Goat anti-Mouse IgE Heavy Chain Secondary Antibody

• Reactivity: Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Goat anti-Mouse IgE Heavy Chain Secondary Antibody Summary

• Immunogen: Mouse IgE
• Specificity: By immunoelectrophoresis and ELISA this reacts specifically with mouse IgE. This may cross react with IgE from other species.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000 - 1:30000
  • Immunocytochemistry/ Immunofluorescence 1:200- 1:2000
  • Immunohistochemistry 1:200- 1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000 - 1:30000
• Application Notes: ELISA 1:1,000 - 1:30,000; for coating plates 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate Buffered Saline (PBS)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Goat anti-Mouse IgE Heavy Chain Secondary Antibody

• Constant Region of Heavy Chain of IgE
• Ig Epsilon Chain C Region ND
• Ig Epsilon Chain C Region
• IgE
• Immunoglobulin Epsilon
• immunoglobulin heavy constant epsilon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgE Antibody (NB7530)(9)

Publications using NB7530

• Palaniyandi S, Liu X, Periasamy S et al. Inhibition of CD23-mediated IgE transcytosis suppresses the initiation and development of allergic airway inflammation. Mucosal Immunol. [PMID: 25783969]
  
  Details:
  Citation using the FITC form of this antibody.
• Wang L, Gao S, Xu W et al. Allergic asthma accelerates atherosclerosis dependent on Th2 and Th17 in apolipoprotein E deficient mice J Mol Cell Cardiol 2014-02-18 [PMID: 24530901] (IHC-Fr)
• Vaickus LJ, Bouchard J, Kim J et al. Cockroach allergens induce biphasic asthma-like pulmonary inflammation in outbred mice. J Asthma. [PMID: 22540923]
  
  Details:
  Citation using the HRP form of this antibody.
• Damayanti T, Kikuchi T, Zaini J et al. Serial OX40 engagement on CD4+ T cells and natural killer T cells causes allergic airway inflammation. Am J Respir Crit Care Med . [PMID: 20019337]
  
  Details:
  Citation using the HRP form of this antibody.
• Sakai S, Sugawara T, Matsubara K et al. Inhibitory effect of carotenoids on the degranulation of mast cells via suppression of antigen-induced aggregation of high affinity IgE receptors. J Biol Chem 2009-10-01 [PMID: 19700409]
• Polte T, Foell J, Werner C et al. CD137-mediated immunotherapy for allergic asthma. J Clin Invest. [PMID: 16528411]
  
  Details:
  Citation using the HRP form of this antibody.
• Sejima T, Madoiwa S, Mimuro J et al. Protection of plasminogen activator inhibitor-1-deficient mice from nasal allergy. J Immunol. [PMID: 15944322]
  
  Details:
  Citation using the HRP form of this antibody.
• Neel NF, Creasy BM, Rankin JN et al. Absence of interleukin-3 does not affect the severity of local and systemic anaphylaxis but does enhance eosinophil infiltration in a mouse model of allergic peritonitis. Immunol Lett 2004-08-01 [PMID: 15325796]
• Voice JK, Dorsam G, Lee H et al. Allergic diathesis in transgenic mice with constitutive T cell expression of inducible vasoactive intestinal peptide receptor. FASEB J. [PMID: 11689474]
  
  Details:
  Citation using the HRP form of this antibody.