| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-34899-1000ug | |||
| Carrier-Free | NBP2-34899-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75895SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84568-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-32424SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1834SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-261SS | |||
![Western Blot: TRAIL/TNFSF10 Antibody (III6F) [NBP1-97640] - Lane 1: Marker [kDa]. Lane 2: 100ng Recombinant Human TRAIL/TNFSF10 Protein (24kDa). Stained with mouse anti-human TRAIL (III6F; 1ug/mL) overnight at 4C, followed by IR dye 680 goat anti-mouse secondary (LI-COR#926-68070; 1:10,000) for 1 hour at room-temperature.](http://images.novusbio.com/fullsize/TRAIL-TNFSF10-Antibody-III6F-Western-Blot-NBP1-97640-img0002.jpg "Western Blot: TRAIL/TNFSF10 Antibody (III6F) [NBP1-97640] - Lane 1: Marker [kDa]. Lane 2: 100ng Recombinant Human TRAIL/TNFSF10 Protein (24kDa). Stained with mouse anti-human TRAIL (III6F; 1ug/mL) overnight at 4C, followed by IR dye 680 goat anti-mouse secondary (LI-COR#926-68070; 1:10,000) for 1 hour at room-temperature.")
![Immunohistochemistry: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32669) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-298202414161882.jpg "Immunohistochemistry: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/500 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32669) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: NPM1 Antibody (PD00-91) [NBP3-32669] - Immunocytochemistry analysis of LOVO cells labeling NPM1 with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NPM1 antibody (NBP3-32669) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-2920241114844.jpg)
![Flow Cytometry: NPM1 Antibody (PD00-91) [NBP3-32669] - Flow cytometric analysis of Daudi cells labeling NPM1.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32669, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32669_rabbit-npm1-mab-pd00-91-2920241637216.jpg)
![Western blot analysis on different lysates with NBP3-32669 at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: A431 cell lysate Lane 4: C2C12 cell lysate Lane 5: RAW264.7 cell lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32669 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32669_-western-blot-639204594551437476.jpg)
![Western blot analysis of PC-12 cell lysates with NBP3-32669 at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 38 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32669 at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32669_-western-blot-639204597794841873.jpg)
![Western Blot: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Western blot analysis of p63/TP73L on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-32703, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-27820241631652.jpg "Western Blot: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Western blot analysis of p63/TP73L on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NBP3-32703, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p63/TP73L antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32703, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-298202413585578.jpg)
![Immunocytochemistry/ Immunofluorescence: p63/TP73L Antibody (SC06-31) [NBP3-32703] - ICC staining of p63/TP73L in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (NBP3-32703, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-2920241192350.jpg)
![Flow Cytometry: p63/TP73L Antibody (SC06-31) [NBP3-32703] - Flow cytometric analysis of p63/TP73L was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (NBP3-32703, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32703_rabbit-p63-tp73l-mab-sc06-31-29202416453240.jpg)
![Western blot analysis on different lysates with NBP3-32703 at 1/1,000 dilution. Lane 1: A431 cell lysate (15 µg/Lane) Lane 2: MCF7 cell lysate (low expression) (15 µg/Lane) Lane 3: HaCaT cell lysate (15 µg/Lane) Lane 4: Mouse skin tissue lysate (30 µg/Lane) Lane 5: Rat skin tissue lysate (30 µg/Lane) Predicted band size: 77 kDa Observed band size: 60-70 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32703 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32703_-western-blot-639204590071660980.jpg)
![SDS-Page: Human Bcl-xL Protein [NBP2-34899]](http://images.novusbio.com/fullsize/Human-Bcl-xL-Protein-SDS-Page-NBP2-34899-img0001.jpg "SDS-Page: Human Bcl-xL Protein [NBP2-34899]")
![Immunohistochemistry-Paraffin: Ret Antibody [NBP1-84568] - Staining of human gastrointestinal shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Ret-Antibody-Immunohistochemistry-Paraffin-NBP1-84568-img0011.jpg "Immunohistochemistry-Paraffin: Ret Antibody [NBP1-84568] - Staining of human gastrointestinal shows strong membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Ret Antibody [NBP1-84568] - Staining of human adrenal gland shows cytoplasmic membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Ret-Antibody-Immunohistochemistry-Paraffin-NBP1-84568-img0008.jpg)
![Immunohistochemistry-Paraffin: Ret Antibody [NBP1-84568] - Staining of human cerebellum shows strong membranous positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Ret-Antibody-Immunohistochemistry-Paraffin-NBP1-84568-img0009.jpg)
![Immunohistochemistry-Paraffin: Ret Antibody [NBP1-84568] - Staining of human endometrium shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Ret-Antibody-Immunohistochemistry-Paraffin-NBP1-84568-img0010.jpg)
![Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226] - Detection of Human Phospho BRCA1 (S1423) by Western Blot. Samples : Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1423) antibody NB100-226 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1423]-Antibody-Western-Blot-NB100-226-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226] - Detection of Human Phospho BRCA1 (S1423) by Western Blot. Samples : Whole cell lysate (50 ug) from HeLa cells treated with 100 uM EPE for 4 hours (+) or mock treated (-) cells. Antibody: Affinity purified rabbit anti-Phospho BRCA1 (S1423) antibody NB100-226 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Western Blot: BRCA1 [p Ser1423] Antibody [NB100-226] - Detection of Brca1 phospho Ser 1423 by Western blot using NB 100-226. Sample whole cell lysate (25 mcg/lane) from HeLa cells treated with 10 Gy IR or untreated. NB 100-226 was used at 1:2500 dilution.](http://images.novusbio.com/fullsize/BRCA1-[p-Ser1423]-Antibody-Western-Blot-NB100-226-img0002.jpg)
![Western Blot: RIPK2 Antibody [NBP1-32424] - Total protein from human HeLa, A431 and Raji cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RIPK2 (NBP1-32424) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/RIPK2-Antibody-Western-Blot-NBP1-32424-img0009.jpg "Western Blot: RIPK2 Antibody [NBP1-32424] - Total protein from human HeLa, A431 and Raji cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-RIPK2 (NBP1-32424) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: RIPK2 Antibody [NBP1-32424] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-RIPK2 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/RIPK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32424-img0008.jpg)
![Immunohistochemistry-Paraffin: RIPK2 Antibody [NBP1-32424] - Analysis of a FFPE tissue section of human placenta using 1:200 dilution of RIPK2 (NBP1-32424) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This RIPK2 antibody generated cytoplasmic reactivity in most trophoblastic cells.](http://images.novusbio.com/fullsize/RIPK2-Antibody-Immunohistochemistry-Paraffin-NBP1-32424-img0007.jpg)
![Immunohistochemistry-Paraffin: Daxx Antibody - Unpurified [NB100-56136] - Daxx Antibody [NB100-56136] - Tissue sections of human prostate cancer (A and B) stained for Daxx expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/Daxx-Antibody-Immunohistochemistry-Paraffin-NB100-56136-img0001.jpg "Immunohistochemistry-Paraffin: Daxx Antibody - Unpurified [NB100-56136] - Daxx Antibody [NB100-56136] - Tissue sections of human prostate cancer (A and B) stained for Daxx expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.")
![Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0013.jpg "<b>Biological Strategies Validation. </b>Western Blot: EMSY Antibody [NB100-545] - Detection of Human EMSY by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Section of human prostate carcinoma. Affinity purified rabbit anti-Emsy used at a dilution of 1:100 (1 ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0015.jpg)
![Western Blot: EMSY Antibody [NB100-545] - Nuclear extract (NE) from HeLa cells.](http://images.novusbio.com/fullsize/EMSY-Antibody-Western-Blot-NB100-545-img0010.jpg)
![Immunohistochemistry-Paraffin: EMSY Antibody [NB100-545] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-EMSY used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunohistochemistry-Paraffin-NB100-545-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: EMSY Antibody [NB100-545] - Detection of human EMSY by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-EMSY antibody NB100-545 (lot NB100-545-2) used for IP at 6 ug/mg lysate. EMSY was also immunoprecipitated by a previous lot of this antibody (lot NB100-545-1) and another rabbit anti-EMSY antibody. For blotting immunoprecipitated EMSY, NB100-545 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/EMSY-Antibody-Immunoprecipitation-NB100-545-img0014.jpg)
![Western Blot: Gankyrin Antibody [NB100-1834] - Analysis of Gankyrin in HeLa cell lysate. ECL detection in 20 seconds.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Western-Blot-NB100-1834-img0003.jpg "Western Blot: Gankyrin Antibody [NB100-1834] - Analysis of Gankyrin in HeLa cell lysate. ECL detection in 20 seconds.")
![Immunocytochemistry/Immunofluorescence: Gankyrin Antibody [NB100-1834] - Gankryrin antibody (1:500) was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1834-img0006.jpg)
![Flow (Intracellular): Gankyrin Antibody [NB100-1834] - Intracellular staining of Jurkat cells (1 x 10^6 cells/ml) with Gankyrin antibody (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/Gankyrin-Antibody-Flow-Intracellular-NB100-1834-img0009.jpg)
![Western Blot: PUMA Antibody [NB500-261] - Detection of the beta isoform of PUMA in HL-60 whole cell lysate using NB 500-261(4 ug/ml). ECL detection at a 30 minute exposure.](http://images.novusbio.com/fullsize/PUMA-Antibody-Western-Blot-NB500-261-img0003.jpg "Western Blot: PUMA Antibody [NB500-261] - Detection of the beta isoform of PUMA in HL-60 whole cell lysate using NB 500-261(4 ug/ml). ECL detection at a 30 minute exposure.")
![Immunocytochemistry/Immunofluorescence: PUMA Antibody [NB500-261] - The PUMA antibody was tested at a 1:20 dilution in HeLa cells against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue).](http://images.novusbio.com/fullsize/PUMA-Antibody-Immunocytochemistry-Immunofluorescence-NB500-261-img0004.jpg)