Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2449-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1350-SP | |||
| Carrier Free | FAB1350V-100UG | |||
| Carrier Free | FAB1350U-100UG | |||
| Carrier Free | FAB1350T-100UG | |||
| Carrier Free | FAB1350S-100UG | |||
| Carrier Free | FAB1350R-100UG | |||
| Carrier Free | FAB1350N-100UG | |||
| Carrier Free | FAB1350G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB6647F | |||
| Carrier Free | FAB6647P | |||
| Carrier Free | AF691-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1013-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1130-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NBP1-00641AF405 | |||
| Alexa Fluor 488 | NBP1-00641AF488 | |||
| Alexa Fluor 647 | NBP1-00641AF647 | |||
| Alexa Fluor 700 | NBP1-00641AF700 | |||
| Biotin | NBP1-00641B | |||
| DyLight 405 | NBP1-00641V | |||
| DyLight 488 | NBP1-00641G | |||
| DyLight 550 | NBP1-00641R | |||
| DyLight 650 | NBP1-00641C | |||
| FITC | NBP1-00641F | |||
| HRP | NBP1-00641H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-00641SS | |||
![Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0017.jpg "<b>Biological Strategies Validation. </b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Detection of Human PKD2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PKD2 antibody NB100-636 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PKD2 antibody (lot For blotting immunoprecipitated PKD2, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Western-Blot-NB100-636-img0015.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Protein Kinase D2 Antibody [NB100-636] - Samples: Whole cell lysate (A. 30 ug/lane; B. 200 mcg/IP) from HEK293 cells that were untreated or transfected with expression constructs for PKD1 and PKD3 as controls for reactivity with these homologs. Antibodies: A. Affinity purified rabbit anti- NB100-636 used at the indicated oncentrations for WB. B. Affinity purified rabbit anti- NB100-636 and NB100-2299 used at 2 ug/200 mcg lysate for IP. Immunoprecipitated PKD2 was detected by WB using anti-PKD2 antibody NB100-2299 at 1 ug/ml. Detection: Chemiluminescence with a 10 second exposure.](http://images.novusbio.com/fullsize/Protein-Kinase-D2-Antibody-Immunoprecipitation-NB100-636-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ECHS1 protein stained by ECHS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16263-img0012.jpg "Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ECHS1 protein stained by ECHS1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.")
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse ovary by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16263-img0009.jpg)
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16263-img0005.jpg)
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16263-img0007.jpg)
![Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP2-16263] - ECHS1 antibody detects ECHS1 protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse ovary. ECHS1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECHS1-Antibody-Immunohistochemistry-Paraffin-NBP2-16263-img0008.jpg)
![Western Blot: ECHS1 Antibody [NBP2-16263] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with ECHS1 antibody [N1C2] (NBP2-16263) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16263_rabbit-polyclonal-echs1-antibody-91020242041210.jpg)
![ECHS1 antibody [N1C2] detects ECHS1 protein at mitochondria by immunofluorescent analysis.Sample: A431 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min.Green: ECHS1 protein stained by ECHS1 antibody [N1C2] (NBP2-16263) diluted at 1:200.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16263_rabbit-polyclonal-echs1-antibody-immunocytochemistry-immunofluorescence-261120251837509.jpg)
![Western Blot: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/XPG-Antibody-Western-Blot-NB100-74611-img0010.jpg "Western Blot: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: XPG Antibody [NB100-74611] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ERCC5/XPG used at a dilution of 1:200 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/XPG-Antibody-Immunohistochemistry-Paraffin-NB100-74611-img0011.jpg)
![Immunoprecipitation: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for IP at 6 ug per reaction. For blotting immunoprecipitated ERCC5/XPG, NB100-74611 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/XPG-Antibody-Immunoprecipitation-NB100-74611-img0009.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr-p84-Thoc1 Antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0011.jpg "Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with hHpr-p84-Thoc1 Antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunohistochemistry-Paraffin-NBP2-16822-img0002.jpg)
![Western Blot: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Western-Blot-NBP2-16822-img0004.jpg)
![Immunoprecipitation: hHpr1-p84-Thoc1 Antibody [NBP2-16822] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of p84 protein expressing HepG2 cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of p84 by 3 ug of Nuclear Matrix Protein p84 antibody [C1C3] 7.5% SDS-PAGE The immunoprecipitated p84 protein was detected by Nuclear Matrix Protein p84 antibody [C1C3] diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.](http://images.novusbio.com/fullsize/hHpr1-p84-Thoc1-Antibody-Immunoprecipitation-NBP2-16822-img0006.jpg)
![Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Nuclear Matrix Protein p84 antibody [C1C3] (NBP2-16822) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16822_rabbit-polyclonal-hhpr1-p84-thoc1-antibody-western-blot-2611202518274610.jpg)
![Nuclear Matrix Protein p84 antibody [C1C3] detects Nuclear Matrix Protein p84 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Nuclear Matrix Protein p84 protein stained by Nuclear Matrix Protein p84 antibody [C1C3] (NBP2-16822) diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-16822_rabbit-polyclonal-hhpr1-p84-thoc1-antibody-immunocytochemistry-immunofluorescence-261120251848236.jpg)
![Western Blot: PDE9A Antibody [NBP1-00641] - WB analysis of PDE9A in HeLa whole cell lysate.](http://images.novusbio.com/fullsize/PDE9A-Antibody-Western-Blot-NBP1-00641-img0002.jpg "Western Blot: PDE9A Antibody [NBP1-00641] - WB analysis of PDE9A in HeLa whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: PDE9A Antibody [NBP1-00641] - PDE9A antibody was tested in NIH/3T3 cells with Dylight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PDE9A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00641-img0004.jpg)
![Immunohistochemistry: PDE9A Antibody [NBP1-00641] - PDE9A antibody was tested in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PDE9A-Antibody-Immunohistochemistry-NBP1-00641-img0006.jpg)
![Western Blot: PARP10 Antibody [NB100-2157] - Detection of Human PARP10 on HeLa whole cell lysate using NB100-2157. PARP10 was also immunoprecipitated using other rabbit anti-PARP10 antibodies.](http://images.novusbio.com/fullsize/PARP10-Antibody-Western-Blot-NB100-2157-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PARP10 Antibody [NB100-2157] - Detection of Human PARP10 on HeLa whole cell lysate using NB100-2157. PARP10 was also immunoprecipitated using other rabbit anti-PARP10 antibodies.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PARP10 Antibody [NB100-2157] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PARP10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/PARP10-Antibody-Immunohistochemistry-Paraffin-NB100-2157-img0009.jpg)
