Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric

Images

 
Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Left: Uninfected cells. Right: Cells infected with yellow fever 17D virus, MOI=1. Image from verified customer review. ...read more
Flow Cytometry: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Left panel: uninfected VeroB4 cells stained with 4G2 antibody at 1:400. Right panel: VeroB4 cells infected with ...read more
Immunofluorescence: Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric [NBP2-52666] - Detection of Zika virus. Immunofluorescence images of Vero cells infected with ZIKV after 30h infection as well as ...read more

Product Details

Summary
Reactivity V-ViSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P, B/N, CyTOF-ready, ICC/IF
Clone
D1-4G2-4-15 (4G2)
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
This recombinant Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric was prepared from Dengue Virus type 2 antigens.
Specificity
This Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric recognises flavivirus group specific antigens (Dengue virus, West Nile Virus, Japanese Encephalitis, Yellow Fever Virus, Zika virus etc). It binds to the fusion loop at the extremity of domain II of protein E.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • CyTOF-ready
  • Immunofluorescence 1:10 - 1:500
  • Neutralization
Application Notes
This antibody is CyTOF ready. This chimeric rabbit antibody was made using the variable domain sequences of the original murine IgG2a format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to flavivirus group antigen, protein E. It can be used as an anti-Dengue virus antibody, anti-West Nile virus antibody, anti-Japanese Encephalitis, anti-Yellow Fever Virus or anti-Zika Virus antibody (Aubry et al. 2016) to identify cells infected with these flaviviridae. It binds to the fusion loop at the extremity of domain II of E protein from all four serotypes and prevents syncytia formation (Summers, 1989). The epitope is highly conserved amongst flaviviridae and has been functionally analyzed in detail by Crill and Chang 2004 (PMID: 15564505). Previous studies have used acetone- (Henchal et al. 1982) or methanol-fixed slides (Moreland & Tay, 2010). Please note that binding of this antibody has been reported to be sensitive to reduction and Western Blots should be performed under non-reducing conditions (Lai et al. 2008). A version of 4G2 directly conjugaed to APC was used by Quicke and co-workers (Quicke, 2016) to detect Zika virus in human placental macrophages by flow-cytometry (Quicke 2016). Ramaiah et al. (2016) used NBP2-52709 at a 1:200 dilution to stain for Zika virus in methanol-fixed Vero cells 48 h post-infection.
Publications
Read Publications using
NBP2-52666 in the following applications:

  • 1 publication
  • 1 publication
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Dengue Virus, Zika Virus, West Nile Virus, Yellow Fever Virus, Flaviviridae

Packaging, Storage & Formulations

Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Buffer
PBS
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric

  • Envelope Protein
  • Protein E

Background

Flaviviruses are a group of seventy enveloped, positive-sense single stranded RNA viruses belonging to the Flaviviridae family (1-5). Flaviviruses are typically transmitted through arthropods, like mosquitoes and ticks, and can infect humans causing outbreaks and serious illness or death worldwide (1-5). These well-known viral pathogens include dengue virus (DENV), West Nile virus (WNV), Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBEV), Yellow Fever virus (YFV), and Zika virus (ZIKV) (1-5). Flaviviruses consist of ~10.7 kb RNA genome with one open reading frame (ORF) that is flanked by 5' and 3' untranslated regions (UTRs) (1, 2, 6). The ORF encodes for a polyprotein that is made up of three structural proteins, the capsid (C), premembrane (prM), and envelope (E) proteins, and seven non-structural (NS) proteins (NS1, NS2A/B, NS3, NS4A, 2K, NS4B, and NS5) (1, 2, 5, 6).

The Flavivirus group antigen antibody binds to the fusion loop at the distal end of domain II (EDII) of protein E. Flavivirus E is a glycoprotein that is the main antigen for membrane fusion between virus and host and entry into the cell, as well as inducing protective immunity by stimulating neutralizing antibodies (2, 4, 6). The E protein is 495 amino acids (aa) in length and has a theoretical molecular weight between 55 - 72 kDa, which varies based on glycosylation sites (2). Flavivirus E protein contains three domains (EDI, EDII, and EDIII) followed by a stem that contents to the helix-transmembrane domains (TMDs, TM1 and TM2) (2, 4, 6). The EDII contains an S-S bridge at its distal end and functions as a fusion loop (FL) for virus-mediated membrane fusion and antibody binding (2). Given the important role of Flavivirus protein E in host cell infection and membrane fusion, it has become a target for flavivirus disease prevention and treatment (2, 5).

Alternative names for Flavivirus group antigen include Envelope Protein and Protein E.

References

1. Ngono, A. E., & Shresta, S. (2018). Immune Response to Dengue and Zika. Annual review of immunology. https://doi.org/10.1146/annurev-immunol-042617-053142

2. Rey, F. A., Stiasny, K., Vaney, M. C., Dellarole, M., & Heinz, F. X. (2018). The bright and the dark side of human antibody responses to flaviviruses: lessons for vaccine design. EMBO reports. https://doi.org/10.15252/embr.201745302

3. Zhang, X., Jia, R., Shen, H., Wang, M., Yin, Z., & Cheng, A. (2017). Structures and Functions of the Envelope Glycoprotein in Flavivirus Infections. Viruses. https://doi.org/10.3390/v9110338

4. Laureti, M., Narayanan, D., Rodriguez-Andres, J., Fazakerley, J. K., & Kedzierski, L. (2018). Flavivirus Receptors: Diversity, Identity, and Cell Entry. Frontiers in immunology. https://doi.org/10.3389/fimmu.2018.02180

5. Boldescu, V., Behnam, M., Vasilakis, N., & Klein, C. D. (2017). Broad-spectrum agents for flaviviral infections: dengue, Zika and beyond. Nature reviews. Drug discovery. https://doi.org/10.1038/nrd.2017.33

6. Crill, W. D., & Chang, G. J. (2004). Localization and characterization of flavivirus envelope glycoprotein cross-reactive epitopes. Journal of virology, 78(24), 13975-13986. https://doi.org/10.1128/JVI.78.24.13975-13986.2004

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Flavivirus group antigen Antibody (NBP2-52666)(7)

We have publications tested in 3 confirmed species: Human, Primate, Virus.

We have publications tested in 4 applications: Flow, Func, IHC-P, WB.


Filter By Application
Flow
(1)
Func
(1)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Primate
(1)
Virus
(3)
All Species

Reviews for Flavivirus group antigen Antibody (NBP2-52666) (0)

There are no reviews for Flavivirus group antigen Antibody (NBP2-52666). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Flavivirus group antigen Antibody (NBP2-52666) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52666AF350
Alexa Fluor 405 NBP2-52666AF405
Alexa Fluor 488 NBP2-52666AF488
Alexa Fluor 532 NBP2-52666AF532
Alexa Fluor 594 NBP2-52666AF594
Alexa Fluor 647 NBP2-52666AF647
Alexa Fluor 700 NBP2-52666AF700
Alexa Fluor 750 NBP2-52666AF750
Allophycocyanin NBP2-52666APC
Allophycocyanin/Cy7 NBP2-52666APCCY7
Biotin NBP2-52666B
DyLight 350 NBP2-52666UV
DyLight 405 NBP2-52666V
DyLight 488 NBP2-52666G
DyLight 550 NBP2-52666R
DyLight 594 NBP2-52666DL594
DyLight 650 NBP2-52666C
DyLight 680 NBP2-52666FR
DyLight 755 NBP2-52666IR
FITC NBP2-52666F
HRP NBP2-52666H
Janelia Fluor 549 NBP2-52666JF549
Janelia Fluor 646 NBP2-52666JF646
PE NBP2-52666PE
PE/Atto594 NBP2-52666PEATT594
PE/Cy5.5 NBP2-52666PECY55
PE/Cy7 NBP2-52666PECY7
PerCP NBP2-52666PCP

Additional Flavivirus group antigen Products

Research Areas for Flavivirus group antigen Antibody (NBP2-52666)

Find related products by research area.

Blogs on Flavivirus group antigen

There are no specific blogs for Flavivirus group antigen, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Flavivirus group antigen Antibody (D1-4G2-4-15 (4G2)) - Chimeric and receive a gift card or discount.