| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79835 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12888-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84879-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85520-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26427-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42176-0.025mg | |||
![Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of various cell lines, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Western-Blot-NBP3-16864-img0004.jpg "Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of various cell lines, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] - Immunohistochemistry of paraffin-embedded mouse testis using eIF2A Rabbit mAb (NBP3-16864) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Immunohistochemistry-Paraffin-NBP3-16864-img0003.jpg)
![Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of Rat lung, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Western-Blot-NBP3-16864-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] - Immunohistochemistry of paraffin-embedded rat pancreas using eIF2A Rabbit mAb (NBP3-16864) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Immunohistochemistry-Paraffin-NBP3-16864-img0001.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] -Analysis of paraffin-embedded Human tonsil tissue using eIF2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16864_rabbit-eif2a-mab-8v8h7-immunohistochemistry-paraffin-73202594938..png)
![Western Blot: Calnexin Antibody (CANX/1543) [NBP2-79701] - Western Blot of Analysis of human Kidney lysate using Calnexin Antibody (CANX/1543).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Western-Blot-NBP2-79701-img0004.jpg "Western Blot: Calnexin Antibody (CANX/1543) [NBP2-79701] - Western Blot of Analysis of human Kidney lysate using Calnexin Antibody (CANX/1543).")
![Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1543) [NBP2-79701] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Antibody (CANX/1543).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Immunohistochemistry-Paraffin-NBP2-79701-img0001.jpg)
![Western Blot: Calnexin Antibody (CANX/1543) [NBP2-79701] - Western Blot of Analysis of PANC1 and MCF-7 cell lysate using Calnexin Antibody (CANX/1543).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Western-Blot-NBP2-79701-img0003.jpg)
![Protein Array: Calnexin Antibody (CANX/1543) [NBP2-79701] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Calnexin Antibody (CANX/1543). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1543-Protein-Array-NBP2-79701-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Western-Blot-NBP2-12888-img0003.jpg "Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0005.jpg)
![Western Blot: eIF2A Antibody [NBP1-84879] - Analysis in human cell line RPMI-8226.](http://images.novusbio.com/fullsize/eIF2A-Antibody-Western-Blot-NBP1-84879-img0008.jpg "Western Blot: eIF2A Antibody [NBP1-84879] - Analysis in human cell line RPMI-8226.")
![Immunocytochemistry/Immunofluorescence: eIF2A Antibody [NBP1-84879] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/eIF2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84879-img0007.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody [NBP1-84879] - Staining of human rectum shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/eIF2A-Antibody-Immunohistochemistry-Paraffin-NBP1-84879-img0002.jpg)
![Western Blot: eIF2A Antibody [NBP1-84879] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-179](http://images.novusbio.com/fullsize/eIF2A-Antibody-Western-Blot-NBP1-84879-img0006.jpg)
![Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-278202415212468.jpg "Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32408) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29820241411540.jpg)
![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunocytochemistry analysis of Hela cells labeling GRP78/HSPA5 with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29202410484873.png)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0016.jpg "Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human cerebral cortex, placenta, testis and tonsil using Anti-Calnexin antibody NBP1-85520 (A) shows similar protein distribution across tissues to independent antibody NBP1-85519 (B).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0013.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0014.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85520] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85520-img0015.jpg)
![Western Blot: ATF4 Antibody [NBP2-15499] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ATF4-Antibody-Western-Blot-NBP2-15499-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: ATF4 Antibody [NBP2-15499] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF4 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ATF4 Antibody [NBP2-15499] - HeLa cells mock (left) and treated with 2 ug/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: ATF4 protein stained by ATF4 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/ATF4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15499-img0003.jpg)
![Western Blot: SKIV2L Antibody [NBP2-81903] - Analysis of SKIV2L in mouse stomach tissue lysate with SKIV2L antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/SKIV2L-Antibody-Western-Blot-NBP2-81903-img0001.jpg "Western Blot: SKIV2L Antibody [NBP2-81903] - Analysis of SKIV2L in mouse stomach tissue lysate with SKIV2L antibody at 1 ug/ml.")
![Immunohistochemistry: SKIV2L Antibody - BSA Free [NBP2-81903] - Immunohistochemistry of SKIV2L in human stomach tissue with SKIV2L antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81903_rabbit-polyclonal-skiv2l-antibody-2711202419531670.jpg)
![Immunocytochemistry/ Immunofluorescence: SKIV2L Antibody - BSA Free [NBP2-81903] - Immunofluorescence of SKIV2L in human stomach tissue with SKIV2L antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-81903_rabbit-polyclonal-skiv2l-antibody-2711202420333860.jpg)
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202414471571.jpg "Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Image of eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-262202415475635.jpg)
![Western Blot: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Positive Western Blot detected in Hela whole cell lysate (treated with Calyculin A or EGF). All lanes: eIF2 alpha/EIF2S1 [p Ser51] Antibody at 1.48ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 36 KDa Observed band size: 36 KDa](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202411442641.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) [NBP3-26427] - Staining of A549 cells (treated with 100ng/ml EGF for 20min) with eIF2 alpha/EIF2S1 [p Ser51] Antibody (1C6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26427_rabbit-eif2-alpha-eif2s1-p-ser-51-mab-1c6-282202412493624.jpg)
![SDS-Page: Recombinant Human GRP78/HSPA5 Protein [H00003309-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-GRP78-HSPA5-Protein-SDS-Page-H00003309-P01-img0002.jpg "SDS-Page: Recombinant Human GRP78/HSPA5 Protein [H00003309-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - ERN1 monoclonal antibody (M02), clone 8D12. Analysis of ERN1 expression in LNCaP. Theoretical molecular weight: 110 kDa.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Western-Blot-H00002081-M02-img0008.jpg "Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - ERN1 monoclonal antibody (M02), clone 8D12. Analysis of ERN1 expression in LNCaP. Theoretical molecular weight: 110 kDa.")
![Immunocytochemistry/Immunofluorescence: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of monoclonal antibody to ERN1 on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Immunocytochemistry-Immunofluorescence-H00002081-M02-img0006.jpg)
![Western Blot: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of ERN1 expression in HeLa. Theoretical molecular weight: 110 kDa. Observed molecular weight may vary.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Western-Blot-H00002081-M02-img0001.jpg)
![Proximity Ligation Assay: IRE1 alpha Antibody (8D12) [H00002081-M02] - Analysis of protein-protein interactions between TRAF2 and ERN1. HeLa cells were stained with anti-TRAF2 rabbit purified polyclonal 1:1200 and anti-ERN1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-Proximity-Ligation-Assay-H00002081-M02-img0004.jpg)
![ELISA: IRE1 alpha Antibody (8D12) [H00002081-M02] - IRE1 Antibody (8D12) [H00002081-M02] - Detection limit for recombinant GST tagged ERN1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-8D12-ELISA-H00002081-M02-img0007.jpg)
![Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Western-Blot-NBP2-42176-img0004.jpg "Western Blot: ATF4 Antibody (S360A-24) [NBP2-42176] - Western Blot analysis of Rat Brain showing detection of ~39 kDa (isoform 2) ATF4protein using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~39 kDa (isoform 2).")
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-ATF4Monoclonal Antibody, Clone S360A-24 (NBP2-42176). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-ATF4Monoclonal Antibody (NBP2-42176) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) ATF4Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (S360A-24) [NBP2-42176] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-ATF4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ATF4-Antibody-S360A-24-Immunocytochemistry-Immunofluorescence-NBP2-42176-img0001.jpg)