| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38201-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37510-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98812-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99686-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99335-50ul | |||
![Western Blot: MMP-7 Antibody (9D8S10) [NBP3-16067] - Analysis of extracts of various cell lines, using MMP-7 antibody (NBP3-16067 ) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/MMP-7-Antibody-9D8S10-Western-Blot-NBP3-16067-img0005.jpg "Western Blot: MMP-7 Antibody (9D8S10) [NBP3-16067] - Analysis of extracts of various cell lines, using MMP-7 antibody (NBP3-16067 ) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry: MMP-7 Antibody (8T4R8) [NBP3-16067] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using MMP-7 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16067_rabbit-mmp-7-mab-8t4r8-52202511374122.jpg)
![Western Blot: PKC beta Antibody [NBP3-38201] - Western blot analysis of various lysates using PKC beta Rabbit pAb at 1:3000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38201_rabbit-pkc-beta-pab-912202414112810.jpg "Western Blot: PKC beta Antibody [NBP3-38201] - Western blot analysis of various lysates using PKC beta Rabbit pAb at 1:3000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: PKC beta Antibody [NBP3-38201] - Immunofluorescence analysis of NIH/3T3 cells using PKC beta Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38201_rabbit-pkc-beta-pab-3112025177029.jpg)
![Western Blot: Wnt-5a Antibody (3P2E8) [NBP3-15684] - Western blot analysis of extracts of various cell lines, using Wnt-5a antibody (NBP3-15684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Wnt-5a-Antibody-3P2E8-Western-Blot-NBP3-15684-img0002.jpg "Western Blot: Wnt-5a Antibody (3P2E8) [NBP3-15684] - Western blot analysis of extracts of various cell lines, using Wnt-5a antibody (NBP3-15684) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Wnt-5a Antibody (3P2E8) [NBP3-15684] - Immunohistochemistry of paraffin-embedded human lung cancer using Wnt-5a Rabbit mAb (NBP3-15684) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Wnt-5a-Antibody-3P2E8-Immunohistochemistry-Paraffin-NBP3-15684-img0001.jpg)
![Western Blot: LRP-5 Antibody (2B11) [NBP2-37510] - Western blot analysis using LRP5 mAb against human LRP5 (AA: 1422-1615) recombinant protein. (Expected MW is 20.8 kDa)](http://images.novusbio.com/fullsize/LRP-5-Antibody-2B11-Western-Blot-NBP2-37510-img0002.jpg "Western Blot: LRP-5 Antibody (2B11) [NBP2-37510] - Western blot analysis using LRP5 mAb against human LRP5 (AA: 1422-1615) recombinant protein. (Expected MW is 20.8 kDa)")
![Flow Cytometry: LRP-5 Antibody (2B11) [NBP2-37510] - Flow cytometric analysis of HeLa cells using LRP5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/LRP-5-Antibody-2B11-Flow-Cytometry-NBP2-37510-img0003.jpg)
![ELISA: LRP-5 Antibody (2B11) [NBP2-37510] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/LRP-5-Antibody-2B11-ELISA-NBP2-37510-img0001.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody [NBP2-98812] - Immunochemical staining of human JNK2 in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunohistochemistry-Paraffin-NBP2-98812-img0003.jpg "Immunohistochemistry-Paraffin: JNK2 Antibody [NBP2-98812] - Immunochemical staining of human JNK2 in human pancreas with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).")
![Immunohistochemistry-Paraffin: JNK2 Antibody [NBP2-98812] - Immunochemical staining of human JNK2 in human lung cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunohistochemistry-Paraffin-NBP2-98812-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody [NBP2-98812] - Immunofluorescence staining of JNK2 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98812 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98812_rabbit-polyclonal-jnk2-antibody-235202511545667.png)
![Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-28820241246162.jpg "Western Blot: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Western blot analysis of SIAH1 on different lysates with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate(20 ug/Lane) Lane 2: RAW264.7 cell lysate(20 ug/Lane) Lane 3: Mouse liver tissue lysate(40 ug/Lane) Lane 4: Mouse brain tissue lysate(40 ug/Lane) Lane 5: Rat liver tissue lysate(40 ug/Lane) Lane 6: Rat brain tissue lysate(40 ug/Lane) Lane 7: Rat testis testis lysate(40 ug/Lane) Predicted band size: 31 kDa Observed band size: 25 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32949) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: SIAH1 Antibody (PSH01-80) [NBP3-32949] - Immunocytochemistry analysis of A549 cells labeling SIAH1 with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SIAH1 antibody (NBP3-32949) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32949_rabbit-siah1-mab-psh01-80-29202410234222.jpg)
![Western Blot: PPP2R5A Antibody [NBP3-30773] - Legend: Novus Biologicals' rabbit anti PP 2 A/B alpha western blot of total rat brain homogenate.(+) indicates presence of specific blocking peptide of control peptide (-).](http://images.novusbio.com/fullsize/nbp3-30773_rabbit-ppp2r5a-pab-811202420592192.jpg "Western Blot: PPP2R5A Antibody [NBP3-30773] - Legend: Novus Biologicals' rabbit anti PP 2 A/B alpha western blot of total rat brain homogenate.(+) indicates presence of specific blocking peptide of control peptide (-).")
![Western Blot: CKII alpha prime polypeptide Antibody [NBP2-99686] - Anti-CKII alpha prime polypeptide rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Western-Blot-NBP2-99686-img0005.jpg "Western Blot: CKII alpha prime polypeptide Antibody [NBP2-99686] - Anti-CKII alpha prime polypeptide rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 45 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: CKII alpha prime polypeptide Antibody [NBP2-99686] - CKII alpha prime polypeptide was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate4 ul anti-CKII alpha prime polypeptide rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CKII alpha prime polypeptide rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 41 kDa. Observed band size: 41 kDa](http://images.novusbio.com/fullsize/CKII-alpha-prime-polypeptide-Antibody-Immunoprecipitation-NBP2-99686-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP2-99686] - Immunofluorescence staining of CKII alpha prime polypeptide in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-99686 antibody (1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-99686_rabbit-polyclonal-ckii-alpha-prime-polypeptide-antibody-23520251664765.png)
![Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Western-Blot-NBP2-99335-img0004.jpg "Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa")
![Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99335-img0003.jpg)
![Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99335-img0002.jpg)
![Western Blot: PPP2R5A Antibody [NBP3-30774] - Legend: Novus Biologicals' sheep anti PP 2 A/B alpha western blot of total rat brain homogenate.](http://images.novusbio.com/fullsize/nbp3-30774_sheep-ppp2r5a-pab-81120242059218.jpg "Western Blot: PPP2R5A Antibody [NBP3-30774] - Legend: Novus Biologicals' sheep anti PP 2 A/B alpha western blot of total rat brain homogenate.")
![Immunohistochemistry: PPP2R1B Antibody (PSH02-92) [NBP3-32845] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PPP2R1B antibody (NBP3-32845) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32845) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32845_rabbit-ppp2r1b-mab-psh02-92-29820241435296.jpg "Immunohistochemistry: PPP2R1B Antibody (PSH02-92) [NBP3-32845] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-PPP2R1B antibody (NBP3-32845) at 1/200 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32845) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32845 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lane 5: RAW264.7 cell lysate Lane 6: Mouse testis tissue lysate Lane 7: Rat testis tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32845 at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32845_-western-blot-639204597144499147.jpg)