Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76973-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77067SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77326-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52666-0.025mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-22358 | |||
| DyLight 350 | NB7237UV | |||
| DyLight 405 | NB7237V | |||
| DyLight 488 | NB7237G | |||
| DyLight 550 | NB7237R | |||
| DyLight 594 | NB7237DL594 | |||
| DyLight 650 | NB7237C | |||
| DyLight 680 | NB7237FR | |||
| DyLight 755 | NB7237IR | |||
| FITC | NB7237F | |||
| HRP | NB7238 | |||
| Janelia Fluor 525 | NB7237JF525 | |||
| Janelia Fluor 669 | NB7237JF669 | |||
| PE | NB7237PE | |||
| PerCP | NB7237PCP | |||
![Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - G3BP monoclonal antibody (M01), clone 2F3 Analysis of G3BP expression in A-431.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Western-Blot-H00010146-M01-img0013.jpg "Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - G3BP monoclonal antibody (M01), clone 2F3 Analysis of G3BP expression in A-431.")
![Immunocytochemistry/Immunofluorescence: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Immunocytochemistry-Immunofluorescence-H00010146-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Immunohistochemistry-Paraffin-H00010146-M01-img0010.jpg)
![Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi ( Cat # H00010146-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody (M01), clone 2F3 (Cat # H00010146-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Western-Blot-H00010146-M01-img0011.jpg)
![Western Blot: G3BP1 Antibody (2F3) [H00010146-M01] - Analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody (M01), clone 2F3.Lane 1: G3BP transfected lysate(52.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-Western-Blot-H00010146-M01-img0014.jpg)
![ELISA: G3BP1 Antibody (2F3) [H00010146-M01] - Detection limit for recombinant GST tagged G3BP1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/G3BP1-Antibody-2F3-ELISA-H00010146-M01-img0015.jpg)
![Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - INPPL1 monoclonal antibody (M01), clone 3E6 Analysis of INPPL1 expression in HeLa.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-3E6-Western-Blot-H00003636-M01-img0004.jpg "Western Blot: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - INPPL1 monoclonal antibody (M01), clone 3E6 Analysis of INPPL1 expression in HeLa.")
![ELISA: SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - SHIP2/INPPL1 Antibody (3E6) [H00003636-M01] - Detection limit for recombinant GST tagged INPPL1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-3E6-ELISA-H00003636-M01-img0005.jpg)
![Immunohistochemistry-Paraffin: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01] - Immunohistochemistry of iron handling proteins in healthy kidney. Representatives images of ZIP8 (a), ZIP14 (b), divalent metal transporter 1 (DMT1; c), L-ferritin (d), H-ferritin (e), and ferroportin (f) staining in healthy kidney. Renal structures indicated as glomerulus (g), proximal tubule (PT), distal tubule (DT). Scale bar 20uM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-27107-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NRAMP2-SLC11A2-DMT1-Antibody-4C6-Immunohistochemistry-Paraffin-H00004891-M01-img0009.jpg "Immunohistochemistry-Paraffin: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01] - Immunohistochemistry of iron handling proteins in healthy kidney. Representatives images of ZIP8 (a), ZIP14 (b), divalent metal transporter 1 (DMT1; c), L-ferritin (d), H-ferritin (e), and ferroportin (f) staining in healthy kidney. Renal structures indicated as glomerulus (g), proximal tubule (PT), distal tubule (DT). Scale bar 20uM. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-018-27107-8) licensed under a CC-BY license.")
![Immunohistochemistry-Paraffin: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01] - Analysis of monoclonal antibody to SLC11A2 on formalin-fixed paraffin-embedded human endometrium cancer. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/NRAMP2-SLC11A2-DMT1-Antibody-4C6-Immunohistochemistry-Paraffin-H00004891-M01-img0006.jpg)
![ELISA: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01] - Detection limit for recombinant GST tagged SLC11A2 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/NRAMP2-SLC11A2-DMT1-Antibody-4C6-ELISA-H00004891-M01-img0008.jpg)
![Immunohistochemistry: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01] - Immunohistochemistry of iron handling proteins in healthy kidney. Representatives images of ZIP8 (a), ZIP14 (b), divalent metal transporter 1 (DMT1; c), L-ferritin (d), H-ferritin (e), & ferroportin (f) staining in healthy kidney. Renal structures indicated as glomerulus (g), proximal tubule (PT), distal tubule (DT). Scale bar 20 µM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29921869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004891-m01_mouse-monoclonal-nramp2-slc11a2-dmt1-antibody-4c6-310202415384187.jpg)
![Immunohistochemistry: NRAMP2/SLC11A2/DMT1 Antibody (4C6) [H00004891-M01] - Immunohistochemistry of putative iron importers in chronic kidney disease. Representative images of ZIP8 (a–f), ZIP14 (g–l), & divalent metal transporter 1 (DMT1; m–r) staining in control (a,g,m), early diabetic nephropathy (DNE; b,h,n), advanced diabetic nephropathy (DNA; c,i,o), focal segmental glomerulosclerosis (FSGS; d,j,p), lupus nephritis (LN; e,k,q) & IgA nephropathy (IgAN; f,l,r). Intensity in proximal & distal tubules quantified (s). Dots represent all quantified images (5 images per biopsy). Renal structures indicated as glomerulus (G), proximal tubule (PT), distal tubule (DT). Scale bar 40 µM. **p < 0.01; ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29921869), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00004891-m01_mouse-monoclonal-nramp2-slc11a2-dmt1-antibody-4c6-31020241534339.jpg)
![Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - KO Validation in MEF Cells.Loading: 10 u of lysate Antibodies: ADAM10, 1 u/mL and beta-actin, 1u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).](http://images.novusbio.com/fullsize/nbp1-76973_rabbit-polyclonal-adam10-antibody-2711202419392795.gif "<b>Genetic Strategies Validation. </b>Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - KO Validation in MEF Cells.Loading: 10 u of lysate Antibodies: ADAM10, 1 u/mL and beta-actin, 1u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).")
![<b>Genetic Strategies Validation. </b>Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - KO Validation in 293 Cells.Loading: 15 u of lysate Antibodies: ADAM10, 2 u/mL and beta-actin, 1u/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).](http://images.novusbio.com/fullsize/nbp1-76973_rabbit-polyclonal-adam10-antibody-2711202419241370.gif)
![Immunohistochemistry-Frozen: ADAM10 Antibody - BSA Free [NBP1-76973] - ADAM10 (green) stained human skin, counterstained with DAPI. Tissue fixed, permeablized, and blocked with 5% BSA TBS Ca/Azide. Anti-ADAM10 antibody applied at 1:100, before washed and stained with Alexa488-conjugated secondary. Slides mounted with DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize/ADAM10-Antibody-Immunohistochemistry-Frozen-NBP1-76973-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - Figure 8 Immunofluorescence Validation of ADAM10 in K562 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling ADAM10 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76973_rabbit-polyclonal-adam10-antibody-2711202420312914.jpg)
![Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - Figure 9 Immunofluorescence Validation of ADAM10 in Mouse TestisImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse testis labeling ADAM10 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76973_rabbit-polyclonal-adam10-antibody-271120242016920.jpg)
![Immunocytochemistry/ Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - Figure 10 Immunofluorescence Validation of ADAM10 in Rat Testis Immunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling ADAM10 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp1-76973_rabbit-polyclonal-adam10-antibody-271120241923654.jpg)
![Immunocytochemistry/Immunofluorescence: ADAM10 Antibody - BSA Free [NBP1-76973] - K562 cells with ADAM10 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/ADAM10-Antibody-Immunofluorescence-NBP1-76973-img0004.jpg)
![Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - Validation in Human Cell Lines.Loading: 15 u of lysate Antibodies: ADAM10, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).](http://images.novusbio.com/fullsize/nbp1-76973_rabbit-polyclonal-adam10-antibody-271120242016944.gif)
![Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - Validation in Rat Tissues.Loading: 15 u of lysates per lane.Antibodies: ADAM10, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).](http://images.novusbio.com/fullsize/nbp1-76973_rabbit-polyclonal-adam10-antibody-2711202419261856.gif)
![Western Blot: ADAM10 Antibody - BSA Free [NBP1-76973] - Validation in Mouse Tissues.Antibodies: ADAM10, 1 u/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Detected both precursor ADAM10 (94KD) and mature ADAM10 (68kD).](http://images.novusbio.com/fullsize/nbp1-76973_rabbit-polyclonal-adam10-antibody-27112024201319.gif)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0013.jpg "Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP")
![Immunohistochemistry: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420324853.gif)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0010.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0007.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0009.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0012.jpg)
![Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202419313214.gif)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420414588.jpg)
![Western Blot: LIMPII/SR-B2 Antibody [NBP1-77326] - Analysis in mouse liver tissue lysate with antibody at 1 ug/mL in (A) the absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Western-Blot-NBP1-77326-img0005.jpg "Western Blot: LIMPII/SR-B2 Antibody [NBP1-77326] - Analysis in mouse liver tissue lysate with antibody at 1 ug/mL in (A) the absence and (B) presence of blocking peptide.")
![Simple Western: LIMPII/SR-B2 Antibody [NBP1-77326] - Whole cell lysates from the indicated cell lines were probed, at the indicated concentrations, with a 1:25 dilution of antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/LIMPII-SR-B2-Antibody-Simple-Western-NBP1-77326-img0007.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody - BSA Free [NBP1-77326] - Immunohistochemistry of LIMPII/SR-B2 in human skeletal muscle tissue with LIMPII/SR-B2 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-77326_rabbit-polyclonal-limpii-sr-b2-antibody-2711202419451212.jpg)
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody - BSA Free [NBP1-77326] - Immunofluorescence of LIMPII/SR-B2 in Human Skeletal Muscle tissue with LIMPII/SR-B2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77326_rabbit-polyclonal-limpii-sr-b2-antibody-2711202419333447.jpg)
![Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/DARC-Antibody-Western-Blot-NB100-2421-img0007.jpg "Western Blot: DARC Antibody [NB100-2421] - DARC (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - Staining of paraffin embedded Human Lung. Microwaved antigen retrieval with citrate buffer Ph 6, AP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0009.jpg)
![Flow Cytometry: DARC Antibody [NB100-2421] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/DARC-Antibody-Flow-Cytometry-NB100-2421-img0010.jpg)
![Immunohistochemistry-Paraffin: DARC Antibody [NB100-2421] - DARC (3ug/ml) staining of paraffin embedded human kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/DARC-Antibody-Immunohistochemistry-Paraffin-NB100-2421-img0008.jpg)
![Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945] - IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain regions, including the hippocampus (HP), cortex (CTX) and paraventricular region (PVN). Image from verified customer review.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-Immunohistochemistry-NBP1-51945-img0008.jpg "Immunohistochemistry: CaM Kinase II alpha Antibody [NBP1-51945] - IHC using CamKII antibody from goat in 3% PFA perfused mouse brain. 1:100 dilution clearly provides cytosolic CamK II immunoreactivity in whole brain regions, including the hippocampus (HP), cortex (CTX) and paraventricular region (PVN). Image from verified customer review.")
![Flow Cytometry: CaM Kinase II alpha Antibody [NBP1-51945] - Flow cytometric analysis of paraformaldehyde fixed Kelly cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-18720231134294.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342929.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.75ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342911.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody [NBP1-51945] - (3.8ug/ml) staining of paraffin embedded Human Brain Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342919.jpg)
![Western Blot: CaM Kinase II alpha Antibody [NBP1-51945] - (0.3ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-18720231134296.jpg)
![Immunocytochemistry/Immunofluorescence: CaM Kinase II alpha Antibody [NBP1-51945] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).](http://images.novusbio.com/fullsize/nbp1-51945_goat-polyclonal-cam-kinase-ii-alpha-antibody-imgenex-img-31213-187202311342912.jpg)
![Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/SRD5A1-Antibody-Immunohistochemistry-Paraffin-NB100-1491-img0002.jpg "Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.")
![Immunohistochemistry-Paraffin: SRD5A1 Antibody [NB100-1491] - (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/SRD5A1-Antibody-Immunohistochemistry-Paraffin-NB100-1491-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: POLD3 Antibody (3E2) [H00010714-M01] - Analysis of monoclonal antibody to POLD3 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/POLD3-Antibody-3E2-Immunocytochemistry-Immunofluorescence-H00010714-M01-img0004.jpg "Immunocytochemistry/Immunofluorescence: POLD3 Antibody (3E2) [H00010714-M01] - Analysis of monoclonal antibody to POLD3 on HeLa cell . Antibody concentration 10 ug/ml.")
![ELISA: POLD3 Antibody (3E2) [H00010714-M01] - Detection limit for recombinant GST tagged POLD3 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/POLD3-Antibody-3E2-ELISA-H00010714-M01-img0006.jpg)
![Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Left: Uninfected cells. Right: Cells infected with yellow fever 17D virus, MOI=1. Image from verified customer review. Analysis using NBP2-52666F conjugate.](http://images.novusbio.com/fullsize/Flavivirus-group-antigen-Antibody-D1-4G2-4-15-4G2-Chimeric-Flow-Cytometry-NBP2-52666-img0005.jpg "Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Left: Uninfected cells. Right: Cells infected with yellow fever 17D virus, MOI=1. Image from verified customer review. Analysis using NBP2-52666F conjugate.")
![Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Left panel: uninfected VeroB4 cells stained with 4G2 antibody at 1:400. Right panel: VeroB4 cells infected with Yellow-Fever 17D-Venus virus, stained with 4G2 antibody at 1:400. The signal from the 4G2 antibody correlates well with the Venus signal from the virus. Image from verified customer review.](http://images.novusbio.com/fullsize/Flavivirus-group-antigen-Antibody-D1-4G2-4-15-4G2-Chimeric-Flow-Cytometry-NBP2-52666-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Detection of Zika virus. Immunofluorescence images of Vero cells infected with ZIKV after 30h infection as well as controls. Cells were fixed in 4% PFA for 30 min at RT. Primary antibody (Rabbit IgG) staining was performed in 0.3% Triton X-100 at 1:20 dilution overnight at 4C, detected using a Goat Anti-Rabbit IgG (1:500) for 1h at RT and counterstained with DAPI.](http://images.novusbio.com/fullsize/Flavivirus-group-antigen-Antibody-D1-4G2-4-15-4G2-Chimeric-Immunofluorescence-NBP2-52666-img0001.png)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Scanning electron microscopy (SEM) of FACS sorted B cells. P. alecto splenocytes were surface-stained for CD11b, MHC-II and Ig antibodies and sorted as CD11b- MHC-II+ sIg+ (B cells), CD11b+ (myeloid cells) and CD11b- MHC-II- sIg- cells (T cells). Sorted B cells were processed for viewing under a scanning electron microscope. Scanned electron micrograph of sorted Pteropus alecto MHC-II+sIg+ B cells. Bar represents 2 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.00489/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Goat-anti-Bat-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7237-img0002.jpg "Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Scanning electron microscopy (SEM) of FACS sorted B cells. P. alecto splenocytes were surface-stained for CD11b, MHC-II and Ig antibodies and sorted as CD11b- MHC-II+ sIg+ (B cells), CD11b+ (myeloid cells) and CD11b- MHC-II- sIg- cells (T cells). Sorted B cells were processed for viewing under a scanning electron microscope. Scanned electron micrograph of sorted Pteropus alecto MHC-II+sIg+ B cells. Bar represents 2 um. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2019.00489/full), licensed under a CC-BY license.")
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies and analysed by FACS. Strategy to identify B cells based on CD3- MHCII+ and IgG+ staining. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep37796), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Goat-anti-Bat-IgG-H+L-Secondary-Antibody-Flow-Cytometry-NB7237-img0001.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415395915.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241534359.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415175281.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241535683.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Intracellular & surface Ig staining of P. alecto splenocytes. Dead cells were excluded using Live/Dead eFluor 506 dye & live cells were gated for singlets using forward scatter height (FSC-H) & area (FSC-A). For all the staining approaches (surface only, intracellular only, or surface + intracellular), a distinct sIg+ CD3+/− population is observed. Greater than 85% of this population is MHCII+. Comparable percentages of these cells were obtained with surface staining only vs. intracellular staining only, thus strongly suggesting that this population is truly a sIg+ B cell population. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30930908), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415175251.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415345310.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415284518.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241534323.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415371931.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415345349.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241537191.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415392567.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Detection of T cell subsets by combined Flow-FISH & antibody staining.Bat splenocytes were stained with CD3, Tbet, Eomes & Gata3 antibodies followed by in-situ hybridization with probes specific for CD4mRNA (a) & CD8mRNA (b). One data set is shown & is representative of 2 independent experiments performed with 2 different bat spleens. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415291918.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415291938.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415175290.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415345340.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241539458.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Bat T cell production of cytokines & cytolytic factors upon mitogenic stimulation.Bat splenocytes (a–c,g–i) or PBMCs (d–f) were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Detection of intracellular TNF (a), IFN-gamma (b), IL-10 (c) was performed at the protein level, & production of IL- 2 (d), granzyme B (e), perforin (f), IL-17a (g), IL-22 (h) & TGF-beta 1 (i) was detected at the mRNA level by Flow-FISH. Dot plots obtained with one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415371920.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Strategy for immunophenotyping of lymphocytes in P. alecto.Bat splenocytes were stained with cross-reactive antibodies & analysed by FACS. (a) Gating strategy based on live cells, singlets & lymphocyte region. (b) CD3+ cell population analysed for the expression of transcription factors Gata3, Tbet & Eomes. (c) Strategy to identify B cells based on CD3− MHCII+ & IgG+ staining. (d) Strategy to identify NK cells based on CD3−, Eomes+ & Tbet+ staining. One set of data obtained from one bat spleen is shown, & is representative of 4 different bat spleens analyzed. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415291963.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-31020241536374.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Expression of MHCII molecules by CD3+ T cells.(a) FACS analysis of splenocytes from one bat based on detection of MHCII & CD3 molecules. (b) Individual percentages of MHCII+ T cells in spleen, MLN, PBMCs & BM from 3–4 bats. The mean (horizontal bar) & SEM are shown. (c) Dot plot featuring Tbet & Eomes expression in MHCII+ & MHCII− CD3+ splenocytes from one bat. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415363745.jpg)
![Flow Cytometry: Goat anti-Bat IgG (H+L) Secondary Antibody [NB7237] - Production of cytokines by B & NK cells upon mitogenic stimulation.Bat splenocytes or PBMCs were stimulated for 4 h with PDBu/ionomycin or media in the presence of brefeldin A & monensin. Cells were gated on CD3− MHCII+ (B cells) (a,b) or CD3− Tbet+ Eomes+ (NK cells). Production of intracellular TNF (a,c), IL-10 (b), IFN-gamma (d) was done at the protein level, & production of granzyme B & perforin (e) was performed at the mRNA level by Flow-FISH. Dot plots from one bat are shown & are representative of the results obtained with 2–3 different bats. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27883085), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb7237_goat-polyclonal-goat-anti-bat-igg-h-l-secondary-antibody-310202415371966.jpg)