SHISA3 Antibody - BSA Free

Western Blot: SHISA3 Antibody [NBP2-22340] - Analysis SHISA3 in human small intestine Tissue lysate with SHISA3 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry: SHISA3 Antibody [NBP2-22340] - SHISA3 in human small intestine tissue with SHISA3 antibody at 2.5 ug/ml.

Immunocytochemistry/ Immunofluorescence: SHISA3 Antibody - BSA Free [NBP2-22340] - Immunofluorescence of SHISA3 in human small intestine tissue with SHISA3 antibody at 20 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SHISA3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SHISA3 Antibody - BSA Free (NBP2-22340) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-SHISA3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 15 amino acid peptide near the center of human SHISA3 . The immunogen is located within amino acids 120 - 170 of SHISA3. Amino Acid Squence: AAQPIRFSLRSYQTETL
• Specificity: SHISA3 antibody is predicted to not cross-react with other SHISA protein family members.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SHISA3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1 ug/ml
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SHISA3 Antibody - BSA Free

• hShisa3
• protein shisa-3 homolog
• shisa homolog 3 (Xenopus laevis)

Background

SHISA3 plays an essential role in the maturation of presomitic mesoderm cells by individual attenuation of both FGFand WNT signaling

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SHISA3 Antibody (NBP2-22340)(1)

Publication using NBP2-22340

• Muhl L, Mocci G, PietilA R et al. A single-cell transcriptomic inventory of murine smooth muscle cells Developmental cell 2022-10-24 [PMID: 36283392] (IF/IHC, Mouse)

Review for SHISA3 Antibody (NBP2-22340) (1)

Average Rating: 1

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer WB Mouse 12/07/2021

Summary

• Application: Western Blot
• Sample Tested: Bone marrow-derived macrophages
• Species: Mouse
• Lot: 7275-1203

Comments

• Comments: ***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.*** Mouse bone-marrow-derived macrophages were stimulated with 100 ng/ml for 2, 3, 4, 6, 8, 12, 16, 20, and 24 h or left unstimulated as control, label as 0 h; in DMEM+10% FBS, and lysed in lysis buffer (20 mM HEPES, pH7.4, 50 mM beta-glycerol phosphate, 2 mM EGTA, 1 mM DTT, 10 mM NaF, 1 mM Sodium Orthovanadate, 1% Triton-100 , 10% Glycerol, and various protease and phosphatase inhibitors). Lysates were resoled on 4-12% NuPAGE gel under denature condition. After transfering to nitrocellulose membrane, the blot was blocked in TBST containing 5% (w/v) Blotting Grade Blocker Non Fat Dry Milk (Bio-Rad) overnight at 4C. After blocking, the membrane was incubated with the primary antibody diluted to 1 ug/ml in TBST containing 5% BSA and 0.02% sodium azide for 1 h at room temperature or 60 h at 4 C. After 3 washes with TBST (5', 10' and 15') the blot was incubated with HRP-conjugated goat anti-rabbit secondary antibody (1:15,000) for 1 h at room temperature. After washing 3 times with TBST, the image was developed using ECL.

Bioinformatics

• Gene Symbol: SHISA3
• Uniprot:
  • NP_001073974(Human)