Pepsin Antibody [HRP]

Western Blot: Pepsin Antibody [HRP] [NB120-20732] - Lane 1: Pepsin. Lane 2: none. Load: 100 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for30 min at RT. Predicted/Observed size: 35 kDa for Pepsin.

Western Blot of Peroxidase conjugated Goat anti-Pepsin antibody. Lane 1: Pepsin. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Peroxidase goat pepsin antibody at 1:1,000 for 60 min at RT Block for 30 min at RT. Predicted/Observed size: 35 kDa for Pepsin. Other band(s): none.

• Reactivity: Po
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: HRP
• Concentration: LYOPH

Pepsin Antibody [HRP] Summary

• Description: Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
  
  Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin [Porcine Stomach]
• Immunogen: Pepsin Antibody was produced by repeated immunizations with pork Pepsin protein isolated from swine stomach. (Uniprot: P00791)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:10000
  • Immunohistochemistry 1:250-1:1250
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:500-1:2500
• Application Notes: This product has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Pepsin Antibody [HRP]

• DKFZp666J2410
• ec 3.4.23.1
• Pepsin a precursor
• Pepsinogen 1 second locus
• Pepsinogen 3 group 1
• Pepsinogen 4 group I
• Pepsinogen 5 group I
• Pepsinogen A
• Pepsinogen a3
• Pepsinogen A4
• Pepsinogen A5
• Pepsinogen group 1
• Pepsinogen I
• Pga 3
• Pga 4
• Pga 5
• pga3
• pga4
• pga5

Background

Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P00791(Porcine)