Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6174-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2340-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1244-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2504-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92685SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB929P | |||
| Carrier Free | MAB929-SP | |||
| Carrier Free | FAB929S-100UG | |||
| Carrier Free | FAB929R-100UG | |||
| Carrier Free | FAB929N-100UG | |||
| Carrier Free | FAB929G-100UG | |||
| Carrier Free | FAB929V-100UG | |||
| Carrier Free | FAB929U-100UG | |||
| Carrier Free | FAB929T-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1196-SP | |||
| Carrier Free | MAB1196-500 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB2624U-100UG | |||
| Alexa Fluor 405 | FAB2624V-100UG | |||
| Alexa Fluor 488 | FAB2624G-100UG | |||
| Alexa Fluor 532 | FAB2624X-100UG | |||
| Alexa Fluor 594 | FAB2624T-100UG | |||
| Alexa Fluor 647 | FAB2624R-100UG | |||
| Alexa Fluor 700 | FAB2624N-100UG | |||
| Alexa Fluor 750 | FAB2624S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2624-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2337-SP | |||
![Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human prostate carcinoma. Affinity purified rabbit anti-Emsy used at a dilution of 1:100 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0015.jpg)
![Western Blot: EMSY Antibody [NB100-545] - Nuclear extract (NE) from HeLa cells.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0010.jpg)
![Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: EMSY Antibody [NB100-545] - Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 (lot NB100-545-2) used for IP at 6 ug/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot NB100-545-1) and another rabbit anti-EMSY antibody. For blotting immunoprecipitated EMSY, NB100-545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunoprecipitation-NB100-545-img0014.jpg)
![Western Blot: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, NBP1-92685, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction.Double band at 56-68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.](http://images.novusbio.com/fullsize/TAF15-Antibody-4D71-Western-Blot-NBP1-92685-img0002.jpg "Western Blot: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of cytosol or nuclear enriched fractions of cell line lysates using mouse mAb to TAF15, NBP1-92685, dilution 1:1,000: [1] protein standard, [2] NIH-3T3 cytosol, [3] NIH-3T3 nuclear fraction, [4] HeLa cytosol, and [5] HeLa nuclear fraction.Double band at 56-68kDa detected predominantly in the nuclear enriched fraction corresponds to the TAF15 protein.")
![Immunocytochemistry/Immunofluorescence: TAF15 Antibody (4D71) [NBP1-92685] - Analysis of HeLa cells stained with mouse mAb to TATA-box binding protein associated factor 15, TAF15, NBP1-92685, dilution 1:500 in red, and costained with rabbit pAb to GAPDH, dilution 1:1,000 in green. Blue is Hoechst staining of nuclear DNA. The NBP1-92685 antibody reveals TAF15 protein expressed exclusively in the nuclei of the cells, while GAPDH antibody produces strong cytoplasmic staining.](http://images.novusbio.com/fullsize/TAF15-Antibody-4D71-Immunocytochemistry-Immunofluorescence-NBP1-92685-img0001.jpg)
![Western Blot: TIP30 Antibody [NBP2-20646] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 12% SDS PAGE gel, diluted at 1:5000.](http://images.novusbio.com/fullsize/TIP30-Antibody-Western-Blot-NBP2-20646-img0001.jpg "Western Blot: TIP30 Antibody [NBP2-20646] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa 12% SDS PAGE gel, diluted at 1:5000.")
![Immunocytochemistry/Immunofluorescence: TIP30 Antibody [NBP2-20646] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TIP30 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/TIP30-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20646-img0003.jpg)
![Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646] - Paraffin-embedded gastric cancer. TIP30 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/TIP30-Antibody-Immunohistochemistry-Paraffin-NBP2-20646-img0007.jpg)
![Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646] - Immunohistochemical analysis of paraffin-embedded Breast CA, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/TIP30-Antibody-Immunohistochemistry-Paraffin-NBP2-20646-img0002.jpg)
![Immunohistochemistry-Paraffin: TIP30 Antibody [NBP2-20646] - Paraffin-embedded mouse muscle. TIP30 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/TIP30-Antibody-Immunohistochemistry-Paraffin-NBP2-20646-img0006.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-20058] - Human ovarian carcinoma. Rad51 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Rad51-Antibody-Immunohistochemistry-Paraffin-NBP2-20058-img0005.jpg "Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-20058] - Human ovarian carcinoma. Rad51 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min")
![Western Blot: Rad51 Antibody [NBP2-20058] - Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Rad51-Antibody-Western-Blot-NBP2-20058-img0001.jpg)
![Immunohistochemistry-Paraffin: Rad51 Antibody [NBP2-20058] - Human breast carcinoma. Rad51 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/Rad51-Antibody-Immunohistochemistry-Paraffin-NBP2-20058-img0004.jpg)
![Rad51 antibody immunoprecipitates Rad51 protein in IP experiments.IP samples: Jurkat whole cell extractA. 40 ug Jurkat whole cell extractB. Control with 4 ug of preimmune Rabbit IgGC. Immunoprecipitation of Rad51 protein by 4 ug Rad51 antibody (NBP2-20058)5 % SDS-PAGEThe immunoprecipitated Rad51 protein was detected by Rad51 antibody (NBP2-20058) diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-20058_rabbit-polyclonal-rad51-antibody-immunoprecipitation-261120251843455.jpg)
