Blot: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - IP of I^125-labeled mono-DC with Neuropilin-1 antibody (211H6.01) [DDX0440P-100]. Theoretical molecular weight 103 kDa. Observed molecular weight ~100 kDa.")
![Immunohistochemistry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - IHC staining of human tonsil using Neuropilin-1 antibody (211H6.01) [DDX0440P-100].](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-211H6-01-Immunohistochemistry-DDX0440P-100-img0002.jpg)
![Flow Cytometry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - FACS analysis of human PBMC using Neuropilin-1 antibody (211H6.01) [DDX0440P-100].](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-211H6-01-Flow-Cytometry-DDX0440P-100-img0003.jpg)
![Flow Cytometry: Neuropilin-1 Antibody (211H6.01) [DDX0440P-100] - FACS analysis of human monocyte-derived DC using Neuropilin-1 antibody (211H6.01) [DDX0440P-100].](http://images.novusbio.com/fullsize/Neuropilin-1-Antibody-211H6-01-Flow-Cytometry-DDX0440P-100-img0001.jpg)
![Western Blot: CAD Antibody [NB100-61614] - Whole cell lysate (50 ug) from HEK293T, K-562, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody NB100-61614 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CAD-Antibody-Western-Blot-NB100-61614-img0009.jpg "Western Blot: CAD Antibody [NB100-61614] - Whole cell lysate (50 ug) from HEK293T, K-562, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody NB100-61614 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry-Paraffin: CAD Antibody [NB100-61614] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CAD used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-NB100-61614-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CAD Antibody [NB100-61614] - Detection of human CAD by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAD antibody NB100-61614 (lot NB100-61614-3) used for IP at 6 ug per reaction. CAD was also immunoprecipitated by a previous lot of this antibody (lot NB100-61614-2) and another rabbit anti-CAD antibody. For blotting immunoprecipitated CAD, NB100-61614 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CAD-Antibody-Immunoprecipitation-NB100-61614-img0010.jpg)
![Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - Detection of mouse hPrp3p by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-hPrp3p (NBP1-19114) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PRPF3-Antibody-Immunohistochemistry-Paraffin-NBP1-19114-img0008.jpg "Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - Detection of mouse hPrp3p by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-hPrp3p (NBP1-19114) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB")
![Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-hPrp3p used at a dilution of 1:100.](http://images.novusbio.com/fullsize/PRPF3-Antibody-Immunohistochemistry-Paraffin-NBP1-19114-img0001.jpg)
![Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - FFPE section of mouse teratoma. Affinity purified rabbit anti-hPrp3p used at a dilution of 1:100.](http://images.novusbio.com/fullsize/PRPF3-Antibody-Immunohistochemistry-Paraffin-NBP1-19114-img0002.jpg)
![Immunohistochemistry: PRPF3 Antibody [NBP1-19114] - Sample: FFPE section of human breast carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-hPrp3p used at a dilution of 1:5,000 (0.2ug/ml) and 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PRPF3-Antibody-Immunohistochemistry-NBP1-19114-img0006.jpg)
![Immunohistochemistry-Paraffin: PRPF3 Antibody [NBP1-19114] - Detection of human hPrp3p by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-hPrp3p (NBP1-19114) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PRPF3-Antibody-Immunohistochemistry-Paraffin-NBP1-19114-img0007.jpg)
![Immunoprecipitation: PRPF3 Antibody [NBP1-19114] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NBP1-19114 used for IP at 3 mcg/mg lysate. hPrp3p was also immunoprecipitated by NBP1-19115 which recognizes a downstream epitope](http://images.novusbio.com/fullsize/PRPF3-Antibody-Immunoprecipitation-NBP1-19114-img0005.jpg)
![Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.](http://images.novusbio.com/fullsize/MBD2-Antibody-Western-Blot-NB100-81657-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: MBD2 Antibody [NB100-81657] - Detection of Human and Mouse MBD2 on HeLa whole cell lysate using NB100-81657. MBD2 was also immunoprecipitated by rabbit anti-MBD2 antibody NB100-81656.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: MBD2 Antibody [NB100-81657] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-MBD2 used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/MBD2-Antibody-Immunohistochemistry-NB100-81657-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: STK4 Antibody [NBP1-31233] - Paraformaldehyde-fixed U2OS, using STK4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/STK4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31233-img0004.jpg "Immunocytochemistry/Immunofluorescence: STK4 Antibody [NBP1-31233] - Paraformaldehyde-fixed U2OS, using STK4 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2000.")
![Immunohistochemistry-Paraffin: STK4 Antibody [NBP1-31233] - Human lung adenocarcinoma. STK4 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/STK4-Antibody-Immunohistochemistry-Paraffin-NBP1-31233-img0008.jpg)
![Immunohistochemistry-Paraffin: STK4 Antibody [NBP1-31233] - Cal27 xenograft, using MST1 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/STK4-Antibody-Immunohistochemistry-Paraffin-NBP1-31233-img0005.jpg)
![Immunoprecipitation: STK4 Antibody [NBP1-31233] - HepG2 whole cell extract A. 50 ug HepG2 whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of STK4 protein by 4 ug STK4 antibody 7.5 % SDS-PAGE The immunoprecipitated STK4 protein was detected by STK4 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/STK4-Antibody-Immunoprecipitation-NBP1-31233-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: MST1/STK4 Antibody [NBP1-31233] - Wild-type (WT) and MST1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MST1 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31233_rabbit-polyclonal-stk4-antibody-1610202419405747.jpg)
![Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Western-Blot-NBP3-15230-img0005.jpg "Western Blot: PKM2 Antibody (7B2) [NBP3-15230] - Positive WB detected in: SH-SY5Y whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, Raji whole cell lysate, 293 whole cell lysate, Mouse brain tissue. All lanes: PKM2 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 58, 59, 57 KDa. Observed band size: 58 KDa.")
![Immunocytochemistry/Immunofluorescence: PKM2 Antibody (7B2) [NBP3-15230] - Immunofluorescence staining of Hela Cells with at PKM2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunocytochemistry-Immunofluorescence-NBP3-15230-img0002.jpg)
![Immunohistochemistry: PKM2 Antibody (7B2) [NBP3-15230] - IHC image of PKM2 antibody diluted at 1:100 and staining in paraffin-embedded human lung cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunohistochemistry-NBP3-15230-img0004.jpg)
![Flow Cytometry: PKM2 Antibody (7B2) [NBP3-15230] - Overlay histogram showing HepG2 cells stained with PKM2 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Flow-Cytometry-NBP3-15230-img0001.jpg)
![Immunoprecipitation: PKM2 Antibody (7B2) [NBP3-15230] - Immunoprecipitating PKM2 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of PKM2 antibody in Hela whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: PKM2 antibody (2ug)+ Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (10ug).](http://images.novusbio.com/fullsize/PKM2-Antibody-7B2-Immunoprecipitation-NBP3-15230-img0003.jpg)
![Western Blot: Casein Kinase 1 delta Antibody [NBP1-21376] - Whole cell lysate from HeLa cells. CKI delta was also IPed by another antibody directed to an epitope that is common to CKI epsilon and delta. CKI epsilon was IPed and blotted using NBP1-21375.](http://images.novusbio.com/fullsize/Casein-Kinase-1-delta-Antibody-Western-Blot-NBP1-21376-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Casein Kinase 1 delta Antibody [NBP1-21376] - Whole cell lysate from HeLa cells. CKI delta was also IPed by another antibody directed to an epitope that is common to CKI epsilon and delta. CKI epsilon was IPed and blotted using NBP1-21375.")