Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB29071-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2627-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6018-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1244-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50355-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-23483SS | |||
| Azide and BSA Free | NBP2-80590 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3305-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB2176U-100UG | |||
| Alexa Fluor 405 | FAB2176V-100UG | |||
| Alexa Fluor 488 | FAB2176G-100UG | |||
| Alexa Fluor 532 | FAB2176X-100UG | |||
| Alexa Fluor 594 | FAB2176T-100UG | |||
| Alexa Fluor 647 | FAB2176R-100UG | |||
| Alexa Fluor 700 | FAB2176N-100UG | |||
| Alexa Fluor 750 | FAB2176S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2176-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB929P | |||
| Carrier Free | MAB929-SP | |||
| Carrier Free | FAB929S-100UG | |||
| Carrier Free | FAB929R-100UG | |||
| Carrier Free | FAB929N-100UG | |||
| Carrier Free | FAB929G-100UG | |||
| Carrier Free | FAB929V-100UG | |||
| Carrier Free | FAB929U-100UG | |||
| Carrier Free | FAB929T-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2485-SP | |||
![Flow Cytometry: TRBC1 Antibody (JOVI.1) - BSA Free [NBP2-50355] - A surface stain was performed on Jurkat cells NBP2-50355AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/TCR-beta-Antibody-JOVI-1-Flow-Cytometry-NBP2-50355-img0001.jpg "Flow Cytometry: TRBC1 Antibody (JOVI.1) - BSA Free [NBP2-50355] - A surface stain was performed on Jurkat cells NBP2-50355AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.")
![Western Blot: V5 Epitope Tag Antibody [NB600-379] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).](http://images.novusbio.com/fullsize/V5-Epitope-Tag-Antibody-Western-Blot-NB600-379-img0001.jpg "Western Blot: V5 Epitope Tag Antibody [NB600-379] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.2 ug/ml (1:5,000).")
![Western Blot: Bat Immunoglobulin Antibody (BT1-4F10) [NBP2-23483] - Analysis of whole serum as detected by NBP2-23483. Left side shows the non-reduced lane, mainly IgG because all the chains are attached to each other. The right side is the reduced lane, where the different chains are separated and the one directly bound by the mAb (Light chain) is visualized.](http://images.novusbio.com/fullsize/Bat-Immunoglobulin-Antibody-BT1-4F10-Western-Blot-NBP2-23483-img0001.jpg "Western Blot: Bat Immunoglobulin Antibody (BT1-4F10) [NBP2-23483] - Analysis of whole serum as detected by NBP2-23483. Left side shows the non-reduced lane, mainly IgG because all the chains are attached to each other. The right side is the reduced lane, where the different chains are separated and the one directly bound by the mAb (Light chain) is visualized.")
![Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human prostate carcinoma. Affinity purified rabbit anti-Emsy used at a dilution of 1:100 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0015.jpg)
![Western Blot: EMSY Antibody [NB100-545] - Nuclear extract (NE) from HeLa cells.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0010.jpg)
![Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: EMSY Antibody [NB100-545] - Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 (lot NB100-545-2) used for IP at 6 ug/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot NB100-545-1) and another rabbit anti-EMSY antibody. For blotting immunoprecipitated EMSY, NB100-545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunoprecipitation-NB100-545-img0014.jpg)
