| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2214SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-00588SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61685-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38243-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88858-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61686-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79911 | |||
![Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jumonji/JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes. Immunoblot of nucleosomal pull downs from nuclear extract. Total nuclear extract (input) and pull down using streptavidin beads, unmodified nucleosomes or nucleosomes modified by H2AK119u1/H2AK119u1(I44A) were probed with the indicated antibodies using extracts from WT cells (left) or Jumonji/JARID2 KO mESCs (right). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13661) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Knockout-Validated-NB100-2214-img0009.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jumonji/JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes. Immunoblot of nucleosomal pull downs from nuclear extract. Total nuclear extract (input) and pull down using streptavidin beads, unmodified nucleosomes or nucleosomes modified by H2AK119u1/H2AK119u1(I44A) were probed with the indicated antibodies using extracts from WT cells (left) or Jumonji/JARID2 KO mESCs (right). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms13661) licensed under a CC-BY license.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 antibody was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2214-img0001.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Jarid2 expression in naive and activated (overnight) murine B cells. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Western-Blot-NB100-2214-img0008.jpg)
![Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NB100-2214] - Tested in mouse brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunohistochemistry-Paraffin-NB100-2214-img0006.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - Detection of JARID2 protein in E16 mouse brain lysate using 2ug/ml of NB100-2214.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Western-Blot-NB100-2214-img0005.jpg)
![Simple Western: Jumonji/JARID2 Antibody [NB100-2214] - Simple Western lane view shows a specific band for JARID2 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Simple-Western-NB100-2214-img0007.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NB100-2214] - JARID2 N-terminus mediates direct interaction with H2AK119u1-modified nucleosomes(a) Immunoblot of nucleosomal pull downs from nuclear extract. Total nuclear extract (input) & pull down using streptavidin beads, unmodified nucleosomes or nucleosomes modified by H2AK119u1/H2AK119u1(I44A) were probed with the indicated antibodies using extracts from WT cells (left) or JARID2 KO mESCs (right). Full blots are shown in Supplementary Fig. 9. (b) JARID2 1–530 binds to H2AK119u1-modified mononucleosomes (yellow) more tightly than unmodified (blue) or H2AK199u1(I44A) (red) as monitored by biolayer interferometry (n=3). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27892467), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-2214_rabbit-polyclonal-jumonji-jarid2-antibody-310202415392539.jpg)
![Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody NB600-250 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-250-img0015.jpg "Western Blot: ASH2L Antibody [NB600-250] - Detection of human ASH2 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ASH2 antibody NB600-250 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-250] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ASH2used at a dilution of 1:1,000 ( 1ug/ml).Detection: DAB](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-NB600-250-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ASH2L Antibody [NB600-250] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ASH2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-250-img0012.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Analysis of HNF1a transcriptional activity in hepatocytes following transforming growth factor B (TGFB) treatment. (B) Western blot analysis for HNF1a in protein extracts from hepatocytes in a time-course experiment following TGFB treatment. a-Glyceraldehyde 3-phosphate dehydrogenase was used as loading control. Magnification 20x. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fphar.2019.00942/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP1-33596-img0023.jpg "<b>Biological Strategies Validation. </b>Western Blot: HNF1 Antibody [NBP1-33596] - Analysis of HNF1a transcriptional activity in hepatocytes following transforming growth factor B (TGFB) treatment. (B) Western blot analysis for HNF1a in protein extracts from hepatocytes in a time-course experiment following TGFB treatment. a-Glyceraldehyde 3-phosphate dehydrogenase was used as loading control. Magnification 20x. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fphar.2019.00942/full) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP1-33596] - TGFB overrides HNF1a constitutive expression. Immunofluorescence analysis of hepatocytes transfected with pLCPX-HNF1aMyc (HNF1) or the empty vector (CTR), treated with TGFB for 24 hr or left untreated (NT). . Cells were stained with anti-E-cadherin (green) or anti-HNF1a antibody (red). Magnification 20x. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fphar.2019.00942/full) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33596-img0022.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP1-33596-img0018.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP1-33596-img0003.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Rat tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:1000.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP1-33596-img0014.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - detects HNF1 alpha protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.HNF1 alpha stained by HNF1 alpha antibody [N1N3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP1-33596-img0020.jpg)
![Immunoprecipitation: HNF1 Antibody [NBP1-33596] - Immunoprecipitation of HNF1 alpha protein from HepG2 whole cell extracts using 5 ug of HNF1 alpha antibody [N1N3]. Western blot analysis was performed using HNF1 alpha antibody [N1N3]. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunoprecipitation-NBP1-33596-img0016.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - (A) Analysis of CBP/p300 DNA binding activity after TGF beta treatment. qPCR analysis of chromatin immunoprecipitated with anti-CBP/p300 antibody from hepatocytes untreated or treated with TGF beta (for 5 h) was performed. HNF1 alpha consensus regions embedded in the indicated target gene promoters were analyzed. A HNF1 alpha nonbound region of Neurogenin 1 promoter was utilized as negative control. Data are normalized to total chromatin input & background (control immunoprecipitation with IgG) & expressed as % input. Mean ± SEM of qPCR data obtained in triplicate from three independent experiments are reported. (B)In vivo coimmunoprecipitation of HNF1 alpha & CBP/p300 in hepatocytes untreated or treated with TGF beta (for 5 h). Cells were lysed, immunoprecipitated with anti-HNF1 alpha antibody, & then analyzed by Western blotting with the indicated antibodies. As control, the immunoprecipitation with normal goat IgG was performed. TCE, total cell extracts. Densitometric analysis of WB data from three independent experiments is shown. (C) Analysis of HNF1 alpha protein PTMs following TGF beta treatment. Nuclear extracts from control hepatocytes (upper panel) & HNF1 alpha Myc-overexpressing hepatocytes treated for 3 h with TGF beta (lower panel) or left untreated (middle panel). Samples were separated by two-dimensional gel electrophoresis followed by Western blotting with anti-Myc-Tag antibody. HNF1 alpha -specific spots are indicated by the arrow. The appearance of multiple spots in TGF beta -treated hepatocytes can be observed. **p Sample: Paraffin-embedded mouse colon.HNF1 stained by HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242021969.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP1-33596] - HNF1 antibody [N1N3] detects HNF1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.HNF1 stained by HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242011479.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-910202420122014.jpg)
![Immunocytochemistry/ Immunofluorescence: HNF1 Antibody [NBP1-33596] - HNF1 antibody [N1N3] detects HNF1 protein at nucleus by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HNF1 stained by HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242041265.jpg)
![Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 antibody [N1N3] (NBP1-33596) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-91020242091424.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: HNF1 Antibody [NBP1-33596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [N1N3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-33596_rabbit-polyclonal-hnf1-antibody-1610202419414518.jpg)
![Western Blot: ASH2L Antibody (10B6U5) [NBP3-16505] - Analysis of extracts of various cell lines, using ASH2L Rabbit mAb (NBP3-16505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/ASH2L-Antibody-10B6U5-Western-Blot-NBP3-16505-img0007.jpg "Western Blot: ASH2L Antibody (10B6U5) [NBP3-16505] - Analysis of extracts of various cell lines, using ASH2L Rabbit mAb (NBP3-16505) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded human cervix cancer tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-72202512435315.jpg)
![Immunocytochemistry/ Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Confocal imaging of C2C12 cells using ASH2L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-72202512432043.jpg)
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded mouse brain tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-7220251245375.jpg)
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded rat brain tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-72202512432083.jpg)
![Immunocytochemistry/ Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Confocal imaging of NIH/3T3 cells using ASH2L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-7220251245335.jpg)
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded human colon carcinoma tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-7220251245353.jpg)
![Immunohistochemistry: ASH2L Antibody (10B6U5) [NBP3-16505] - Immunohistochemistry analysis of ASH2L in paraffin-embedded mouse lung tissue using ASH2L Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-72202512435389.jpg)
![Immunocytochemistry/ Immunofluorescence: ASH2L Antibody (10B6U5) [NBP3-16505] - Confocal imaging of paraffin-embedded Mouse colon tissue using ASH2L Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16505_rabbit-ash2l-mab-10b6u5-7220251245345.jpg)
![Western Blot: Jumonji/JARID2 Antibody [NBP3-00588] - Total protein from human U2OS and NTERA-2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Jumonji (NBP3-00588) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Western-Blot-NBP3-00588-img0003.jpg "Western Blot: Jumonji/JARID2 Antibody [NBP3-00588] - Total protein from human U2OS and NTERA-2 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Jumonji (NBP3-00588) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: Jumonji/JARID2 Antibody [NBP3-00588] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Jumonji/JARID2 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-00588-img0002.jpg)
![Immunohistochemistry-Paraffin: Jumonji/JARID2 Antibody [NBP3-00588] - Analysis of a FFPE tissue section of mouse brain using 1:200 dilution of Jumonji / JARID2 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Jumonji / JARID2 antibody generated nuclear staining.](http://images.novusbio.com/fullsize/Jumonji-JARID2-Antibody-Immunohistochemistry-Paraffin-NBP3-00588-img0001.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0006.jpg "Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry-Paraffin: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of rectum cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Immunohistochemistry-NBP2-61685-img0004.jpg)
![Flow Cytometry: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of K562 cells using ASH2L mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Flow-Cytometry-NBP2-61685-img0002.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0005.jpg)
![Western Blot: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis using ASH2L mouse mAb against K562 (1) and F9 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Western-Blot-NBP2-61685-img0007.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody (6F6B9) [NBP2-61685] - Analysis of colon cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-Immunohistochemistry-NBP2-61685-img0003.jpg)
![ELISA: ASH2L Antibody (6F6B9) [NBP2-61685] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6F6B9-ELISA-NBP2-61685-img0001.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining in human small intestine and lymph node tissues . Corresponding HNF1A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0012.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining in human small intestine and lymph node tissues . Corresponding HNF1A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: HNF1 Antibody [NBP2-38243] - Analysis in human cell lines Caco-2 and U2OS using anti-HNF1A antibody. Corresponding HNF1A RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/HNF1-Antibody-Western-Blot-NBP2-38243-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: HNF1 Antibody [NBP2-38243] - Staining of human cell line CACO-2 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38243-img0005.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0008.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining of human liver shows weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0009.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining of human lymph node shows no positivity in lymphoid cells.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0010.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody [NBP2-38243] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HNF1-Antibody-Immunohistochemistry-Paraffin-NBP2-38243-img0011.jpg)
![Western Blot: ASH2L Antibody [NBP1-88858] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP1-88858-img0010.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ASH2L Antibody [NBP1-88858] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ASH2L Antibody [NBP1-88858] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88858-img0008.jpg)
![Immunohistochemistry-Paraffin: ASH2L Antibody [NBP1-88858] - Staining of human tonsil shows nuclear positivity in non-germinal center.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-Paraffin-NBP1-88858-img0007.jpg)
![Western Blot: ASH2L Antibody [NBP1-88858] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NBP1-88858-img0006.jpg)
![Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Western-Blot-NB600-281-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: ASH2L Antibody [NB600-281] - Detection of Human ASH2on HeLa whole cell lysate using NB600-281. ASH2 was also immunoprecipitated with rabbit anti-ASH2 antibody NB600-250.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ASH2L Antibody [NB600-281] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- ASH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/ASH2L-Antibody-Immunohistochemistry-Paraffin-NB600-281-img0013.jpg)
![Chromatin Immunoprecipitation: ASH2L Antibody [NB600-281] - ChIP-chip scatter plot of anti-Ash2 enriched DNA binding sites versus input reference DNA. A. 10 ug of NB600-281 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). Immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products abeled with fluorescent dNTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-Ash2 ChIP, normal rabbit IgG showed little enrichment.](http://images.novusbio.com/fullsize/ASH2L-Antibody-Chromatin-Immunoprecipitation-NB600-281-img0009.jpg)
![Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Western-Blot-NBP2-61686-img0004.jpg "Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against human ASH2L (AA: 493-628) recombinant protein. (Expected MW is 41.6 kDa)")
![Immunohistochemistry: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis of paraffin-embedded rectum cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Immunohistochemistry-NBP2-61686-img0003.jpg)
![Western Blot: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis using ASH2L mAb against HEK293 (1) and ASH2L (AA: 493-628)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Western-Blot-NBP2-61686-img0005.jpg)
![Immunohistochemistry: ASH2L Antibody (6B10H10) [NBP2-61686] - Analysis of paraffin-embedded esophageal cancer tissues using ASH2L mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-Immunohistochemistry-NBP2-61686-img0002.jpg)
![ELISA: ASH2L Antibody (6B10H10) [NBP2-61686] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ASH2L-Antibody-6B10H10-ELISA-NBP2-61686-img0001.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Small Intestine stained with HNF1 Antibody (HNF1A/2087).](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Immunohistochemistry-Paraffin-NBP2-79777-img0003.jpg "Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Small Intestine stained with HNF1 Antibody (HNF1A/2087).")
![Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Immunohistochemistry-Paraffin-NBP2-79777-img0001.jpg)
![Immunohistochemistry-Paraffin: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Formalin-fixed, paraffin-embedded human Pancreas stained with HNF1 Antibody (HNF1A/2087).](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Immunohistochemistry-Paraffin-NBP2-79777-img0002.jpg)
![Protein Array: HNF1 Antibody (HNF1A/2087) [NBP2-79777] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HNF1 Antibody (HNF1A/2087). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/HNF1-Antibody-HNF1A-2087-Protein-Array-NBP2-79777-img0004.jpg)
![Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-278202416285253.jpg "Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32486) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-298202414304155.jpg)
![Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunocytochemistry analysis of NCCIT cells labeling Jumonji/JARID2 with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-29202410484844.jpg)