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![Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-28820241246162.jpg "Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-29202410234222.jpg)
![Immunohistochemistry-Paraffin: Wnt-10a Antibody [NBP3-14379] - Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreatic islet after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Wnt-10a-Antibody-Immunohistochemistry-Paraffin-NBP3-14379-img0003.jpg "Immunohistochemistry-Paraffin: Wnt-10a Antibody [NBP3-14379] - Immunohistochemistry of formalin-fixed, paraffin-embedded human pancreatic islet after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Wnt-10a Antibody [NBP3-14379] - Immunohistochemistry of formalin-fixed, paraffin-embedded human prostate](http://images.novusbio.com/fullsize/Wnt-10a-Antibody-Immunohistochemistry-Paraffin-NBP3-14379-img0001.jpg)
![Immunohistochemistry-Paraffin: Wnt-10a Antibody [NBP3-14379] - Immunohistochemistry of formalin-fixed, paraffin-embedded human stomach](http://images.novusbio.com/fullsize/Wnt-10a-Antibody-Immunohistochemistry-Paraffin-NBP3-14379-img0002.jpg)
![Immunohistochemistry-Paraffin: Wnt-10a Antibody [NBP3-14379] - Immunohistochemistry of formalin-fixed, paraffin-embedded human lymph node, melanoma,](http://images.novusbio.com/fullsize/Wnt-10a Antibody-Immunohistochemistry-Paraffin-NBP3-14379-img0004.jpg)
![Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Western-Blot-NBP2-99335-img0004.jpg "Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa")
![Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99335-img0003.jpg)
![Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99335-img0002.jpg)
![Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-288202412442968.jpg "Western Blot: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: Human brain tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32846) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Immunocytochemistry analysis of A431 cells labeling PPP2R5D with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PPP2R5D antibody (NBP3-32846) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-2920241192344.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-21) [NBP3-32846] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32846, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32846_rabbit-ppp2r5d-mab-psh01-21-29202416372164.jpg)
![Western Blot: PKC alpha Antibody (G13-R) [NBP1-30121] - Lane 1-50 ng; lane 2-100 ng; lane 3 -200 ng of recombinant human.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-G13-R-Western-Blot-NBP1-30121-img0003.jpg "Western Blot: PKC alpha Antibody (G13-R) [NBP1-30121] - Lane 1-50 ng; lane 2-100 ng; lane 3 -200 ng of recombinant human.")
![Immunocytochemistry/Immunofluorescence: PKC alpha Antibody (G13-R) [NBP1-30121] - Expression in HEK293 cells, visualized with dilution - 1:100.](http://images.novusbio.com/fullsize/PKC-alpha-Antibody-G13-R-Immunocytochemistry-Immunofluorescence-NBP1-30121-img0004.jpg)
![Western Blot: PKC gamma Antibody (A12-H) [NBP1-30124] - Left Lane: Marker, Right Lane: Mouse brain crude lysate (200ug of protein loaded).](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-A12-H-Western-Blot-NBP1-30124-img0001.jpg "Western Blot: PKC gamma Antibody (A12-H) [NBP1-30124] - Left Lane: Marker, Right Lane: Mouse brain crude lysate (200ug of protein loaded).")
![Immunocytochemistry/Immunofluorescence: PKC gamma Antibody (A12-H) [NBP1-30124] - PKC-gamma expression in HEK293 cells.](http://images.novusbio.com/fullsize/PKC-gamma-Antibody-A12-H-Immunocytochemistry-Immunofluorescence-NBP1-30124-img0003.jpg)
![Western Blot: PKC alpha Antibody (M237) [NBP3-23297] - Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKC alpha antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKC alpha (Central region) or anti-phospho-PKC alpha (Ser-657/Tyr-658). The latter shows no detection of PKC alpha after phosphatase treatment.](http://images.novusbio.com/fullsize/nbp3-23297_mouse-pkc-alpha-mab-m237-1992023191549.jpg "Western Blot: PKC alpha Antibody (M237) [NBP3-23297] - Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKC alpha antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKC alpha (Central region) or anti-phospho-PKC alpha (Ser-657/Tyr-658). The latter shows no detection of PKC alpha after phosphatase treatment.")
![Immunocytochemistry/Immunofluorescence: PKC alpha [p Ser657, p Tyr658] Antibody [NBP3-23303] - Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKC alpha (Ser-657/Tyr-658) (NBP3-23303) antibody in the absence (Left) or presence (Right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23303_rabbit-pkc-alpha-p-ser657-p-tyr658-pab-19920231759448.jpg "Immunocytochemistry/Immunofluorescence: PKC alpha [p Ser657, p Tyr658] Antibody [NBP3-23303] - Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKC alpha (Ser-657/Tyr-658) (NBP3-23303) antibody in the absence (Left) or presence (Right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: PKC alpha [p Ser657, p Tyr658] Antibody [NBP3-23303] - Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKC alpha antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKC alpha (Central region) or anti-phospho-PKC alpha (Ser-657/Tyr-658). The latter shows no detection of PKC alpha after phosphatase treatment.](http://images.novusbio.com/fullsize/nbp3-23303_rabbit-pkc-alpha-p-ser657-p-tyr658-pab-19920231856932.jpg)
![Western Blot: Wnt-3a Antibody (C2) [NBP3-42133] - Sample: MCF7 cell lysate Primary Ab: 3ug/ml NBP3-42133; Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42133_mouse-wnt-3a-mab-c2-61220242151864.jpg "Western Blot: Wnt-3a Antibody (C2) [NBP3-42133] - Sample: MCF7 cell lysate Primary Ab: 3ug/ml NBP3-42133; Second Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Wnt-3a Antibody (C2) [NBP3-42133] - DAB staining on IHC-P; Sample: Human Small intestine Tissue; Primary Ab: 30ug/ml NBP3-42133; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42133_mouse-wnt-3a-mab-c2-612202421395343.jpg)
![Western Blot: Wnt-3a Antibody (C2) [NBP3-42133] - Sample: Recombinant Wnt-3a, Human.](http://images.novusbio.com/fullsize/nbp3-42133_mouse-wnt-3a-mab-c2-61220242116236.jpg)
![Immunohistochemistry-Paraffin: Wnt-4 Antibody (C1) [NBP3-42123] - DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10ug/ml NBP3-42123; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42123_mouse-wnt-4-mab-c1-6122024211621.jpg "Immunohistochemistry-Paraffin: Wnt-4 Antibody (C1) [NBP3-42123] - DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 10ug/ml NBP3-42123; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: Wnt-4 Antibody (C1) [NBP3-42123] - DAB staining on IHC-P; Samples: Human Cardiac Muscle Tissue; Primary Ab: 10ug/ml NBP3-42123; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42123_mouse-wnt-4-mab-c1-612202421195697.jpg)
![Western Blot: Wnt-4 Antibody (C1) [NBP3-42123] - Sample: Recombinant Wnt-4, Human.](http://images.novusbio.com/fullsize/nbp3-42123_mouse-wnt-4-mab-c1-6122024217133.jpg)
![Western Blot: ROCK2 Antibody (10E1) [NBP3-15152] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: ROCK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 161 KDa. Observed band size: 161 KDa.](http://images.novusbio.com/fullsize/ROCK2-Antibody-10E1-Western-Blot-NBP3-15152-img0002.jpg "Western Blot: ROCK2 Antibody (10E1) [NBP3-15152] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, HepG2 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: ROCK2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 161 KDa. Observed band size: 161 KDa.")
![Immunocytochemistry/Immunofluorescence: ROCK2 Antibody (10E1) [NBP3-15152] - Immunofluorescence staining of Hela Cells with at ROCK2 antibody 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/ROCK2-Antibody-10E1-Immunocytochemistry-Immunofluorescence-NBP3-15152-img0001.jpg)