wnvNS3 Protease Antibody (345712) [Unconjugated]

Western blot shows lysates of Recombinant Viral wnvNS3 Protease. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Viral wnvNS3 Protease Monoclonal Antibody (Catalog # MAB29071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for full length wnvNS3 Protease at approximately 32 kDa and C-terminal NS3 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB, IP, B/N
• Clone: 345712
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

wnvNS3 Protease Antibody (345712) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant wnvNS3 Protease protein
  Ser1423-Lys1470, Gly1506-Leu1689
  Accession # ABD85079
• Specificity: Detects West Nile NS3 Protease in Western blots.
• Source: N/A
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLY
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Neutralization 15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in TBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile Tris-buffered saline, pH 7.3.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for wnvNS3 Protease Antibody (345712) [Unconjugated]

• wnvNS3 Protease

Background

Infection of mosquito-borne West Nile Virus can cause severe neurological disease and can be epidemic. Two non-structural proteins, NS3 and NS2b, play an essential role in viral replication and are therefore a potential target for treatment and prevention of West Nile Virus disease. NS3 consists of a trypsin-like serine protease with a catalytic triad (His51, Asp75, Ser135) and a putative helicase. Requiring NS2b as the cofactor, NS3 protease processes viral polyprotein precursor (1, 2). The purified recombinant protein used as an immunogen consists of three forms: the full-length fusion protein, the N-terminal NS2b, and the C-terminal NS3 with a G₄SG₄ linker. NS3 protease has a relatively narrow substrate specificity that prefers Arg in P1 and Lys in P2.

1. Nall, T.A. et al. (2004) J. Biol. Chem. 279:48535.
2. Chappell, K.J. et al. (2005) J. Biol. Chem. 274:2896.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for wnvNS3 Protease Antibody (MAB29071)(3)

Publications using MAB29071

• Harendra S. Chahar, Shuiping Chen, N. Manjunath P-body components LSM1, GW182, DDX3, DDX6 and XRN1 are recruited to WNV replication sites and positively regulate viral replication Virology 2013-02-05 [PMID: 23102969]
• Xu Z, Waeckerlin R, Urbanowski MD West Nile virus infection causes endocytosis of a specific subset of tight junction membrane proteins. PLoS ONE, 2012-05-24;7(5):e37886. 2012-05-24 [PMID: 22655077] (ICC, Mouse)
• Xu Z, Anderson R, Hobman TC The capsid-binding nucleolar helicase DDX56 is important for infectivity of West Nile virus. J. Virol., 2011-03-16;85(11):5571-80. 2011-03-16 [PMID: 21411523] (ICC, Human)

Bioinformatics

• Gene Symbol: POLY
• Uniprot:
  • ABD85079(Viral)