Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB100501-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14072 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB62153-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB97203-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75787 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76658-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77009-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76755-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77198-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76805-0.025mg | |||
![Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - Analysis of EPOR expression in transfected 293T cell line by EPOR monoclonal antibody (M01), clone 3D10.Lane 1: EPOR transfected lysate (Predicted MW: 55.1 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Erythropoietin-R-Antibody-3D10-Western-Blot-H00002057-M01-img0010.jpg "Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - Analysis of EPOR expression in transfected 293T cell line by EPOR monoclonal antibody (M01), clone 3D10.Lane 1: EPOR transfected lysate (Predicted MW: 55.1 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - Western Blot analysis of EPOR expression in different cell lines.](http://images.novusbio.com/fullsize/Erythropoietin-R-Antibody-3D10-Western-Blot-H00002057-M01-img0003.jpg)
![Western Blot: Erythropoietin R Antibody (3D10) [H00002057-M01] - EPOR monoclonal antibody (M01), clone 3D10. Analysis of EPOR expression in HeLa.](http://images.novusbio.com/fullsize/Erythropoietin-R-Antibody-3D10-Western-Blot-H00002057-M01-img0009.jpg)
![Immunoprecipitation: Erythropoietin R Antibody (3D10) [H00002057-M01] - Analysis of EPOR transfected lysate using anti-EPOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EPOR MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Erythropoietin-R-Antibody-3D10-Immunoprecipitation-H00002057-M01-img0007.jpg)
![ELISA: Erythropoietin R Antibody (3D10) [H00002057-M01] - Detection limit for recombinant GST tagged EPOR is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Erythropoietin-R-Antibody-3D10-ELISA-H00002057-M01-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Immunocytochemistry-Immunofluorescence-NBP3-13817-img0007.jpg "Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed MCF7 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).")
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed MCF7 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of PFA-fixed U87 cells. Staining localized to the nucleoplasm using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Immunocytochemistry-Immunofluorescence-NBP3-13817-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Immunofluorescence Analysis of K562 cells stained using QKI/Quaking Antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (green). CF640R phalloidin (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Immunocytochemistry-Immunofluorescence-NBP3-13817-img0006.jpg)
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed HeLa cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0001.jpg)
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed K562 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0002.jpg)
![Flow Cytometry: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Flow cytometric analysis of PFA-fixed U87 cells. QKI/Quaking antibody (PCRP-QKI-2F10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Flow-Cytometry-NBP3-13817-img0003.jpg)
![Protein Array: QKI/Quaking Antibody (PCRP-QKI-2F10) [NBP3-13817] - Analysis of Protein Array containing more than 19,000 full-length human proteins using QKI/Quaking Antibody (PCRP-QKI-2F10).](http://images.novusbio.com/fullsize/QKI-Quaking-Antibody-PCRP-QKI-2F10-Protein-Array-NBP3-13817-img0009.jpg)
![ELISA: Mouse VEGF-C ELISA Kit (Colorimetric) [NBP2-78893] - Samples were spiked with high concentrations of Mouse VEGF-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-78893_mouse-vegf-c-elisa-kit-colorimetric-132202416254038.jpg "ELISA: Mouse VEGF-C ELISA Kit (Colorimetric) [NBP2-78893] - Samples were spiked with high concentrations of Mouse VEGF-C and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse VEGF-C ELISA Kit (Colorimetric) [NBP2-78893] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-78893_mouse-vegf-c-elisa-kit-colorimetric-432024859725.jpg)
![Western Blot: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Western Blot Analysis of Human A431 cell lysate using Glut1 Antibody (GLUT1/2476).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Western-Blot-NBP2-75785-img0007.jpg "Western Blot: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Western Blot Analysis of Human A431 cell lysate using Glut1 Antibody (GLUT1/2476).")
![Immunocytochemistry/Immunofluorescence: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Immunofluorescence staining of MCF-7 cells using Glut1 Antibody (GLUT1/2476) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Immunocytochemistry-Immunofluorescence-NBP2-75785-img0005.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2476).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Immunohistochemistry-Paraffin-NBP2-75785-img0002.jpg)
![Flow Cytometry: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Flow Cytometric Analysis of K562 cells using Glut1 Antibody (GLUT1/2476) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Flow-Cytometry-NBP2-75785-img0004.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma stained with GLUT-1 Mouse Monoclonal Antibody (GLUT1/2476).](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Immunohistochemistry-Paraffin-NBP2-75785-img0001.jpg)
![Protein Array: Glut1 Antibody (GLUT1/2476) [NBP2-75785] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Glut1-Antibody-GLUT1-2476-Protein-Array-NBP2-75785-img0006.jpg)
![Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Bmf Antibody [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Bmf-Antibody-Western-Blot-NBP1-76658-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Bmf Antibody [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs were incubated with M-CSF (30 ng/ml) and RANKL (40 ng/ml) for 40 h, washed thoroughly, and incubated further with LPS (50 ng/ml) in the presence of M-CSF (30 ng/ml) for 48 h. Cells were transfected with 30 nM of miR-29b mimic or con mimic in the presence of M-CSF (30 ng/ml) for 6 h. Total RNA was analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, and HRK. Expression levels with con mimic treatment were set at 1. Cell lysates were subjected to Western blot analysis with antibodies against BMF, PUMA, BAK1, BIM, HRK, and BCL-2. Antibodies against beta-actin were used for normalization. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/omcl/2019/6018180/) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: Bmf Antibody [NBP1-76658] - Analysis of Bmf in 293 cell lysate with Bmf antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Bmf-Antibody-Western-Blot-NBP1-76658-img0005.jpg)
![Western Blot: Bmf Antibody - BSA Free [NBP1-76658] - Identification of target for LPS-induced miR-29b in OCs. BMMs incubated w/ M-CSF (30 ng/ml) & RANKL (40 ng/ml) for 40 h, washed thoroughly, & incubated further w/ LPS (50 ng/ml) in presence of M-CSF (30 ng/ml) for 48 h. (a) Cells transfected w/ 30 nM of miR-29b mimic or con mimic in presence of M-CSF (30 ng/ml) for 6 h. Total RNA analyzed by qPCR to quantify the expression of BMF, PUMA, BAK1, BIM, & HRK. Expression levels w/ con mimic treatment set at 1. Cell lysates subjected to WB analysis w/ antibodies against BMF, PUMA, BAK1, BIM, HRK, & BCL-2. Antibodies against beta -actin used for normalization. (b) w/out transfection, total RNA analyzed by qPCR to quantify the expression of BMF, & cell lysates subjected to WB analysis w/ anti-BMF Ab. (c) Total RNA & tissue lysate of tibiae from LPS-treated or vehicle-treated (V, PBS) mice analyzed by qPCR to quantify the expression of BMF & subjected to WB analysis w/ antibodies against BMF. (d) Cells thoroughly washed, transfected w/ 30 nM of anti-miR-29b or con inh, & stimulated w/ LPS (50 ng/ml) in presence of M-CSF. After 48 h, total RNA analyzed by qPCR to quantify BMF expression. Expression levels w/ con inh treatment set at 1.0. Cell lysates subjected to WB analysis w/ anti-BMF & anti-BCL-2 Ab. (e) Cells thoroughly washed, transfected w/ 50 nM of scRNA or siBMF, stimulated w/ LPS (50 ng/ml) in presence of M-CSF for 48 h, & analyzed to measure TRAP-positive MNCs & annexin V-positive cells. siRNA-mediated silencing of BMF confirmed by RT-PCR & qPCR. The Ct values of genes widely distributed between 17.33 & 30.92. ∗p Red: Bmf Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76658_rabbit-polyclonal-bmf-antibody-2711202419432229.gif)
![Immunocytochemistry/ Immunofluorescence: Bmf Antibody - BSA Free [NBP1-76658] - Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76658_rabbit-polyclonal-bmf-antibody-271120242022971.jpg)
![Immunohistochemistry: DFF40/CAD Antibody - BSA Free [NBP1-77009] - Immunohistochemistry of DFF40/CAD in mouse kidney tissue with DFF40/CAD antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77009_rabbit-polyclonal-dff40-cad-antibody-271120242045619.jpg "Immunohistochemistry: DFF40/CAD Antibody - BSA Free [NBP1-77009] - Immunohistochemistry of DFF40/CAD in mouse kidney tissue with DFF40/CAD antibody at 2 ug/mL.")
![Western Blot: DFF40/CAD Antibody [NBP1-77009] - Analysis of 50 ug of murine lung tissue lysate with anti-CAD (I17) at 1:500 dilution.](http://images.novusbio.com/fullsize/DFF40-CAD-Antibody-Western-Blot-NBP1-77009-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: DFF40/CAD Antibody [NBP1-77009] - Staining of CAD in mouse kidney tissue with CAD antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/DFF40-CAD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77009-img0004.jpg)
![Immunohistochemistry: DFF40/CAD Antibody - BSA Free [NBP1-77009] - Immunohistochemistry of DFF40/CAD in mouse kidney tissue with DFF40/CAD antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp1-77009_rabbit-polyclonal-dff40-cad-antibody-271120242045637.gif)
![Immunocytochemistry/ Immunofluorescence: DFF40/CAD Antibody - BSA Free [NBP1-77009] - Immunofluorescence of DFF40/CAD in Mouse Kidney cells with DFF40/CAD antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77009_rabbit-polyclonal-dff40-cad-antibody-2711202419313271.jpg)
![Western Blot: CRTH-2/GPR44 Antibody [NBP1-76755] - Jurkat cell lysate with GPR44 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/CRTH-2-GPR44-Antibody-Western-Blot-NBP1-76755-img0003.jpg "Western Blot: CRTH-2/GPR44 Antibody [NBP1-76755] - Jurkat cell lysate with GPR44 antibody at 1 ug/ml in (A) the absence and (B) presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: CRTH-2/GPR44 Antibody - BSA Free [NBP1-76755] - Immunofluorescence of CRTH-2/GPR44 in Human Heart tissue with CRTH-2/GPR44 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76755_rabbit-polyclonal-crth-2-gpr44-antibody-271120241932638.jpg)
![Immunohistochemistry: CRTH-2/GPR44 Antibody - BSA Free [NBP1-76755] - Immunohistochemistry of CRTH-2/GPR44 in human heart tissue with CRTH-2/GPR44 antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76755_rabbit-polyclonal-crth-2-gpr44-antibody-2711202420333858.jpg)
![Western Blot: ARMER Antibody [NBP1-77198] - Analysis of ARMER in human bladder cell lysates with anti-ARMER (CT) using NBP1-77198 at (A) 0.5, (B) 1, and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/ARMER-Antibody-Western-Blot-NBP1-77198-img0004.jpg "Western Blot: ARMER Antibody [NBP1-77198] - Analysis of ARMER in human bladder cell lysates with anti-ARMER (CT) using NBP1-77198 at (A) 0.5, (B) 1, and (C) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: ARMER Antibody - BSA Free [NBP1-77198] - Immunofluorescence of ARMER in Mouse Intestine cells with ARMER antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77198_rabbit-polyclonal-armer-antibody-2711202419381777.jpg)
![Immunohistochemistry: ARMER Antibody - BSA Free [NBP1-77198] - Immunohistochemical staining of human bladder tissue using ARMER antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77198_rabbit-polyclonal-armer-antibody-2711202420392797.jpg)
![Western Blot: MICA Antibody [NBP1-76805] - A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.](http://images.novusbio.com/fullsize/MICA-Antibody-Western-Blot-NBP1-76805-img0001.jpg "Western Blot: MICA Antibody [NBP1-76805] - A-20 cell lysate with MICA antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.")
![Immunocytochemistry/Immunofluorescence: MICA Antibody [NBP1-76805] - analysis of MICA in Jukat cells using anti-MICA antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/MICA-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76805-img0004.jpg)
![Immunohistochemistry-Paraffin: MICA Antibody [NBP1-76805] - Human colon tissue with MICA antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/MICA-Antibody-Immunohistochemistry-Paraffin-NBP1-76805-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: MICA Antibody - BSA Free [NBP1-76805] - Immunofluorescence of MICA in Human Colon tissue with MICA antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76805_rabbit-polyclonal-mica-antibody-271120242019539.jpg)