Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7530APC | |||
| Biotin | NB7530B | |||
| DyLight 350 | NB7530UV | |||
| DyLight 405 | NB7530V | |||
| DyLight 488 | NB7530G | |||
| DyLight 550 | NB7530R | |||
| DyLight 594 | NB7530DL594 | |||
| DyLight 650 | NB7530C | |||
| DyLight 680 | NB7530FR | |||
| DyLight 755 | NB7530IR | |||
| FITC | NB7532 | |||
| HRP | NB7533 | |||
| Janelia Fluor 525 | NB7530JF525 | |||
| Janelia Fluor 669 | NB7530JF669 | |||
| PE | NB7530PE | |||
| PerCP | NB7530PCP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB9435U-100UG | |||
| Alexa Fluor 405 | FAB9435V-100UG | |||
| Alexa Fluor 488 | FAB9435G-100UG | |||
| Alexa Fluor 532 | FAB9435X-100UG | |||
| Alexa Fluor 594 | FAB9435T-100UG | |||
| Alexa Fluor 647 | FAB9435R-100UG | |||
| Alexa Fluor 700 | FAB9435N-100UG | |||
| Alexa Fluor 750 | FAB9435S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9435-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51582SS | |||
| Azide and BSA Free | NBP2-80720 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7546APC | |||
| Biotin | NB7546B | |||
| DyLight 350 | NB7546UV | |||
| DyLight 405 | NB7546V | |||
| DyLight 488 | NB7546G | |||
| DyLight 550 | NB7546R | |||
| DyLight 594 | NB7546DL594 | |||
| DyLight 650 | NB7546C | |||
| DyLight 680 | NB7546FR | |||
| DyLight 755 | NB7546IR | |||
| FITC | NB7548 | |||
| HRP | NB7549 | |||
| Janelia Fluor 525 | NB7546JF525 | |||
| Janelia Fluor 669 | NB7546JF669 | |||
| PE | NB7546PE | |||
| PerCP | NB7546PCP | |||
![N/A IL-12/IL-23 p40 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL12_p40_DY1969_ELISA_1741.jpg "N/A IL-12/IL-23 p40 [Biotin]")
![N/A IL-17A/F Heterodimer [HRP]](http://images.novusbio.com/fullsize2/DATA_IL17A_F_Heterodimer_M17AF0_ELISA_925.jpg "N/A IL-17A/F Heterodimer [HRP]")
![N/A IL-17A/F Heterodimer [HRP]](http://images.novusbio.com/fullsize2/IL-17A_F_Heterodimer_M17AF0_ELISA_228.jpg)
![N/A Reg3A [Biotin]](http://images.novusbio.com/fullsize2/DATA_Reg3A_DY594005_ELISA_2483.jpg "N/A Reg3A [Biotin]")
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0015.jpg "Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Lane M: Super Signal Molecular Weight Marker.Lane 1: HeLa WCL. Load: 30 ug lysate.Primary antibody: ROBO1 antibody at 1:1,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 181 kDa, 181 kDa for ROBO1. Other band(s): lower bands not identified.")
![Immunocytochemistry/Immunofluorescence: ROBO1 Antibody [NB600-1254] - Staining of Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with primary Ab for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunocytochemistry-Immunofluorescence-NB600-1254-img0005.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - Affinity Purified ROBO1 antibody was used at a concentration of 5 ug/ml to detect ROBO1 in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows staining of human brain tissue. Tissue was formalin-fixed and paraffin embedded.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0016.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Biotinylated human mTOR present in a 293T whole cell lysates. Cells were serum-starved for 24 hours prior to harvest. ~20ug of lysate was loaded per lane for SDS-PAGE. Untreated cells are shown in lane 1, whereas cells in lane 2 were treated with IGF-1 (100 ng/ml) for 20 min prior to harvest. Follow reaction of antibody with a 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0012.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western blot using Affinity Purified ROBO1 antibody shows detection of a band at ~181 kDa corresponding to ROBO1 present in mouse brain lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-8% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,000. Reaction occurred 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0013.jpg)
![Western Blot: ROBO1 Antibody [NB600-1254] - Western Blot of ROBO1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate. Lane 3: MCF-7 Lysate. Lane 4: Jurkat Lysate. Lane 5: A431 Lysate. Lane 6: A549 Lysate. Lane 7: LNCap Lysate. Lane 8: MOLT-4 Lysate. Lane 9: Ramos Lysate. Lane 10: Raji Lysate. Lane 11: A-172 Lysate. Lane 12: NIH/3T3 Lysate. Load: 35 ug per lane. Primary antibody: ROBO1 antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted/Observed size: 181kDa for ROBO1.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Western-Blot-NB600-1254-img0014.jpg)
![Immunohistochemistry: ROBO1 Antibody [NB600-1254] - 1/50 staining mouse lung tissue sections (adult, frozen 100um wholemount sections) by IHC-Fr. The tissue was paraformaldehyde fixed and permeabilized with triton x-100 before incubation with the antibody for 16 hours at 4C.](http://images.novusbio.com/fullsize/ROBO1-Antibody-Immunohistochemistry-NB600-1254-img0011.jpg)
![ELISA: ROBO1 Antibody [NB600-1254] - ELISA results of purified ROBO1 antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/ROBO1-Antibody-ELISA-NB600-1254-img0017.jpg)
![N/A alpha 1-Acid Glycoprotein [HRP]](http://images.novusbio.com/fullsize2/DATA_alpha_1Acid_Glycoprotein_DAGP00_ELISA_568.jpg "N/A alpha 1-Acid Glycoprotein [HRP]")
![N/A alpha 1-Acid Glycoprotein [HRP]](http://images.novusbio.com/fullsize2/alpha_1-Acid_Glycoprotein_DAGP00_ELISA_26.jpg)
![N/A PBEF/Visfatin/NAMPT [Biotin]](http://images.novusbio.com/fullsize2/DATA_PBEF_DY433505_ELISA_2455.jpg "N/A PBEF/Visfatin/NAMPT [Biotin]")
![Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Western-Blot-NBP1-51582-img0003.jpg "Western Blot: eNOS Antibody (6H2) [NBP1-51582] - Western blot analysis of HUVEC cells (A) using eNOS antibody at a concentration of 2 ug/ml.")
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - IHC staining of eNOS in human placenta using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0004.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human lymph node (left) and colon cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0001.jpg)
![Immunohistochemistry-Paraffin: eNOS Antibody (6H2) [NBP1-51582] - Immunohistochemical analysis of paraffin-embedded human stomach cancer (left) and ovary cancer (right) tissues using eNOS mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eNOS-Antibody-6H2-Immunohistochemistry-Paraffin-NBP1-51582-img0002.jpg)
![N/A Complement Factor D/Adipsin [HRP]](http://images.novusbio.com/fullsize2/DATA_Complement_Factor_D_DFD00_ELISA_671.jpg "N/A Complement Factor D/Adipsin [HRP]")
![N/A Complement Factor D/Adipsin [HRP]](http://images.novusbio.com/fullsize2/Complement_Factor_D_DFD00_ELISA_92.jpg)
![N/A IL-17/IL-17A [Biotin]](http://images.novusbio.com/fullsize2/DATA_IL17_DY5848_ELISA_2138.jpg "N/A IL-17/IL-17A [Biotin]")
![Western Blot: Goat anti- Mouse, Human Kappa Light Chain Secondary Antibody [NB7546] - Analysis in 1. c whole cell lysate, 2. ASPC1 tumor lysate. Image from verified customer review. Image using the HRP format of this antibody.](http://images.novusbio.com/fullsize/Goat-anti--Mouse--Human-Kappa-Light-Chain-Secondary-Antibody-Western-Blot-NB7546-img0001.jpg "Western Blot: Goat anti- Mouse, Human Kappa Light Chain Secondary Antibody [NB7546] - Analysis in 1. c whole cell lysate, 2. ASPC1 tumor lysate. Image from verified customer review. Image using the HRP format of this antibody.")