452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP-2 Monoclonal Antibody (Catalog # MAB452). The ND₅₀ is typically 1-4 µg/mL." class="big_lightbox"> 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP-2 Monoclonal Antibody (Catalog # MAB452). The ND₅₀ is typically 1-4 µg/mL." alt="Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL2/MIP-2 Monoclonal Antibody (Catalog # MAB452). The ND₅₀ is typically 1-4 µg/mL." class="big_thumb" />

452-M2) was serially diluted and captured by Rat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Monoclonal Antibody (Catalog # MAB452) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 452-M2) was serially diluted and captured by Rat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Monoclonal Antibody (Catalog # MAB452) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CXCL2/GRO beta /MIP‑2/CINC‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-452-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />