| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97476-0.2mg | |||
| BSA Free | NBP1-97476-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54552-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37279-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59692-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54318-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88023-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26497-50ul | |||
![Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-97476-img0002.jpg "Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97476] - Analysis of human placenta tissue stained with Calnexin, pAb at 5ug/ml.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-97476-img0001.jpg)
![Western Blot: Calnexin Antibody (CANX/1541) [NBP2-53352] - Western Blot Analysis of HeLa and MCF-7 cell lysate using Calnexin Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Western-Blot-NBP2-53352-img0007.jpg "Western Blot: Calnexin Antibody (CANX/1541) [NBP2-53352] - Western Blot Analysis of HeLa and MCF-7 cell lysate using Calnexin Antibody (CANX/1541).")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (CANX/1541) [NBP2-53352] - Confocal immunofluorescence image of HeLa cells using Calnexin Antibody (CANX/1541), labeled in Green. Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Immunocytochemistry-Immunofluorescence-NBP2-53352-img0005.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1541) [NBP2-53352] - Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Immunohistochemistry-Paraffin-NBP2-53352-img0003.jpg)
![Western Blot: Calnexin Antibody (CANX/1541) [NBP2-53352] - Western Blot Analysis of Human Kidney lysate using Calnexin Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Western-Blot-NBP2-53352-img0006.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1541) [NBP2-53352] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Immunohistochemistry-Paraffin-NBP2-53352-img0001.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1541) [NBP2-53352] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Immunohistochemistry-Paraffin-NBP2-53352-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody diluted at a dilution of 1:10000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-42848-img0009.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody diluted at a dilution of 1:10000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-42848] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Hspa5 protein stained by Hspa5 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-42848-img0006.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Paraffin-embedded rat brain. Hspa5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-42848-img0008.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of 30 ug whole cell lysate. A: NIH-3T-3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 7.5% SDS PAGE diluted at 1:10000..](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-42848-img0003.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of A. 50 ug Rat heart lysate/extract B. 50 ug Rat Lung lysate/extract C. 50 ug Rat Liver lysate/extract D. 50 ug Rat kidney lysate/extract 7.5 % SDS-PAGE Hspa5 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-42848-img0004.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of gastric carcinoma. Hspa5 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-42848-img0001.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Paraffin-embedded mouse liver. Hspa5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-42848-img0007.jpg)
![Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mouse mAb against MCF-7 (1), PC-12 (2), HepG2 (3), Hela (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Western-Blot-NBP2-37279-img0004.jpg "Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mouse mAb against MCF-7 (1), PC-12 (2), HepG2 (3), Hela (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7A8) [NBP2-37279] - Immunofluorescence analysis of HepG2 cells using EIF2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Immunocytochemistry-Immunofluorescence-NBP2-37279-img0005.jpg)
![Immunohistochemistry: eIF2A Antibody (3A7A8) [NBP2-37279] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EIF2A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Immunohistochemistry-NBP2-37279-img0007.jpg)
![Flow Cytometry: eIF2A Antibody (3A7A8) [NBP2-37279] - Flow cytometric analysis of HepG2 cells using EIF2A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Flow-Cytometry-NBP2-37279-img0006.jpg)
![Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mAb against human EIF2A (AA: 448-576) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Western-Blot-NBP2-37279-img0002.jpg)
![Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mAb against HEK293 (1) and EIF2A (AA: 448-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Western-Blot-NBP2-37279-img0003.jpg)
![ELISA: eIF2A Antibody (3A7A8) [NBP2-37279] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-ELISA-NBP2-37279-img0001.jpg)
![Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Western-Blot-NBP2-66856-img0007.jpg "Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0006.jpg)
![Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Flow-Cytometry-NBP2-66856-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0005.jpg)
![Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Western-Blot-NBP2-59692-img0002.jpg "Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Mouse fibroblast cell line (NIH 3T3). Fixed in 4% Formaldehyde for 15 min at RT. Primary antibody at 1:100 for 60 min at RT. Secondary antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstaied with DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Immunocytochemistry-Immunofluorescence-NBP2-59692-img0003.jpg)
![Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0002.jpg "Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Western Blot: XBP1 Antibody [NBP2-20917] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N1C3] diluted at 1:1000.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0001.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-54318-img0008.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-54318] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54318-img0006.jpg)
![Western Blot: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Western blot analysis of eIF2 alpha/EIF2S1 on different lysates with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: COS-1 cell lysate Lane 5: A549 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat kidney tissue lysate Lane 11: Rat spleen tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32296) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-27820241628526.jpg "Western Blot: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Western blot analysis of eIF2 alpha/EIF2S1 on different lysates with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/2,000 dilution. Lane 1: MCF7 cell lysate Lane 2: HepG2 cell lysate Lane 3: HeLa cell lysate Lane 4: COS-1 cell lysate Lane 5: A549 cell lysate Lane 6: RAW264.7 cell lysate Lane 7: C6 cell lysate Lane 8: Mouse kidney tissue lysate Lane 9: Mouse spleen tissue lysate Lane 10: Rat kidney tissue lysate Lane 11: Rat spleen tissue lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32296) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32296) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-29820241420926.jpg)
![Immunocytochemistry/ Immunofluorescence: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Immunocytochemistry analysis of HepG2 cells labeling eIF2 alpha/EIF2S1 with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF2 alpha/EIF2S1 antibody (NBP3-32296) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-29202410484850.jpg)
![Flow Cytometry: eIF2 alpha/EIF2S1 Antibody (PSH04-29) [NBP3-32296] - Flow cytometric analysis of HepG2 cells labeling eIF2 alpha/EIF2S1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32296, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32296_rabbit-eif2-alpha-eif2s1-mab-psh04-29-2920241645323.jpg)
![Western Blot: SKIV2L Antibody [NBP2-47274] - Analysis in control (vector only transfected HEK293T lysate) and SKIV2L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SKIV2L-Antibody-Western-Blot-NBP2-47274-img0006.jpg "Western Blot: SKIV2L Antibody [NBP2-47274] - Analysis in control (vector only transfected HEK293T lysate) and SKIV2L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: SKIV2L Antibody [NBP2-47274] - Staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SKIV2L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47274-img0005.jpg)
![Immunohistochemistry-Paraffin: SKIV2L Antibody [NBP2-47274] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/SKIV2L-Antibody-Immunohistochemistry-Paraffin-NBP2-47274-img0004.jpg)
![Western Blot: Derlin 1 Antibody [NBP1-88023] - Analysis in human cell line MCF-7.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Western-Blot-NBP1-88023-img0008.jpg "Western Blot: Derlin 1 Antibody [NBP1-88023] - Analysis in human cell line MCF-7.")
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NBP1-88023] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88023-img0009.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0013.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0010.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0011.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in lymphoid cells and in glandular cells.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0012.jpg)
![Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241322155.jpg "Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: ATF4 Antibody (9E1) [NBP3-26497] - Image of ATF4 Antibody (9E1) diluted at 1:160 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241510616.jpg)
![Western Blot: ATF4 Antibody (9E1) [NBP3-26497] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: ATF4 Antibody at 1.6ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 KDa Observed band size: 50 KDa](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202411472134.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (9E1) [NBP3-26497] - Staining of HepG2 cells with ATF4 Antibody (9E1) at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-28220241312211.jpg)
![Flow Cytometry: ATF4 Antibody (9E1) [NBP3-26497] - Overlay histogram showing Hela cells stained with ATF4 Antibody (9E1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202414312786.jpg)