| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-448SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-06277SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85519-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71309 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77251-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77252-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95252-0.02ml | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| CoraFluor 1 | NBP2-26296CL1 | |||
| Janelia Fluor 525 | NBP2-26296JF525 | |||
| Janelia Fluor 669 | NBP2-26296JF669 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-26296SS | |||
| Azide and BSA Free | NBP2-80716 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89969-25ul | |||
![Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Western-Blot-NB100-448-img0005.jpg "Western Blot: Derlin 1 Antibody [NB100-448] - Detection of Derlin protein in canine KRM microsomes.")
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NB100-448] - Derlin-1 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-448-img0001.jpg)
![Immunohistochemistry: Derlin 1 Antibody [NB100-448] - Staining of human liver.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-NB100-448-img0006.jpg)
![Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of ATF4 expression in transfected 293T cell line by ATF4 monoclonal antibody (M01), clone 2B3.Lane 1: ATF4 transfected lysate(38.6 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ATF4-Antibody-2B3-Western-Blot-H00000468-M01-img0009.jpg "Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of ATF4 expression in transfected 293T cell line by ATF4 monoclonal antibody (M01), clone 2B3.Lane 1: ATF4 transfected lysate(38.6 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (2B3) [H00000468-M01] - Analysis of monoclonal antibody to ATF4 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ATF4-Antibody-2B3-Immunocytochemistry-Immunofluorescence-H00000468-M01-img0007.jpg)
![Western Blot: ATF4 Antibody (2B3) [H00000468-M01] - Western Blot analysis of ATF4 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/ATF4-Antibody-2B3-Western-Blot-H00000468-M01-img0001.jpg)
![ELISA: ATF4 Antibody (2B3) [H00000468-M01] - Detection limit for recombinant GST tagged ATF4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ATF4-Antibody-2B3-ELISA-H00000468-M01-img0010.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-06277-img0005.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-06277] - Detection of Bip/Grp78 in rat liver.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-06277] - BIP/Grp78 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06277-img0006.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band for GRP78 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0008.jpg)
![Simple Western: GRP78/HSPA5 Antibody [NBP1-06277] - Lane view shows a specific band detected for GRP78 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or Sally Sue separation system 12-230kDa (or 66-440kDa).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Simple-Western-NBP1-06277-img0007.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-16749-img0020.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16749-img0018.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat duodenum. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0016.jpg)
![Western Blot: GRP78 Antibody [NBP2-16749] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/GRP78-Antibody-Western-Blot-NBP2-16749-img0002.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 7.5% SDS-PAGE Grp78 antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-16749-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-16749-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP2-16749] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Grp78 protein stained by Grp78 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16749-img0017.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0005.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse brain. Grp78 antibody diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0006.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse testis. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0013.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Rat liver. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0014.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-16749] - Mouse intestine. Grp78 stained by Grp78 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-16749-img0015.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - GRP78/HSPA5 proteins were measured by immunoblotting in epithelioid (EPI BAP1+Â UPN7), biphasic (BIP BAP1-Â UPN16) and sarcomatous (BAP1+Â UPN22) MPM cells. Tubulin was used as a loading control. The figure is representative of 1 out of 3 experiments with similar results. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35197103/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-25520231539516.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: GRP78/HSPA5 Antibody [NBP2-16749] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16749_rabbit-polyclonal-grp78-hspa5-antibody-1610202419405722.jpg)
![Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-85519-img0018.jpg "Western Blot: Calnexin Antibody [NBP1-85519] - LAMP2 released to the cytosol is part of small membrane vesicles. c Sucrose gradient fractions (0.3-2 M) immunoblotted against LAMP2 from cells exposed to LLOMe (1 mM, 2 h). Calnexin is used as an organelle marker. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41419-020-2527-8), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody [NBP1-85519] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85519-img0006.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0017.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human cerebral cortex, placenta, testis and tonsil using Anti-Calnexin antibody NBP1-85519 (A) shows similar protein distribution across tissues to independent antibody NBP1-85520 (B).](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0014.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0015.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-85519] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-85519-img0016.jpg)
![Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Western-Blot-NBP2-02396-img0001.jpg "Western Blot: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Endothelial Lipase.")
![Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endothelial Lipase.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunocytochemistry-Immunofluorescence-NBP2-02396-img0008.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0007.jpg)
![Flow Cytometry: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - HEK293T cells transfected with either Endothelial Lipase Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LIPG antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Flow-Cytometry-NBP2-02396-img0010.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0002.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0003.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0004.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human breast tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0005.jpg)
![Immunohistochemistry-Paraffin: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Staining of paraffin-embedded Human Kidney tissue using anti-Endothelial Lipase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunohistochemistry-Paraffin-NBP2-02396-img0006.jpg)
![Immunoprecipitation: Endothelial Lipase Antibody (OTI1D9) [NBP2-02396] - Lane 1: Negative Control Lane 2: NBP2-02378 Lane 3: NBP2-02380 Lane 4:NBP2-02381 Lane 5: NBP2-02382 Lane 6: NBP2-02394 Lane 7: NBP2-02395 Lane 8: NBP2-02396 Lane 9: NBP2-02397](http://images.novusbio.com/fullsize/Endothelial-Lipase-Antibody-OTI1D9-Immunoprecipitation-NBP2-02396-img0009.jpg)
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Western-Blot-NB100-81896-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using eIF2 alpha (Phospho-Ser51) antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Paraffin-embedded human lung carcinoma tissue.](http://images.novusbio.com/fullsize/eIF2A-[p-Ser51]-Antibody-Immunohistochemistry-Paraffin-NB100-81896-img0011.jpg)
![Western Blot: eIF2A [p Ser51] Antibody [NB100-81896] - Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1: 1000,](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-23420241523258.jpg)
![Immunohistochemistry-Paraffin: eIF2A [p Ser51] Antibody [NB100-81896] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using NB100-81896(left) or the same antibody preincubated with blocking peptide (right).](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-103202523464519.jpg)
![Immunocytochemistry/Immunofluorescence: eIF2A [p Ser51] Antibody [NB100-81896] - Staining of methanol-fixed HeLa cells using NB100-81896](http://images.novusbio.com/fullsize/nb100-81896_rabbit-polyclonal-eif2a-p-ser51-antibody-1132025155593.jpg)
![Western Blot: XBP1 Antibody [NBP1-77252] - PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0003.jpg "Western Blot: XBP1 Antibody [NBP1-77252] - PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.")
![Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77252] - HepG2 cells with XBP-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77252-img0002.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Western-Blot-NBP2-95252-img0008.jpg "Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of U2OS cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251639688.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat pancreas using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0007.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of 293T cells, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/IRE1 alpha Antibody-Western Blot-NBP2-95252-img0009.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Human breast cancer using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0004.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Mouse kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0005.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of PC-12 cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-52202516334127.jpg)
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of NIH/3T3 cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251640267.jpg)
![Immunohistochemistry: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251633413.jpg)
![Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7B11-Western-Blot-NBP2-26296-img0003.jpg "Western Blot: eIF2A Antibody (3A7B11) [NBP2-26296] - Western blot analysis of HeLa (1), NIH3T3 (2), A431 (3), MCF-7 (4), Ntera2 (5), PC12 (6), HepG2 (7), and Cos7 (8) cell lysate using eIF2A antibody at a concentration of 2 ug/ml.")
![Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7B11) [NBP2-26296] - eIF2A antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7B11-Immunocytochemistry-Immunofluorescence-NBP2-26296-img0001.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody (3A7B11) [NBP2-26296] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human breast cancer using eIF2A (3A7B11) antibody at 1:200 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic and perinuclear staining was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7B11-Immunohistochemistry-Paraffin-NBP2-26296-img0005.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining in human placenta and skeletal muscle tissues.. Corresponding HSPA5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining in human placenta and skeletal muscle tissues.. Corresponding HSPA5 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: GRP78/HSPA5 Antibody [NBP1-89969] - Analysis using Anti-HSPA5 antibody NBP1-89969 (A) shows similar pattern to independent antibody NBP1-89968 (B).](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-89969-img0014.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0013.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-89969] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-89969-img0007.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0009.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0011.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP1-89969] - Staining of human rectum shows strong cytoplasmic positivity in glandular and lymphoid cells.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP1-89969-img0012.jpg)