Pepsin Antibody [Biotin]

Western Blot: Pepsin Antibody [Biotin] [NB600-614] - Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody Biotin Conjugated 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 41 kDa, 41 kDa for Pepsin.

Western Blot of Goat anti-Pepsin Antibody Biotin Conjugated. Lane 1: Pepsin. Load: 50 ng per lane. Primary antibody: Goat anti-Pepsin Antibody [Biotin]1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin

• Reactivity: Po
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Pepsin Antibody [Biotin] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]
• Immunogen: Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach. (Uniprot: P00791)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:2000
• Application Notes: This product has been tested by ELISA and western. This product is suitable for Immunohistochemistry. Expect band at 41.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Reactivity Notes

Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Pepsin Antibody [Biotin]

• DKFZp666J2410
• ec 3.4.23.1
• Pepsin a precursor
• Pepsinogen 1 second locus
• Pepsinogen 3 group 1
• Pepsinogen 4 group I
• Pepsinogen 5 group I
• Pepsinogen A
• Pepsinogen a3
• Pepsinogen A4
• Pepsinogen A5
• Pepsinogen group 1
• Pepsinogen I
• Pga 3
• Pga 4
• Pga 5
• pga3
• pga4
• pga5

Background

Digestive enzyme with particularly broad specificity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P00791(Porcine)