Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76933-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22340SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41269-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76645-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76782-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36445-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77242-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77169-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41274-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76911-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41266-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-75518-0.025mg | |||
![Western Blot: ASAH1 Antibody [NBP1-76933] - Analysis in human heart tissue lysate with antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ASAH1-Antibody-Western-Blot-NBP1-76933-img0005.jpg "Western Blot: ASAH1 Antibody [NBP1-76933] - Analysis in human heart tissue lysate with antibody at 1 ug/mL.")
![Immunohistochemistry: ASAH1 Antibody - BSA Free [NBP1-76933] - Immunohistochemistry of ASAH1 in rat heart tissue with ASAH1 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76933_rabbit-polyclonal-asah1-antibody-271120242022926.gif)
![Immunocytochemistry/ Immunofluorescence: ASAH1 Antibody - BSA Free [NBP1-76933] - Immunofluorescence of ASAH1 in Human Heart cells with ASAH1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76933_rabbit-polyclonal-asah1-antibody-2711202420195360.jpg)
![Immunohistochemistry: ASAH1 Antibody - BSA Free [NBP1-76933] - Immunofluorescence of ASAH1 in rat heart tissue with ASAH1 antibody at 20 u/mL.Red: ASAH1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76933_rabbit-polyclonal-asah1-antibody-2711202420243335.gif)
![Immunohistochemistry: ASAH1 Antibody - BSA Free [NBP1-76933] - Immunohistochemistry of ASAH1 in human heart tissue with ASAH1 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76933_rabbit-polyclonal-asah1-antibody-271120242092039.jpg)
![Western Blot: SHISA3 Antibody [NBP2-22340] - Analysis SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/SHISA3-Antibody-Western-Blot-NBP2-22340-img0002.jpg "Western Blot: SHISA3 Antibody [NBP2-22340] - Analysis SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: SHISA3 Antibody [NBP2-22340] - SHISA3 in human small intestine tissue with SHISA3 antibody at 2.5 ug/ml.](http://images.novusbio.com/fullsize/SHISA3-Antibody-Immunohistochemistry-NBP2-22340-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: SHISA3 Antibody - BSA Free [NBP2-22340] - Immunofluorescence of SHISA3 in human small intestine tissue with SHISA3 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-22340_rabbit-polyclonal-shisa3-antibody-2711202419293855.jpg)
![Western Blot: Olig2 Antibody [NBP2-41269] - Western blot analysis of OLIG2 in EL4 cell lysate with OLIG2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Olig2-Antibody-Western-Blot-NBP2-41269-img0001.jpg "Western Blot: Olig2 Antibody [NBP2-41269] - Western blot analysis of OLIG2 in EL4 cell lysate with OLIG2 antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry: Olig2 Antibody [NBP2-41269] - Staining of OLIG2 in mouse brain tissue with OLIG2 Antibodyat 5 ug/mL.](http://images.novusbio.com/fullsize/Olig2-Antibody-Immunohistochemistry-NBP2-41269-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - BSA Free [NBP2-41269] - Immunofluorescence of Olig2 in mouse brain tissue with Olig2 Antibody at 20 ug/mL.Green: Olig2 antibody Red: Phylloidin staining Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-41269_rabbit-polyclonal-olig2-antibody-271120242045633.gif)
![Immunocytochemistry/ Immunofluorescence: Olig2 Antibody - BSA Free [NBP2-41269] - Immunofluorescence of Olig2 in rat brain tissue with Olig2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41269_rabbit-polyclonal-olig2-antibody-2711202420285837.jpg)
![Immunohistochemistry: Olig2 Antibody - BSA Free [NBP2-41269] - Immunohistochemistry of Olig2 in rat brain tissue with Olig2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41269_rabbit-polyclonal-olig2-antibody-2711202419432216.jpg)
![Western Blot: GRG (Groucho homolog) Antibody [NBP1-76645] - Analysis of GRG (Groucho homolog) in 293 cell lysate with GRG (Groucho homolog) antibody using NBP1-76645 at (A) 1, (B) 2 and (C) 4 ug/ml.](http://images.novusbio.com/fullsize/GRG-Groucho-homolog-Antibody-Western-Blot-NBP1-76645-img0005.jpg "Western Blot: GRG (Groucho homolog) Antibody [NBP1-76645] - Analysis of GRG (Groucho homolog) in 293 cell lysate with GRG (Groucho homolog) antibody using NBP1-76645 at (A) 1, (B) 2 and (C) 4 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunofluorescence of GRG (Groucho homolog) in Hela cells with GRG (Groucho homolog) antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76645_rabbit-polyclonal-grg-groucho-homolog-antibody-2711202419475890.jpg)
![Immunocytochemistry/ Immunofluorescence: GRG (Groucho homolog) Antibody - BSA Free [NBP1-76645] - Immunocytochemistry of GRG (Groucho homolog) in HeLa cells with GRG (Groucho homolog) antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76645_rabbit-polyclonal-grg-groucho-homolog-antibody-271120242022923.jpg)
![Western Blot: IRAK3 Antibody [NBP1-76782] - Analysis of IRAK3 in mouse spleen (M) and rat liver (R) tissue lysates with IRAK3 antibody using this antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Western-Blot-NBP1-76782-img0003.jpg "Western Blot: IRAK3 Antibody [NBP1-76782] - Analysis of IRAK3 in mouse spleen (M) and rat liver (R) tissue lysates with IRAK3 antibody using this antibody at 1 ug/ml.")
![Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-76782] - Staining of human spleen tissue with antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunohistochemistry-Paraffin-NBP1-76782-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 ug/mL.Red: IRAK3 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76782_rabbit-polyclonal-irak3-antibody-271120242013232.gif)
![Immunohistochemistry: IRAK3 Antibody [NBP1-76782] - Rat Liver tissue with IRAK-M antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/IRAK3-Antibody-Immunofluorescence-NBP1-76782-img0004.jpg)
![Immunohistochemistry: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunohistochemical staining of rat liver tissue using IRAK3 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76782_rabbit-polyclonal-irak3-antibody-2711202419541592.jpg)
![Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target of rapamycin (mTOR), ULK1, p-GSK3Beta (Ser9), GSK3Beta in extracts of whole brain tissue were detected by WB. Representative Western blots are presented in panels, and quantification of the results is shown as the mean values +- SD in panels (B,D,F,H). *p less than 0.05, **p less than 0.01, and ***p less than 0.001 between two groups. One-way analysis of variance/Newman-Keuls test was performed for all WB data. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fnagi.2020.00047/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ATG14-Antibody-Western-Blot-NBP2-36445-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - DHA increases the basal level of autophagy. Protein levels of ATG5, ATG12, ATG16L1, Beclin1, ATG14, p-ATG14 (Ser29), Rab7, RILP, Lamp1, CTSB, p-mTOR(Ser2448), mammalian target of rapamycin (mTOR), ULK1, p-GSK3Beta (Ser9), GSK3Beta in extracts of whole brain tissue were detected by WB. Representative Western blots are presented in panels, and quantification of the results is shown as the mean values +- SD in panels (B,D,F,H). *p less than 0.05, **p less than 0.01, and ***p less than 0.001 between two groups. One-way analysis of variance/Newman-Keuls test was performed for all WB data. Image collected and cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fnagi.2020.00047/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ATG14 Antibody - BSA Free [NBP2-36445] - ATG14 Antibody [NBP2-36445] - Analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ATG14-Antibody-Western-Blot-NBP2-36445-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG14 Antibody - BSA Free [NBP2-36445] - Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-36445_rabbit-polyclonal-atg14-antibody-2711202420285856.jpg)
![Western Blot: PIST Antibody [NBP1-77242] - Analysis in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/mL.](http://images.novusbio.com/fullsize/PIST-Antibody-Western-Blot-NBP1-77242-img0004.jpg "Western Blot: PIST Antibody [NBP1-77242] - Analysis in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/mL.")
![Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunohistochemistry of PIST in human testis tissue with PIST antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-2711202419531635.gif)
![Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-2711202419463455.jpg)
![Immunohistochemistry: PIST Antibody - BSA Free [NBP1-77242] - Immunofluorescence of PIST in human testis tissue with PIST antibody at 20 ug/ml.Green: PIST Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-2711202420195363.gif)
![Immunocytochemistry/ Immunofluorescence: PIST Antibody - BSA Free [NBP1-77242] - Immunofluorescence of PIST in Rat Colon cells with PIST antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77242_rabbit-polyclonal-pist-antibody-271120242013170.jpg)
![Western Blot: ATG9B Antibody [NBP1-77169] - HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/ATG9B-Antibody-Western-Blot-NBP1-77169-img0001.jpg "Western Blot: ATG9B Antibody [NBP1-77169] - HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] - Immunofluorescence of ATG9B in Hela cells with ATG9B antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77169_rabbit-polyclonal-atg9b-antibody-2711202419313264.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG9B Antibody - BSA Free [NBP1-77169] - Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-77169_rabbit-polyclonal-atg9b-antibody-2711202420212356.jpg)
![Western Blot: Vinculin Antibody [NBP2-41274] - Western Blot Validation in PC3 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: Vinculin NBP2-41274 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41274-img0007.jpg "Western Blot: Vinculin Antibody [NBP2-41274] - Western Blot Validation in PC3 Cell Lysate. Loading: 15 ug of lysates per lane.Antibodies: Vinculin NBP2-41274 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![Immunocytochemistry/ Immunofluorescence: Vinculin Antibody - BSA Free [NBP2-41274] - Validation of Vinculin in Jurkat Cells. Immunocytochemical analysis of Jurkat cells using anti-Vinculin antibody at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41274_rabbit-polyclonal-vinculin-antibody-2711202420352440.gif)
![<b>Independent Antibodies Validation. </b>Western Blot: Vinculin Antibody [NBP2-41274] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41237 (2 ug/mL), Vinculin from Company B (1 ug/mL), Vinculin NBP2-41274 (1 ug/mL), and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41274-img0005.jpg)
![Western Blot: Vinculin Antibody [NBP2-41274] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Vinculin NBP2-41274 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/Vinculin-Antibody-Western-Blot-NBP2-41274-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: Vinculin Antibody - BSA Free [NBP2-41274] - Validation of Vinculin in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp2-41274_rabbit-polyclonal-vinculin-antibody-2711202419241340.gif)
![Western Blot: GKAP/DLGAP1 Antibody [NBP1-76911] - Analysis in rat brain tissue lysate with antibody at (A) 0.5 and (B) 1 ug/mL.](http://images.novusbio.com/fullsize/GKAP-DLGAP1-Antibody-Western-Blot-NBP1-76911-img0004.jpg "Western Blot: GKAP/DLGAP1 Antibody [NBP1-76911] - Analysis in rat brain tissue lysate with antibody at (A) 0.5 and (B) 1 ug/mL.")
![Immunohistochemistry: GKAP/DLGAP1 Antibody - BSA Free [NBP1-76911] - Immunohistochemistry of GKAP/DLGAP1 in human brain with GKAP/DLGAP1 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76911_rabbit-polyclonal-gkap-dlgap1-antibody-271120241935388.jpg)
![Immunocytochemistry/ Immunofluorescence: GKAP/DLGAP1 Antibody - BSA Free [NBP1-76911] - Immunofluorescence of GKAP/DLGAP1 in Human Brain cells with GKAP/DLGAP1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76911_rabbit-polyclonal-gkap-dlgap1-antibody-2711202419251521.jpg)
![Immunohistochemistry: TAF9 Antibody [NBP2-41266] - TAF9 in mouse liver tissue with TAF9 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/TAF9-Antibody-Immunohistochemistry-NBP2-41266-img0002.jpg "Immunohistochemistry: TAF9 Antibody [NBP2-41266] - TAF9 in mouse liver tissue with TAF9 antibody at 5 ug/mL.")
![Immunohistochemistry: TAF9 Antibody - BSA Free [NBP2-41266] - Immunohistochemistry of TAF9 in mouse liver tissue with TAF9 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41266_rabbit-polyclonal-taf9-antibody-2711202419251573.jpg)
![Immunocytochemistry/ Immunofluorescence: TAF9 Antibody - BSA Free [NBP2-41266] - Immunofluorescence of TAF9 in mouse liver tissue with TAF9 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41266_rabbit-polyclonal-taf9-antibody-271120241918639.jpg)
![Immunohistochemistry: TAF9 Antibody - BSA Free [NBP2-41266] - Immunohistochemistry of TAF9 in mouse liver tissue with TAF9 antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41266_rabbit-polyclonal-taf9-antibody-2711202420324863.jpg)
![Immunocytochemistry/ Immunofluorescence: TAF9 Antibody - BSA Free [NBP2-41266] - Immunofluorescence of TAF9 in mouse liver tissue with TAF9 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41266_rabbit-polyclonal-taf9-antibody-2711202420324840.jpg)
![Western Blot: PD-1 Antibody (7A11B1) [NBP1-75518] - A-20 cell lysate with PD-1 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking recombinant protein.](http://images.novusbio.com/fullsize/PD-1-Antibody-7A11B1-Western-Blot-NBP1-75518-img0006.jpg "Western Blot: PD-1 Antibody (7A11B1) [NBP1-75518] - A-20 cell lysate with PD-1 antibody at 1 ug/mL in the (A) absence and (B) presence of blocking recombinant protein.")
![Immunohistochemistry: PD-1 Antibody (7A11B1) - BSA Free [NBP1-75518] - Immunohistochemistry of PD-1 in mouse brain tissue with PD-1 antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp1-75518_mouse-monoclonal-pd-1-antibody-7a11b1-271120242022929.jpg)
![Immunocytochemistry/ Immunofluorescence: PD-1 Antibody (7A11B1) - BSA Free [NBP1-75518] - Immunofluorescence of PD-1 in human spleen tissue with PD-1 antibody at 20 u/ml.Green: PD-1 Antibody [7A11B1] Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-75518_mouse-monoclonal-pd-1-antibody-7a11b1-2711202420441127.gif)
![Immunohistochemistry: PD-1 Antibody (7A11B1) - BSA Free [NBP1-75518] - Immunohistochemistry of PD-1 in human spleen tissue with PD-1 antibody at 25 u/ml.](http://images.novusbio.com/fullsize/nbp1-75518_mouse-monoclonal-pd-1-antibody-7a11b1-27112024204569.gif)
![Immunocytochemistry/ Immunofluorescence: PD-1 Antibody (7A11B1) - BSA Free [NBP1-75518] - Immunofluorescence of PD-1 in mouse brain tissue with PD-1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-75518_mouse-monoclonal-pd-1-antibody-7a11b1-2711202420175132.jpg)