Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alkaline Phosphatase | NBP1-75294 | |||
| Biotin | NBP1-75295 | |||
| DyLight 350 | NBP1-76054 | |||
| DyLight 488 | NBP1-75299 | |||
| DyLight 550 | NBP1-76055 | |||
| DyLight 594 | NBP1-76056 | |||
| DyLight 633 | NBP1-76057 | |||
| DyLight 650 | NBP1-76058 | |||
| FITC | NBP1-75296 | |||
| HRP | NBP1-75297 | |||
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of goat anti-rabbit IgG-HRP secondary antibody (NBP1-75297).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0007.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of goat anti-rabbit IgG-HRP secondary antibody (NBP1-75297).")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Lysates of Jurkat human acute T cell leukemia cell line either untreated (-) or treated (+) with EPE. PVDF membrane was probed with 0.25 ug/mL rabbit anti-phospho RAD17 (S635) monoclonal antibody (MAB1374) followed by 1:1000 dilution of Goat anti-Rabbit IgG (H+L) Secondary Antibody (NBP1-75297).](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0005.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [NBP1-75293] - Analysis of WNV-E in A549 cell lysate using Goat anti-Rabbit IgG (H+L) Secondary Antibody. The secondary antibody was diluted in 5% milk at a dilution of 1:3000. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-Western-Blot-NBP1-75293-img0004.jpg)
![N/A IL-12/IL-23 p40 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL12_p40_DY2398_ELISA_1822.jpg "N/A IL-12/IL-23 p40 [Biotin]")
![N/A IFN-gamma [Biotin]](http://images.novusbio.com/fullsize2/IFN-gamma_CAIF00_ELISA_7.jpg "N/A IFN-gamma [Biotin]")
![N/A IFN-gamma [Biotin]](http://images.novusbio.com/fullsize2/caif00_canine-ifn-gamma-quantikine-elisa-kit-1392021105113.jpg)
![N/A Growth Hormone [HRP]](http://images.novusbio.com/fullsize2/DATA_Growth_Hormone_DGH00_ELISA_700.jpg "N/A Growth Hormone [HRP]")
![N/A Growth Hormone [HRP]](http://images.novusbio.com/fullsize2/DATA_Growth_Hormone_DGH00_ELISA_701.jpg)
![N/A Growth Hormone [HRP]](http://images.novusbio.com/fullsize2/Growth_Hormone_DGH00_ELISA_109.jpg)
![Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VDAC1-Antibody-Western-Blot-NB100-695-img0005.jpg "Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Subcellular localization of BNIP3 phosphomutants, showing Western blot of cytosolic and mitochondrial fractions. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY license.")
![Western Blot: VDAC1 Antibody [NB100-695] - Lane 1: rat heart whole cell lysate. Lane 2: none. Load: 35 ug per lane. Primary antibody: VDAC/Porin antibody at 1:1,200 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~32 kDa corresponding to VDAC/Porin (arrowhead).](http://images.novusbio.com/fullsize/VDAC1-Antibody-Western-Blot-NB100-695-img0004.jpg)
![Western Blot: VDAC1 Antibody [NB100-695] - The BNIP3 C-terminus can be phosphorylated. Alkaline extraction of mitochondria-associated proteins, showing alkaline extract of the mitochondrial pellet and the alkaline-resistant mitochondrial pellet from cells expressing each BNIP3 phosphomutant. All blots are representative of at least 3 independent experiments. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0129667), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/VDAC1-Antibody-Western-Blot-NB100-695-img0006.jpg)
![N/A TFPI [HRP]](http://images.novusbio.com/fullsize2/DATA_TFPI_DTFP10_ELISA_841.jpg "N/A TFPI [HRP]")
![N/A TFPI [HRP]](http://images.novusbio.com/fullsize2/TFPI_DTFP10_ELISA_190.jpg)
![N/A IL-3 [HRP]](http://images.novusbio.com/fullsize2/DATA_IL3_M3000_ELISA_931.jpg "N/A IL-3 [HRP]")
![N/A IL-3 [HRP]](http://images.novusbio.com/fullsize2/IL-3_M3000_ELISA_234.jpg)
![N/A VEGF [Biotin]](http://images.novusbio.com/fullsize2/DATA_VEGF_DY3045_ELISA_1908.jpg "N/A VEGF [Biotin]")
![N/A Gas6 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Gas6_DY986_ELISA_2293.jpg "N/A Gas6 [Biotin]")
![N/A MMP-3 [HRP]](http://images.novusbio.com/fullsize2/DATA_MMP3_MMP300_ELISA_1004.jpg "N/A MMP-3 [HRP]")
![N/A MMP-3 [HRP]](http://images.novusbio.com/fullsize2/MMP-3_MMP300_ELISA_295.jpg)
![N/A IGFBP-2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IGFBP2_DY674_ELISA_2173.jpg "N/A IGFBP-2 [Biotin]")