| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-600SS | |||
| Azide and BSA Free | NBP2-80602 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87797-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83256-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73627 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71267 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73645 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-206SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54702-25ul | |||
![Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Nanog-Antibody-Western-Blot-NB110-40660-img0002.jpg "Western Blot: Nanog Antibody [NB110-40660] - Staining of Human Ovary lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Nanog Antibody [NB110-40660] - Staining (green) parts of a colony of induced pluriform stem cells derived from Human Keratinocytes. Antibody at 5 ug/mL. Data kindly provided by CMRB, Center of Regenerative Medicine in Barcelona, Spain.](http://images.novusbio.com/fullsize/Nanog-Antibody-Immunocytochemistry-Immunofluorescence-NB110-40660-img0001.jpg)
![Western Blot: ZMPSTE24 Antibody [NB100-2388] - Detection of ZMPSTE24 in human testis.](http://images.novusbio.com/fullsize/ZMPSTE24-Antibody-Western-Blot-NB100-2388-img0004.jpg "Western Blot: ZMPSTE24 Antibody [NB100-2388] - Detection of ZMPSTE24 in human testis.")
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0004.jpg "Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 using NB100-600.")
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - An intracellular stain was performed on MCF7 cells with BRCA1 Antibody [MU] NB100-600C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0006.jpg)
![Western Blot: BRCA1 Antibody (MU) [NB100-600] - Detection of BRCA1 in MCF-7 whole cell lysate using NB100-600. 2 minute ECL exposure.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Western-Blot-NB100-600-img0003.jpg)
![Flow Cytometry: BRCA1 Antibody (MU) [NB100-600] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using BRCA1 antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/BRCA1-Antibody-MU-Flow-Cytometry-NB100-600-img0005.jpg)
![Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Proteasome-19S-S7-Antibody-Western-Blot-NBP1-87797-img0008.jpg "Western Blot: Proteasome 19S S7 Antibody [NBP1-87797] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
![Immunohistochemistry-Paraffin: Proteasome 19S S7 Antibody [NBP1-87797] - Staining of human hippocampus shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/Proteasome-19S-S7-Antibody-Immunohistochemistry-Paraffin-NBP1-87797-img0009.jpg)
![Simple Western: Proteasome 19S S7 Antibody [NBP1-87797] - Mouse whole spleen lysates at the indicated concentrations were probed with a 1:25 dilution of this Proteasome 19S S7 antibody. Burn-out of the major peak is beginning at 0.5 mg/mL. Simple Western image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Proteasome-19S-S7-Antibody-Simple-Western-NBP1-87797-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: HLTF Antibody [NBP1-83256] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in greeen.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83256-img0007.jpg "Immunocytochemistry/Immunofluorescence: HLTF Antibody [NBP1-83256] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in greeen.")
![Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83256-img0011.jpg)
![Western Blot: HLTF Antibody [NBP1-83256] - Analysis in human cell line HEL.](http://images.novusbio.com/fullsize/HLTF-Antibody-Western-Blot-NBP1-83256-img0006.jpg)
![Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83256-img0008.jpg)
![Immunohistochemistry-Paraffin: HLTF Antibody [NBP1-83256] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HLTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83256-img0009.jpg)
![Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Western-Blot-NBP2-00990-img0019.jpg "Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.")
![Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunocytochemistry-Immunofluorescence-NBP2-00990-img0011.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0018.jpg)
![Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 19S 10B monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Western-Blot-NBP2-00990-img0010.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0012.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0013.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0014.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0015.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human breast tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0016.jpg)
![Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human Kidney tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-1G6-Immunohistochemistry-Paraffin-NBP2-00990-img0017.jpg)
![Immunocytochemistry/Immunofluorescence: Proteasome 19S S5A Antibody [NBP2-19952] - Immunofluorescence analysis of paraformaldehyde-fixed A549, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19952-img0002.jpg)
![Western Blot: Proteasome 19S S5A Antibody [NBP2-19952] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Proteasome-19S-S5A-Antibody-Western-Blot-NBP2-19952-img0001.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241537198.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241538418.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - E6AP levels depend on hRpn10.hRpn10 (a) or E6AP (b) was knocked down in HCT116 cells by four different siRNAs & the cell lysates immunoprobed as indicated. Mock & scrambled control samples are included. beta -actin is used as a loading control in a & d. c Lysates from HCT116 or clone 14 cells expressing myc-hRpn10 constructs were immunoprobed as indicated. d Lysates from HCT116 or clone 13 cells expressing HA-E6AP and/or myc-hRpn10 constructs were immunoprobed as indicated. a–d Antibodies used for immunoprobing are indicated to the left of each panel. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202415291972.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113548.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416113543.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-31020241611358.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - hRpn10 RAZUL contributes E6AP to the proteasome.a Immunoblots of Rpt3 immunoprecipitates or WCE from HCT116 or clone 13 lysates expressing myc-hRpn10 constructs. An asterisk “*” indicates heavy chain antibody. Cyclophilin B (Cyp B) is used as a loading control for WCE samples in a–c & hRpn2 as a positive control for the immunoprecipitation. IgG controls are included. b, d Immunoblots of Rpt3 or IgG (control) immunoprecipitates or WCE of lysates from HCT116 cells transfected with empty vector (as a control) or myc-hRpn10 RAZUL. c Immunoblots of Rpt3 immunoprecipitates or WCE from lysates of HCT116 cells transfected with a scrambled control or siRNA against E6AP. a–d Antibodies used for immunoprobing are indicated to the left of each panel. e Pull-down assay for a commercially available mixture of His6-tagged, non-cleavable K48-linked Ub2/Ub4 with incubation of human 26S proteasome (lane 6), 26S proteasome with equimolar E6AP (lane 7), or just E6AP (lane 8). E6AP or 26S proteasome was added to Ni-NTA agarose resin as negative controls (lanes 4 & 5). K48-linked Ub2/Ub4, E6AP, & 26S proteasome were loaded directly in lanes 1–3, as indicated. f Selected regions from 1D 13C-edited, 1H NMR experiments acquired at 850 MHz & 25 °C for free 13C-AZUL (black) or mixtures with equimolar unlabeled RAZUL (blue) or 26S proteasome (red). The concentration of each sample was 0.3 μM & 200,000 scans were recorded for each experiment. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416121996.jpg)
![Western Blot: S5a/Angiocidin Antibody [NBP2-19952] - A C-terminal domain in hRpn10 binds E6AP AZUL.a Positions of known functional domains within hRpn10 (top) & E6AP isoform II (bottom). Question mark “?” indicates hRpn10 uncharacterized region & the E6AP catalytic cysteine C843 is indicated. b Pull-down assay of His-tagged hRpn10full-length (full), hRpn10196–377 or hRpn10196–306 without (−) or with (+) incubation of E6AP. c1H, 15N HSQC spectra of 0.2 mM 15N-hRpn10305–377 (black) & with twofold molar excess unlabeled AZUL (green). Shifted signals are labeled. d Table summarizing Kd, kon, & koff average values with standard deviations for the hRpn10305–377: AZUL interaction measured by ITC and/or SPR. N/A, not applicable. e HCT116 lysates expressing empty vector, myc-hRpn10 full length, or myc-Rpn10 with RAZUL deleted ( delta 307–377) were subjected to myc-immunoprecipitation with anti-myc-tag nanobody-coupled agarose. Whole cell extracts (WCE) & myc-immunoprecipitates were immunoprobed with the indicated antibodies. Cyclophilin B (Cyp B) is used as a loading control in e & f. f Lysates from HCT116 cells expressing HA-E6AP & the myc-hRpn10 constructs of e were subjected to HA IP followed by immunoblotting with the indicated antibodies. An asterisk “*” indicates non-specific interaction; double asterisk “**” indicates heavy chain antibody. e–f All antibodies used for immunoprobing are indicated to the left of the images. Note that the hRpn10 & E6AP antibodies recognize both endogenous & exogenously expressed protein, causing these panels to show both tagged & endogenous protein. g Schematic representation highlighting interaction domains of hRpn10 including newly identified RAZUL. Source data are provided as a Source Data file. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32157086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-19952_rabbit-polyclonal-s5a-angiocidin-antibody-310202416114714.jpg)
![Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Western-Blot-NBP2-02199-img0015.jpg "Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] Analysis of extracts (35ug) from 9 different cell lines by using anti-PSMD10 monoclonal antibody.")
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Ovary tissue using anti-PSMD10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0013.jpg)
![Flow Cytometry: PSMD10 Antibody (3F6) [NBP2-02199] - Analysis of Jurkat cells, using anti-PSMD10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Flow-Cytometry-NBP2-02199-img0002.jpg)
![Western Blot: PSMD10 Antibody (3F6) [NBP2-02199] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMD10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMD10.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Western-Blot-NBP2-02199-img0003.jpg)
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-PSMD10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0005.jpg)
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-PSMD10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0008.jpg)
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded endometrium tissue using anti-PSMD10 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0010.jpg)
![Immunohistochemistry-Paraffin: PSMD10 Antibody (3F6) [NBP2-02199] - Staining of paraffin-embedded Kidney tissue using anti-PSMD10mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Immunohistochemistry-Paraffin-NBP2-02199-img0011.jpg)
![Flow Cytometry: PSMD10 Antibody (3F6) [NBP2-02199] - Analysis of Hela cells, using anti-PSMD10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PSMD10-Antibody-3F6-Flow-Cytometry-NBP2-02199-img0001.jpg)
![Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S beta 7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 20S beta 7.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Western-Blot-NBP2-01832-img0025.jpg "Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 20S beta 7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 20S beta 7.")
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human prostate tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0023.jpg)
![Western Blot: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Proteasome 20S beta 7 monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Western-Blot-NBP2-01832-img0013.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0014.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0015.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0017.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human breast tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0019.jpg)
![Immunohistochemistry-Paraffin: PSMB7 Antibody (OTI1E1) [NBP2-01832] - Staining of paraffin-embedded Human colon tissue using anti-Proteasome 20S beta 7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Proteasome-20S-beta-7-Antibody-1E1-Immunohistochemistry-Paraffin-NBP2-01832-img0020.jpg)
![Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Knockdown-Validated-NB500-206-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Western blot shows lysates of A549 human lung epithelial parental cell line and Caspase-7 knockout (KO) A549 cell line. PVDF membrane was probed with 1:500 of Mouse Anti-Human Caspase-7 Monoclonal Antibody (Catalog # NB500-106) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for Caspase-7 at approximately 40 kDa (as indicated) in the parental A549 cell line, but is not detectable in the knockout A549 cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - HeLa whole cell lysate.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Western-Blot-NB500-206-img0002.jpg)
![Simple Western: Caspase-7 Antibody (Mch3 1-1-11) [NB500-206] - Simple Western lane view shows a specific band for Caspase 7 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Caspase-7-Antibody-Mch3-1-1-11-Simple-Western-NB500-206-img0003.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0013.jpg "Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: USP14 Antibody (6D6) [H00009097-M04] - Analysis of monoclonal antibody to USP14 on HeLa cell. Antibody concentration 20 ug/ml](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Immunocytochemistry-Immunofluorescence-H00009097-M04-img0008.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0010.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0011.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0012.jpg)
![Western Blot: USP14 Antibody (6D6) [H00009097-M04] - Analysis of USP14 expression in transfected 293T cell line by USP14 monoclonal antibody (M04), clone 6D6. Lane 1: USP14 transfected lysatE (56.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-Western-Blot-H00009097-M04-img0014.jpg)
![ELISA: USP14 Antibody (6D6) [H00009097-M04] - Detection limit for recombinant GST tagged USP14 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/USP14-Antibody-6D6-ELISA-H00009097-M04-img0015.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemisty analysis of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0010.jpg "Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemisty analysis of human endometrium shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemistry analysis of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0007.jpg)
![Western Blot: ATG5 Antibody [NBP2-54702] - Western blot analysis in control (vector only transfected HEK293T lysate) and ATG5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP2-54702-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP2-54702] - Immunocytochemistry analysis of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54702-img0003.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemistry analysis of human tonsil shows moderate to strong cytoplasmic positivity in a subset of lymphoid cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0009.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemistry analysis of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0006.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-54702] - Immunohistochemisty analysis of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells and lymphoid cells.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-54702-img0008.jpg)
